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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 1,041 results for: ins;

Showing: 741 - 760 of 1041 results

  2. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ... the DNA prep is larger. And since the library contains many plasmids, care must be taken to ensure that...plasmids in the library are identical except for the insert; there’s no need to sequence other parts of the...

  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...need to present yourself using a resume. Indeed, instead of having all the pages in a Curricula Vitae to...career, she transitioned into a communications role instead after she completed her MSc in Experimental Medicine...

  4. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system...viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled...

  5. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent... quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some...

  6. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ... coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure... to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...

  7. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...for your workflow. Next time you make a mistake, instead of silently vowing never to do that again (I’ve...incubation periods to have another short "quiet period" instead of immediately running to the next thing.  It may...

  8. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...buffer components could affect the reaction. For instance, sodium azide, a common antimicrobial agent added...relatively rare protein in an experiment with many proteins of interest! Research the brightness of your fluorophores...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    .... Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (Hongquan, et...and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic...

  10. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...communicates your interest in a specific program, and institution. Consequently it’s best to draft a cover letter...or mentor. Several days prior to the interview, inspect your technology, background, and lighting for a...

  12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...gene knockout in vivo and in vitro, as well as to insert targeted mutations through homologous recombination...effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular machinery...

  13. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...many journals use to verify whether a manuscript contains all the necessary information to be of maximal...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    ...biomedical researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter...alike. We transformed this process by aligning institutions with standardized agreements and electronic ...

  17. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... a STAR answer once the interview has started. Instead, I preferred to prepare stories to answer interview...possible. Otherwise, read the video conferencing instructions you received from HR carefully. And even if ...

  18. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...example: in a cell there are proteins which interact with other proteins and with DNA, and these complexes...

  19. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    .... The advantages provided by chemogenetics are instead that it is noninvasive and has longer-lasting effects.... PSAMs themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD) of a...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...in a variety of outcomes including excision or insertion, inversion, translocation or cassette exchange...addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is 5’-TBN-3’ (where...
Showing: 741 - 760 of 1041 results