We narrowed to 1,075 results for: TOR
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TypeCollection...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ...
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Synthetic Biology - Plant
TypeCollection...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ... -
Synthetic Biology - Worm
TypeCollection...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ... -
Synthetic Biology - Yeast
TypeCollection...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ... -
Synthetic Biology - Sensing and Signaling
TypeCollection...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter Tags PI Publication... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ... -
Top 10 Open Science Developments of 2013
TypeBlog Post..., even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...preference to journal access granted via a public repository or authors’ own web sites. 9) The year 2013... -
Synthetic Biology - Metabolism
TypeCollection...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication ... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent cell lines and any mutations that that...advantages will be revealed in the absence of growth factor. The team assigned the mutations to functional ...significantly increased cell viability, and these “activators” may be the direct drivers of tumor formation... -
9 Tips to Achieve Success in Academia
TypeBlog Post..., and young Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages...different ways to succeed – as loner or gregarious; dictator or laissez-faire; theory or practice; business-like... -
KLF Research Plasmids
TypeCollection...Krüppel-like factors (KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse... -
CRISPR Plasmids - RNA Editing
TypeCollection...catalytically dead Cas13b creates a programmable RNA editor that converts adenosine to inosine in RNA. Since...effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-984–1090 ADAR2 truncation retain... -
An Addgene Summer Internship
TypeBlog Post... gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine, Amrita...exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’... -
Build Your CRISPR Vocabulary
TypeBlog Post...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...array: The section of the bacterial chromosome that stores a genetic record of previously encountered foreign...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...optogenetics blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post...stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory of our... the people at Addgene have become my friends, mentors, and some of my favorite people to talk to. This... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...original policy was straightforward, but cumbersome: depositors had to email us with permission to release their... are left in the hold status and reach out to depositors about them, but we all know how easy it is to...your work. Plus, it’s costly for us to process and store materials that aren’t distributed or for our teams... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post...affiliated TniQ protein recruits transposition cofactors. The transposon proteins integrate a payload sequence... Figure 1: Mechanism of CAST systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...biodistribution therefore introduce additional confounding factors when attempting to interpret signals from these...collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal. Using acoustic reporter...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...match sequence information contributed by the depositor, published backbone sequences, and any known sequences...analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps...experiments like restriction digests. We’ll have a tutorial showing all of the features available on our Snapgene...