We narrowed to 1,006 results for: RING;

...resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency in a human cell...and ABE7.10. They quantified genomic editing by measuring the reduction in protein expression of these six...

...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability ...Nanoblades editing efficiencies of primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods...

...hold great promise for improving or potentially curing human diseases that were previously thought to ...as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials, wild type AAV...

... of Research (FoR) symposia held across the USA during this time have revealed that young scientists are...Biochemistry and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments...

...and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination at the cellular level...Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector...

...spread. They can be inherited by daughter cells during cellular reproduction, similar to the host’s chromosome...metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain the genes for bacteria to form...

...change (facilitated by the flexible linker) that brings the fluorescent units of the FRET pair together... and diabetes mortality determined by nutrition during parents' and grandparents' slow growth period."...

...options are available to you, it may be worth considering an antibody raised in a different species.    ... strategically plan primary/secondary antibody pairing such that each target has a unique species or isotype...

...supernumerary preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards...produced Ethical concern High. Embryos are destroyed during ESC isolation. Low Chances of post-transplantation...

... use in the protocol, which cover 3 naturally-occurring nanobody structures (concave, loop, and convex...translated protein (sybody), ribosome, and mRNA. During the screening process, displayed sybodies that ...

...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of...

...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand...

...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging...

...FPs that dimerize can form dynamic contacts that bring apposing membranes together and can wrap the membranes...reporter that undergoes a conformational change to bring two FPs on a single protein closer together or further...

...to dicot species. In addition, they generated offspring tomato plants that inherited the genomic edit ...If they do (or if they don't!), please consider sharing your experience with other researchers by contributing...

...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

...downstream flanking sequences to their input string (Figure 2). This string can then be used as input for the prediction...

...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ...

...similar resource for those involved in monocot engineering projects. The MoClo tools will work in any dicot...think plant biology is massively underfunded considering how important it is. I would really like to reach...

...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...