We narrowed to 997 results for: lars

...Making Your Own Competent Cells” has been extremely popular, generating many additional questions. We’re happy...addgene.org if you are interested. Addgene's Most Popular Posts of 2014 How to Design Your gRNA for CRISPR...

...Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing in plants While there...Cas nucleases. In doing so, they also created a modular toolkit for Cas-mediated genome engineering in ...

... creating and verifying them independently — particularly valuable when budgets are constrained. This ...work, saving time and allowing precious research dollars to go further. Providing essential services: We...

...with our Executive Director, Joanne Kamens. As a regular contributor of careers blog articles, Joanne had...with Director of Biology Lianna Swanson was very popular, with the students excited to see a real working...

...      Some of our lentiviral plasmids include popular envelope and packaging plasmids as well as transfer... great fit for any scientist, whether you use molecular biology tools in your research or not! And learn...

... that 20% of tagged proteins did not localize similarly to their untagged counterparts. These findings... for examining protein-protein interactions, particularly in single-molecule or high-throughput applications...

...Cre-recombination. Read the paper in Frontiers in Molecular Neuroscience Find the AAV-based SaCas9 genome... deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used in diverse bacteria...

...researcher. The future of DayCon DayCon and other similar events show how meaningful science outreach can...event and to expand the conference to accommodate a larger number of attendees. This first-year event’s success...

... possible to quickly and efficiently assemble a large number of building blocks with just two or three...system based on the daily needs of an advanced molecular plant science lab.” Introducing GreenGate As ...

...of NgAgo In particular, we need to thank Pooran Dewari who wrote this month’s most popular blog post on...

... of time, cancers become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke ...drug resistance Wood wanted to find out which particular pathways drive resistance to specific drugs in...

... meeting with someone to ask about a job or a particular position at their company, do NOT call this an... is a good time to ask personal questions about salary, opportunities for advancement, challenges and ...

...in open science this year. Here it goes, in no particular order, my top 10 open science developments, 2013...-respected and high-profile universities. We particularly recommend Eric Lander's intro to biology course...

... Pooled Libraries All CRISPR Pooled Libraries Molecular Biology Reference How do I process my Addgene ...libraries up to 200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly... LB Agar + Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least...

...programs are discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of the programs...programs SITN runs and describes some of the more popular topics from the Science by the Pint series. Chapter...

... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that...

...a network of researchers who may be working on similar projects and should make it easier for them to ...missing an important collection or if you think a particular plasmid or type of plasmid is missing from our...

...strategies for using combinations of AAV-based molecular tools for targeted neuronal manipulation. The ...of interest: Cell-type specific expression of molecular tools and sensors Combinations of classical genetic...

...facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system is the single-color...studies of cell cycle progression, epigenetics and cellular reactions to external stimuli. It could also have...

...Cre-lox recombination is an incredibly useful molecular biology tool, but like any biological system, ... yeast recombinases: KD (from Kluyveromyces drosophilarum), R (from Zygosaccharomyces rouxii), B2 (from...