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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,076 results for: TOR

Showing: 741 - 760 of 1076 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid ...

  4. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ..., invite them to speak for your organization, mentoring group, or department. Even if it doesn’t work ...ask my benchmate to take my plates out of the incubator because I’d had a baby that night, all he said...

  6. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...PCR to generate the mutation and linearizing your vector (preferably by PCR) for DNA assembly. Further, ...simple as mixing fragments together with linearized vector for seamless joining of multiple mutations spread...

  8. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...delegation tips and practices can be found at the Time Doctor Blog "The Art of Delegation: Developing This Essential...Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development Plan Resources...

  10. Clear and Accessible Writing

    Type
    Blog Post
    ...by screen readers to provide visually impaired visitors with information about images that a sighted reader...be, for example, a graduate student using viral vectors for the first time and learning how to purify AAV...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...about the Developmental Studies Hybridoma Bank, a repository for hybridomas  Learn about monoclonal antibodies...

  13. Antibodies 101: ChIP

    Type
    Blog Post
    ...interest is a low abundance transcription factor or cofactor. In such cases, the gentler enzymatic digestion...

  15. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ...Programmable RNA targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/...

  16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...being studied. Check out Addgene’s validated gRNA repository to find pre-tested gRNA sequences for your next....org  View Addgene's collection of empty gRNA vectors View Addgene's CRISPR resources Request ready-to-use...

  18. RNA Extraction Without A Kit

    Type
    Blog Post
    ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...

  20. Summer SciComm: Preprints

    Type
    Blog Post
    ..., social media, coffee chats with potential collaborators, blogging and more. These each have different...support open peer review or curation models where editors’ comments, reviewers’ comments, and author responses...
Showing: 741 - 760 of 1076 results