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Showing: 741 - 760 of 1052 results
  1. Creating accessible biology activities in schools with BioBits

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    Blog Post
    ...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...
  2. FPbase: A new community-editable fluorescent protein database

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    Blog Post
    ...sequences and lineages, FRET efficiencies, additional references, etc...) and a more robust database structure...address).  One may also simply attach important references that serve to further characterize a protein ...Currently, all submissions also require a DOI referencing primary literature, and every effort is made ...
  3. Trading Spaces - Visiting Scientist Edition

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    Blog Post
    ...remote collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork...ask questions if you don’t understand something. Offer to help out wherever you can and don’t forget to...
  4. Early Career Researcher Toolbox: Free Tools for Science Writing

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    Blog Post
    ...media, etc.). The free version of Grammarly also offers basic writing suggestions, like alternative word...same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode...
  5. Cultivating Community Science at BosLab

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    Blog Post
    ...both individual and community science projects, we offer classes, workshops, book clubs, seminars, and social...the data to characterize the microbiomes of the different samples. The goal is to not only learn something...something about the microbial diversity in different soil conditions/locations but to engage the local community...
  6. How to Lead a Great Meeting

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    Blog Post
    ...sure to thank everyone for their participation and offer encouragement for the next steps (see “action items...employee’s workload, updates on current projects, and offer feedback. The employee should ask for input on projects...-size-fits all. Feel free to play around with different strategies and find out what works for your organization...
  7. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...blog post we will discuss how mammalian plasmids differ from their bacterial counterparts, including how...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working.... This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin...
  8. A Deep Dive into BioBuilder

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    Blog Post
    ...’s classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused ...opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized programs...point into further STEM exploration.  Further offerings for both STEM teachers and STEM-loving students...
  9. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post
    ...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...
  10. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post
    ...components are still there in real life.  Certainly referring to any DNA within the context of a nucleus as ...chromosomal domains, or how stretches of DNA on different chromosomes interact with each other. Chromatin... Note that histone genes frequently have many different variant names, not all of which can be listed ...
  11. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post
    ...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...
  12. A Quick Guide to a Career in Software Product Management

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    Blog Post
    ...explaining more of her role, which eventually led me to transfer to her team and begin my product management (PM... part of my job is getting to talk to so many different people! I communicate with customers, software... get software product management internships, preferably on teams that run within the agile framework ...
  13. How Addgene Creates Content

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    Blog Post
    ... ideas come from social media conversations, conferences, and questions our scientific support staff receives...line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer ...constantly reviewing the resources we currently offer to identify gaps and add to them as techniques evolve...
  14. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...experiment moving. Happy editing! References and Resources References Hendel, A., Bak, R., Clark, J. et...used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the same issues as your experimental RNA. ...
  15. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...that pathways projecting to the inferior colliculus (IC) are differentially transduced when using the retrograde...significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et al. "A designer...
  16. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post
    ...nature, viruses infect cells and organisms (which we refer to as hosts) via viral particles, the functional...deliver cargo, it’s important to understand their differences to choose the best one for your experiment. ...Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration of...
  17. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post
    ... of interest). Mesophilic microbes are highly preferable (and successful) as host systems since their ...to guess. Think about it like winter tires in different conditions: the more flexible rubber of winter...reagent repositories and the myriad facilities offered by them which help foster a more productive and...
  18. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post
    ...synthesis, and multifragment assembly methods are preferred. No such restriction applies to deletions; using...common methods for site directed mutagenesis   References 1. Trehan, Ashutosh, et al. (2016). “REPLACR-... on Addgene.org Browse Our Molecular Biology Reference Pages Choose Your Perfect Plasmid Backbone Find...
  19. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...
  20. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post
    ...dTomato) that promote interactions between the FPs interfere with native interactions, preventing proper localization...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...
Showing: 741 - 760 of 1052 results