Addgene

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Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

... Using this technique, researchers have begun to make important discoveries in various biological fields...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...number of administrative and practical decisions to make. How often will you hold it? How many people do ...
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Type

Blog Post

...ve built a new and improved search engine to help make this task a faster, easier, and frustration-free...
CRISPR 101: RNA Editing with Cas13

Type

Blog Post

...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

...added log-normal noise to each of the terms which make up our distribution, resulting in the following ...
Simplify Cloning with in vivo Assembly

Type

Blog Post

...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

...decide which ones to use? Here are some tips for making those decisions: Always always always look at ...
AAVs in Retinal Gene Therapy

Type

Blog Post

...started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector...
Plasmids 101: Secondary Nanobody Toolbox

Type

Blog Post

...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Type

Blog Post

... DNA compaction and the structural proteins that make it possible, see the appendix DNA is a 3D fractal...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...functional before investing time and resources into making a stable cell line. When expressing a viral construct...
CRISPR-mediated Plant Base Editors

Type

Blog Post

... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...
Bioinformatics at Addgene

Type

Blog Post

...bbdone with our bbduk process. At first, to make sure we understood the concept of channels and implemented...
Transferable Skills Guide: Creativity

Type

Blog Post

...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...
Antibodies 101: Immunoprecipitation

Type

Blog Post

... and high specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must...
Viral Vectors 101: Pseudotyping

Type

Blog Post

...retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity....
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...t simply rely on chance (or random diffusion) to make this happen. There are several different versions...
Plasmids 101: Gateway Cloning

Type

Blog Post

...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...
Mesothelioma - Causes, Symptoms, and Treatment

Type

Blog Post

... long latency period, and rarity of mesothelioma make it different from many other types of cancer. Mesothelioma...

Showing: 741 - 760 of 798 results

Results per page:

Isolating Genomic Regions of Interest with the CRISPR System

A Guide to Starting Your Own Journal Club

Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

CRISPR 101: RNA Editing with Cas13

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Simplify Cloning with in vivo Assembly

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

AAVs in Retinal Gene Therapy

Plasmids 101: Secondary Nanobody Toolbox

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Cpf1: A New Tool for CRISPR Genome Editing

Your Lentiviral Plasmid FAQs Answered

CRISPR-mediated Plant Base Editors

Bioinformatics at Addgene

Transferable Skills Guide: Creativity

Antibodies 101: Immunoprecipitation

Viral Vectors 101: Pseudotyping

Plasmids 101: Plasmid Incompatibility

Plasmids 101: Gateway Cloning

Mesothelioma - Causes, Symptoms, and Treatment

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