We narrowed to 1,050 results for: ins;

1. CRISPR Challenges: Standardization and Homology Directed Repair

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   ...CRISPR than just designing a repair template that contains your desired edit, and I want to make sure that...long ssDNA template to increase efficiency of insertion and gene replacement. Care to share your favorite...

2. Multifunctional Neural Fibers: Modulation from Gut to Brain

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   ...sensors, and other common neuroscience tools into the brains of model organisms, including the Anikeeva lab’... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

3. Intro to Our Summer SciComm Series!

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   ...field of science you are communicating from. For instance, a plumber, a physicist, an ambassador to Spain...exclude people outside of the original recipients. Instead, it targets a specific audience - giving consistency...

4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive Activity through Recombinase...protein fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences which brings the...

5. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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   ...natural or synthetic, the researchers looked at intrinsic properties (GC content or rare codon percentage...not rare codons and many genes where there are instances of rare codons, sometimes intentionally modulating...

6. Using Addgene's New Viral Service for Your Research

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   ...Instead of spending time and money producing virus from select vectors in the repository yourself, you...serotypes that can be used to deliver fluorescent proteins to cells as determined by the serotype. You can...

7. Don’t Wait To Launch Your Health Science Startup

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   ...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...company advancement. Be relentless The world is against you.  As I mentioned, you start at a disadvantage...

8. Plasmids 101: NGS Quality Control for Pooled Libraries

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   ... the DNA prep is larger. And since the library contains many plasmids, care must be taken to ensure that...plasmids in the library are identical except for the insert; there’s no need to sequence other parts of the...

9. The Power Behind NGS Plasmid Validation: seqWell

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   ...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...completely unnecessary for sequencing multi-kb DNA inserts and larger targets. The NGS readout also reveals...

10. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ...models for immunogenicity prediction of therapeutic proteins. European journal of pharmaceutics and biopharmaceutics...biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, ...

11. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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    ...reading through a paper to find that there is insufficient information about which reagents were used? ... options using the different PID formats The instructions for how to cite any Addgene plasmid in your ...

12. Summer SciComm Series: A PhD in Science Communication

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    ...never finish a course of antibiotics, choosing instead to save a few days’ worth of capsules to self-medicate...much of the information gained from that research remains inaccessible, behind paywalls and in technical ...

13. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    ... Figure 1: Can you draw Spongebob from these instructions without knowing you're suppose to draw Spongebob..., test out the technology before the conference begins.“There were some concerns of having a stable internet...

14. Resume Writing for Non-academic Science Careers

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    ...need to present yourself using a resume. Indeed, instead of having all the pages in a Curricula Vitae to...career, she transitioned into a communications role instead after she completed her MSc in Experimental Medicine...

15. Top Requested Lentivirus and AAV of 2016

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    ...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system...viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled...

16. Sharing is Caring: But How to Distribute Open Hardware?

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    .... Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (Hongquan, et...and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic...

17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...lengths. Recent alternatives to Cas9 While Cas9 remains the most widely used CRISPR nuclease, recent work...101 blog posts Find more information about Cas proteins Download the CRISPR 101 eBook Resources on Addgene.org...

18. Building and Validating Recombinant Antibodies: A Case Study

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    ... coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure... to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...

19. Sweating the Small Stuff: Details in the Lab

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    ...for your workflow. Next time you make a mistake, instead of silently vowing never to do that again (I’ve...incubation periods to have another short "quiet period" instead of immediately running to the next thing.  It may...

20. Antibodies 101: Buffers, Storage, and Conjugates

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    ...buffer components could affect the reaction. For instance, sodium azide, a common antimicrobial agent added...relatively rare protein in an experiment with many proteins of interest! Research the brightness of your fluorophores...