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We narrowed to 968 results for: abo

Showing: 741 - 760 of 968 results

  1. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...cycling. The Pros and Cons Researchers used the tools above to complete a 52-piece assembly of a 40 kb T7 phage... help from DAD. The 35-piece assembly mentioned above had a remarkably high 71% fidelity, while the 52...

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...s chromatin status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed...custom design their own engineering plasmid tools in about a week. Taking advantage of Golden Gate cloning,...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...a cell culture model. What we still don't know about Cpf1 In their original paper characterizing Cpf1...Addgene Blog Catch Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac...

  4. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...additional helper sequences (2-3). As alluded to above, termination and polyadenylation of Polymerase II...how the Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids ...

  6. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check out ...

  7. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Listen to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...

  10. Pooled Library Amplifications

    Type
    Blog Post
    ...First, you’ll need to collect specific information about your library: the size of your library (How many...with Addgene. If you have any specific questions about designing your library amplification protocol, feel...

  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...controls must be run in triplicate.  The nice thing about qPCR is that since it measures relative quantification...number, albeit within a range of RQ values as noted above.   In both applications, results can be affected...

  12. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A.,...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

  13. Common Injection Routes in Mice

    Type
    Blog Post
    ...Routes of administration. In H. Hedrich (Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, ...M. A. (2011). Administration of substances to laboratory animals: routes of administration and factors...

  16. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield ...Topic Page Learn How to Verify Your Plasmid Learn about Common Lab E. coli Strains Additional Resources...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

  18. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they can be useful...Important considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals...

  19. MXS Chaining

    Type
    Blog Post
    ...Resources on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids...
Showing: 741 - 760 of 968 results