We narrowed to 1,024 results for: ple

... look at how we create this content, using the example of our Antibody Guide, which was published alongside...can. We even have a Slack channel just for asking people if they can write or review things and another ...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ...

...specific region of your protein of interest. For example, if you are studying a protein that may bind a ...recognize the protein even though it is present in your sample. Additionally, there is a lower likelihood that...higher than that of polyclonal antibodies. For example, it takes longer to develop a hybridoma than it...

...of both AsLOV2 and the nanobody. As a proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion...manipulation of the signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras...levers to pull to control signaling pathways. For example, if the OptoNB could bind and block a receptor ...

...agriculture, are now synonymous with GM to the point that people cannot imagine any use for GM technology other ... is not food, not medicine, and not mandatory – people can decide for themselves if they want this beautiful...developed a genetic tool for plant engineering, please share it with Addgene to both build the plant synthetic...

...intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies of...and can approximate mRNA concentration within a sample. Using this system, the Collins lab was able to...lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it ...

...affect it. While tumors may be the stars, there's plenty of action outside of tumor cells, and recent studies...deeply connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine...spread and drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes...

...based on size. Whether higher molecular weight complexes are detected will depend on the homodimer affinity...will be problem-free. In figure 1 we provide an example of the OSER assay with a number of fluorescent ...tested. To conclude, we recommend that you try a couple of different fluorescent proteins when protein ...

...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...non-physiological RNA-protein interactions due to sample processing. Require prior knowledge that the protein...levels seen in HHCS patients. These are just three examples of how RaPID can be used to study RNA-protein ...

... practice to compare degradation rates between samples treated with or without an autophagy inhibitor....accumulates in the vacuole. See Figure 2 for an example of what fluorescence looks like for cytoplasmic...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...

...below) Because off-target sequences have less complementarity to the gRNA, they have a higher propensity ...for their on-target and off-target activity at multiple genomic locations in HEK cells. Further testing... Hiroshi, et al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014...

... the LoxP sites, which is more difficult than a simple knock out. Looking for some permanent knock out...transcripts. When transiently introduced, they can deplete endogenous protein levels for 1-3 days. There’... tunable control These systems can be easily implemented for exogenously introduced proteins, as many ...

... further demonstrated that PRIME-del can be “multiplexed” using pooled paired pegRNAs.  Read the paper...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions... without small insertions. But, instead, PEDAR couples this deletion process to a prime editing enzyme...

...ever like to write about a recent plasmid deposit please sign up here.        AAV delivery of split base... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...

...erlotinib. Looking at barcode abundances among multiple replicate populations, they found that similar...went on to show that combination treatment with multiple drugs reveals even smaller preexisting populations...dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015): 440-...

...anything. Viruses are complicated and they perform complex biological tasks, both of which are factors in ...Ugai et al., 2002). If the virus will be used in a couple of days, some researchers prefer to store freshly...: 16510845. 4. Krajden, Mel, et al. "Effect of multiple freeze-thaw cycles on hepatitis B virus DNA and...

...loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads). It is necessary to ...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR ...2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit ...

...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which they named... Multiple Authors...

...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked... Multiple Authors...

...viruses that can infect people, cells, and animals. Some of these viruses are completely inert, but others ...protection are key to lab safety and the same principles apply to viral handling. Gloves are always required...

...have all heard. It’s a truth that extends to both people and the tools we use. Scientists utilize an array... to stop functioning properly or be something completely different than what was originally intended. ...Addgene receives deposited plasmids, they undergo complete plasmid sequencing using an in-house Illumina ...