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We narrowed to 971 results for: lars

Showing: 741 - 760 of 971 results

  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...thermostability could also be used with in vitro molecular bio applications that require high temperature...

  2. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  3. An Addgene Summer Internship

    Type
    Blog Post
    ...day at Addgene last summer, through the La Vida Scholars summer program. I was unsure whether or not I ...which was the impression I got from the La Vida Scholars tour. After some time had passed, I reached out...

  4. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...purification, it will be expressed along with all other cellular proteins within the expression system (bacteria...protein) with amylose resin. The MBP tag, although large, can actually increase expression and solubility...

  6. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...Protein Science at the University of Connecticut. Molecular cloning is the isolation, insertion and amplification... recombinant DNA without sequence alteration. Molecular cloning techniques are some of the most widely...

  7. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ... not seem much different than my second son of similar age. When he came to visit us and watch fellow ...jarring of his body could initiate very painful FOP flare ups. Eventually, without a cure, AJ will begin losing...

  8. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to bring...employee. This is especially true if the problems are large enough that the employee should be aware that they...

  10. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about...Plasmid Pages Resources on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols...

  11. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...maintain fewer copies of plasmids if they contain large inserts or genes that create a toxic product. ...die off. Have any questions? Need something clarified? If so please, post in the comments.  References...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ... all be defined as laypeople when it comes to molecular biology.  Some lay people have a high amount of...more likely to closely read an email sent out to a large listserv or one sent to you and a few other people...

  16. RUBY-Red Siliques

    Type
    Blog Post
    ...equipment. Reporters for studying gene expression have similar drawbacks, often requiring the destruction of plants... for GUS staining or are not suited to studying large samples. RUBY Red But recently, something new has...

  17. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...referred to as TX-TL systems) have been gaining popularity as a synthetic biology tool. These systems allow... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  20. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...challenge in the best of cases. But it can be particularly challenging when your only antibody option is...al., 2013). One issue with this strategy is that large complexes may not permeate through your tissue samples...
Showing: 741 - 760 of 971 results