We narrowed to 979 results for: lars
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TypeBlog Post...dimerization of HLA B27, involved in the onset of spondylarthritis, using Bioluminescence Resonance Energy Transfer...the University of California, specialising in Molecular Biology. She has worked in a range of labs - cancer...
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In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...writer When Hans was finishing up his PhD in Molecular Neuroscience at the University of Iowa, he was...over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am currently getting... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...thermostability could also be used with in vitro molecular bio applications that require high temperature... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog Post...essential cofactors for many tools used in modern molecular biology like restriction enzymes and polymerases...posts Resources on Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post..., as well as to optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an...fluorescent protein plasmids at Addgene Read our Molecular Biology Reference ... -
An Addgene Summer Internship
TypeBlog Post...day at Addgene last summer, through the La Vida Scholars summer program. I was unsure whether or not I ...which was the impression I got from the La Vida Scholars tour. After some time had passed, I reached out... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... -
Antibodies 101: Affinity Tags
TypeBlog Post...purification, it will be expressed along with all other cellular proteins within the expression system (bacteria...protein) with amylose resin. The MBP tag, although large, can actually increase expression and solubility... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...Protein Science at the University of Connecticut. Molecular cloning is the isolation, insertion and amplification... recombinant DNA without sequence alteration. Molecular cloning techniques are some of the most widely... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post... not seem much different than my second son of similar age. When he came to visit us and watch fellow ...jarring of his body could initiate very painful FOP flare ups. Eventually, without a cure, AJ will begin losing... -
Management for Scientists: Giving Feedback
TypeBlog Post...style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to bring...employee. This is especially true if the problems are large enough that the employee should be aware that they... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...attacks. He described one such recent attack as "hilarious". Welcome to Montana! Though sadly I will not ...components, and challenging target genomes, such as the malaria-causing Plasmodium falciparum. Throughout these... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about...Plasmid Pages Resources on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post... are still just as specific to their target as regular antibodies. These properties make nanobodies easier...Wanisch Listen to the C-BERST podcast segment Similarly to the Carr lab, Erik Sontheimer and his group... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined...., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth, metabolism, and antibody... -
Plasmids 101: Origin of Replication
TypeBlog Post...maintain fewer copies of plasmids if they contain large inserts or genes that create a toxic product. ...die off. Have any questions? Need something clarified? If so please, post in the comments. References... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...protein phosphatase that responds to rising intracellular calcium levels within the cell. When levels ... wild type (top panels) and cells with high intracellular Ca2+ (bottom panels) equipped with destabilized...