We narrowed to 1,020 results for: ren
-
TypeBlog Post...sections and brought in more focus on the list of conferences I presented at and placed them under headlines...before I went for the interview. So reading for my current sales position interview was just as important....mechanisms of cell division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on ...
-
Creating accessible biology activities in schools with BioBits
TypeBlog Post...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected]. References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...academic community by funding their travel to a conference of interest. In this post, we’re proud to announce...for human milk oligosaccharides Fatima Enam is currently a graduate student in Thomas J. Mansell’s lab ...cost-effective way to measure the production of HMOs. Current HPLC-MS and fluorophore-based technologies are ... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post...incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused” in ...self-police” or whether the media and academic conference response to He created a positive feedback loop... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post... improve tuberculosis care and control. In her current research, Sheen aims to identify new genes related...related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic...microfabrication technologies to generate a self renewable sensor, a bacterium that can sense contaminants... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for the third base of the PAM so that it now ...and Intensely Desensitizing ChRs) differ from currently used ChRs in that they cause a rapid and almost...researchers identified MerMAIDS from their analysis of different publicly available metagenomic data including ... -
How to Lead a Great Meeting
TypeBlog Post...new and useful information, and invigoration - a renewed energy for continuing the project after leaving...a sense of the employee’s workload, updates on current projects, and offer feedback. The employee should...-size-fits all. Feel free to play around with different strategies and find out what works for your organization... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...-friendly. They experimented with the promoter strength of various components, removing inducible promoters...various scientific or engineering applications. References Refactoring and optimization of light-switchable... -
Viral Vectors 101: Viral Applications
TypeBlog Post...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...can lead to variable expression levels due to differences in copy number. And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below. References 1. Zetsche et al. "Multiplex Gene Editing by ... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...introducing mutations (red nucleotide). Created with BioRender.com. The initial version of EvolvR has an editing...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post... to its involved in cell proliferation and differentiation. Since Ras is a membrane localized GTPase,...expression changes lead to cell proliferation and differentiation. The lab confirmed this OptoNB-SOS activated...for more uses both inside and outside cells. References Gil, Agnieszka A., et al. "Optogenetic control... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below. References Boosting CRISPR/Cas9 multiplex editing capability... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...interest. They also “showed that different guide RNAs have different abilities to turn genes on and off..., transformations require electroporation. References & Further Reading: For more information about... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...Association for Creatine Deficiencies (ACD) is a parent-let non-profit founded with the mission to support...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...data to prove it is worthless. The OSER assay is currently the best way to test a fluorescent protein’s propensity...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...Rounding out the remainder, 2% of postdocs who weren’t going to get a salary increase prior to the injunction...that institutions have sent, with the lack of transparency and poor communication of their plans (many ...guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. ... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...sources. Mode of communication Different modes of communication take up different amounts of mental bandwidth... you're asking of your audience and when things aren't working out, adjust the ask. I used to do a lot... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...recent positions outside of the lab. Granted, I currently work on the Scientific Support Team here at Addgene...resolves the issue. If this sounds like about seven different skills to you, you are not wrong. Problem-solving...how you approach new challenges. Know your strengths! Research experience presents ample opportunities...