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  1. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...experiments, therefore, you often must make a choice about what is your top priority: location or efficiency...These Cas proteins have everything we know and love about Cas9, but with broader PAM compatibility. This alternative...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...controls must be run in triplicate.  The nice thing about qPCR is that since it measures relative quantification...number, albeit within a range of RQ values as noted above.   In both applications, results can be affected...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A.,...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

  7. Common Injection Routes in Mice

    Type
    Blog Post
    ...Routes of administration. In H. Hedrich (Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, ...M. A. (2011). Administration of substances to laboratory animals: routes of administration and factors...

  8. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check out ...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield ...Topic Page Learn How to Verify Your Plasmid Learn about Common Lab E. coli Strains Additional Resources...

  11. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Listen to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...

  12. Pooled Library Amplifications

    Type
    Blog Post
    ...First, you’ll need to collect specific information about your library: the size of your library (How many...with Addgene. If you have any specific questions about designing your library amplification protocol, feel...

  17. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... work. After all, a stick-figure internet comic about math probably didn't sound like a great idea to ...  Selecting the method Once you’ve answered the above questions, you can create your short list of options...

  18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

  19. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...famously visited Congress to help increase awareness about the potential of gene therapy!). It would take another...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...
Showing: 741 - 760 of 962 results