1,183 results

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

Addgene and Seeding Labs recently awarded plasmids to two researchers in Ghana. Read about their work inside.

We've recently curated a Microbiology Plasmid Collection. Learn what you can find in the collection in this post.

In this post we directly compare the Argonaute genome editing protein, NgAgo, to the CRISPR endonuclease, Cas9.

Guest blogger Kate Palozola gives some tips on how to keep a good and organized lab notebook for bioinformatic analyses.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

In this podcast, learn about Kingdom Kwapata, a Malawian researcher currently developing transgenic crops and new means to produce biofuels.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

Scientists are currently developing disease treatments using CRISPR. Learn about recent developments in the fight against HIV-1 with CRISPR.

Cell culture can be difficult work, follow these tips to keep your cell lines free of contamination.

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

Many commonly used fluorescent proteins form dimers producing artifacts in a variety of experimental settings. Learn how to avoid these artifacts.

R bodies are tiny, tunable molecule machines that can be used to break membranes. Learn all about them and their potential uses here.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

Addgene is teaming up with nonprofit Seeding Labs to provide plasmids to researchers in developing countries.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.