1,189 results

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

Addgene recently attended the ASM Microbe 2016 conference in Boston. Check out what we learned!

Learn how DNA methylation may affect the activity of certain restriction endonucleases.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

Learn how to incorporate phosphoserine into your protein of interest to study the effects of phosphorylation.

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

Addgene and Seeding Labs recently awarded plasmids to two researchers in Ghana. Read about their work inside.

We've recently curated a Microbiology Plasmid Collection. Learn what you can find in the collection in this post.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

Guest blogger Kate Palozola gives some tips on how to keep a good and organized lab notebook for bioinformatic analyses.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

In this podcast, learn about Kingdom Kwapata, a Malawian researcher currently developing transgenic crops and new means to produce biofuels.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

Scientists are currently developing disease treatments using CRISPR. Learn about recent developments in the fight against HIV-1 with CRISPR.

Cell culture can be difficult work, follow these tips to keep your cell lines free of contamination.

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

Many commonly used fluorescent proteins form dimers producing artifacts in a variety of experimental settings. Learn how to avoid these artifacts.