We narrowed to 822 results for: mak

1. Antibodies 101: Choosing the Right Isotype

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   ...antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity ...

2. Fluorescent Proteins 101: Introduction to FRET

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   ...reviews have collated data from various papers to make the decision easier. 1. Müller, Sara M., et al. ...

3. Open Resources and Plasmid Tools For Studying C. elegans

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   ...produced various resources for the worm community that makes it easier to view and explore the worm. These resources...

4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ...Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ...

5. Hot Plasmids and Viral Preps - January 2021

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   ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

6. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   .... At the same time, there is much opportunity to make headway on long-term efforts to better account for...

7. Tips for Titering Your Lentiviral Preps

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   ... it’s time to move ahead with your infection and make your stable cell line. While we’ve all experienced...

8. Illuminating Epigenetics with A FRET Based Biosensor

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   ... of cytosine to 5-methylcytosine in CpG islands makes  genes less transcriptionally active. The role ...

9. Hot Plasmids: Winter 2025

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   ...and more photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques...

10. Common Injection Routes in Mice

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    ...that they don’t always require needles. If needles make you cringe or your experiment will allow you to ...

11. Viral Vectors 101: Producing Your rAAV

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    ...of rAAV production.  Of course, you will want to make sure that your final stocks only contain rAAV. To...

12. Hot Plasmids and Viral Preps - May 2021

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    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

13. Plasmids 101: Protein Expression

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    ...translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most proteins...

14. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency decreases...

15. Plasmids 101: Degron Tags

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    ...degron will behave with a new protein, but you can make an informed choice! References and Resources References...

16. The Scientific Conference Poster Session: Tips for Success

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    ...interested in viewing which has drawn a large crowd, making it difficult to see the actual poster or interact...

17. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...ability to differentiate into multiple cell types makes them useful models for developmental processes and...

18. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ... protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for...

19. Antibodies 101: Flow Compensation

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    ...will ‘look’ similar on your flow plot. This will make it difficult to properly gate your desired cell ...

20. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...target cells (Beatty et al., 2012). These features make AdV an attractive candidate for in vivo delivery...