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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 956 results for: cha

Showing: 741 - 760 of 956 results

  1. Design Tips for Prime Editing

    Type
    Blog Post
    ...edited and the sequence of the edit, so its design changes for every new prime editing application. And optimizing... L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R....

  2. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...nucleotides are represented equally, that’s only a 1/16 chance of having a PAM at a given site. While it is possible...

  4. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to ...

  5. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...semi-circle (so they can see each other) on comfortable chairs (stools if you have to, but this is tough for women...things started. Give a couple of audience members a chance to participate. For example, at a recent event ...

  6. Transferable Skills: Negotiation

    Type
    Blog Post
    ...are they different from mine? What equipment purchases do we prioritize in a lab with both electrophysiologists...Collide: Managing Successfully Across Cultures by Richard D. Lewis Additional resources on the Addgene...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... tRNA synthetases (aaRS) have been created, characterized, and validated by the Gräslund lab. Recent findings...candidates using a synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence...

  8. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...attending on the larger side? At large conferences, chances are that several talks will be going on at once... for myself like meet ten new people each day. Exchange business cards. When you’re done networking for...

  9. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...setting and no manager will ever be perfect, but the chances of getting a good manager do tend to be higher ...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...

  11. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...Knockout plasmids Homologous recombination is a mechanism to accurately repair harmful double stranded breaks... breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of DNA...

  12. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...cells for long periods of time. This reduces the chance of integration at random locations that could cause..., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J....

  14. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...time consuming to produce, so most labs opt to purchase adenoviruses from dedicated adenoviral production...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...

  16. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since the discovery... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...individual cell clones with different expression characteristics from a population of transfected clones. The...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

  19. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...heard of another double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ...of a single microhomology sequence. For more mechanistic details on HDR and NHEJ, please see the linked...

  20. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...The underlying method was developed in 1902 by Richard Zsigmondy and Henry Siedentopf to enhance the microscopic...using cameras with electron multiplying couple-charged device (EMCCD) or complementary metal-oxide semiconductor...
Showing: 741 - 760 of 956 results