Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,043 results for: STI;

Showing: 741 - 760 of 1043 results

  1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting...PEG) mediated transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. ... to perform, often show low efficiency, while biolistic bombardment, which can have high efficiency, requires...

  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...specific genomic region using the CRISPR system, consisting of dCas9 and targeting gRNA, to identify molecular...vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic ...2017). This upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the...

  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family of transposons. Insertion...(Fig. 3), multiplexed insertions into multiple distinct targets could be achieved (Vo et al., 2020). VchINT... editing. Leveraging a unique marriage between distinct biological elements, CRISPR-transposons excel ...

  4. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...its first steps! However, retroviral vectors can still direct the translation of proteins because they ...and transduction by vectors (which we sometimes still call “infection” as a shorthand, although it is ... the viral RT protein (Faras et al., 1973). Interestingly, retroviruses are generally only “pseudodiploid...

  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...ll prevent binding to any target protein that is still on the inside of the cell. However, assuming you...hidden epitopes. However, keep in mind that you will still contend with many of the same drawbacks to each ...early steps don’t even involve antibodies, they are still critical to the outcome of your IF experiment and...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    ...recolonize the niche, reducing the chance of an opportunistic infection like for organisms like C. difficile...Yosef et al.’s proof-of-concept work targeted two distinct beta-lactam resistance genes, again with very ...PubMed Central: PMCID PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering...

  7. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP are recent examples. We also...University School of Medicine), Europe (Basel Institute for Immunology), and Japan (University of Tokyo...early research career. I've found that research institutes in different places have very different cultures...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish...A. Victoria and expressed in any organism while still maintaining fluorescence. The protein structure,...cytometry that separates mixtures of cells into distinct populations based on fluorescent signal. Thus,...

  9. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...tactics to plan your work and make this happen. Be realistic and: Your co-workers will learn to work within...tasks – try to resist the impulse to control by insisting things always be done your way. Be patient with...help you do your job better. #8 Thou shalt enthusiastically foster independence Your partner can make ...

  10. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...faster, and more efficient than previous methods. Still, generating reliable results from CRISPR edit data...comparing the robustness, accuracy, and speed with existing tools. For example, we examined ICE analysis of...contact us at [email protected] if you have any questions, and check out the Synthego blog for more great...

  11. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...available to researchers around the world. While we still offer many resources for producing vectors from ...vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023, we shared 93,491...difficult as it may be to find answers to such questions, Addgene is deeply interested in pursuing them...

  12. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...numerous fields including research and development, diagnostics, drug discovery, and food safety.  Part of the...antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that they do not ...is non-specific binding or false positives.  If testing a recombinant protein, especially one with tags...

  13. Hot Plasmids - August 2020

    Type
    Blog Post
    ...transcription of the mCitrine gene. mCherry is constitutively expressed. Cells are then cultured and FACS...cells(hPSCs), Lindy Barrett’s lab at the Broad Institute developed fluorescently labeled piggyBac vectors...CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones...

  14. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...Shanghai Institute of Biochemistry, Chinese Academy of Sciences. He joined Shanghai Institutes for Biological...genetic and biochemical mechanisms behind their distinct phenotypes and to design a new generation of engineered...

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    ...control over when and where the enzyme is active, drastically reducing background labeling caused by the biotin...that had been trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023...roles in synapse development, transmission, and plasticity and is a popular marker of excitatory synapses...

  16. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...replication, the negative regulation system cannot distinguish between the different plasmids. The concentration... plasmids from the bacterial pathogens Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis...) Curing of four different plasmids in Yersinia pestis using plasmid incompatibility. Letters in Applied...

  17. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...plasmid due to improper plasmid partitioning, it will still inherit some toxin and antitoxin. However, the antitoxin... serves as a counterselectable marker in the destination vector. Successful cloning occurs when the ccdB...to apply this system as an orally administered diagnostic that can indicate the presence of liver metastasis...

  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...attention, though in reality much of our research still fails to reach our primary target audience – colleagues... disciplines and the years. Which leads to the question of how we can support researchers as they try ...much anyone applying the scientific method to a question. Videos from any discipline are welcome. Up to...

  19. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...specific operational or commercial challenges or questions that clients face imminently. Consulting firms...research, drugs, the diseases they can treat, and diagnostic advancements. Consultants can help these teams...weight with your scientific expertise. Do I have to stick with consulting in the long-term? A few years as...

  20. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...Central PMCID: PMC5568673. 8. Howes, Rob, and Christine Schofield. "Genome engineering using adeno-associated...
Showing: 741 - 760 of 1043 results