1,192 results

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

Many commonly used fluorescent proteins form dimers producing artifacts in a variety of experimental settings. Learn how to avoid these artifacts.

R bodies are tiny, tunable molecule machines that can be used to break membranes. Learn all about them and their potential uses here.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

Addgene is teaming up with nonprofit Seeding Labs to provide plasmids to researchers in developing countries.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

Perturb the activity of 17 different signaling pathways using the cancer pathways ORFs kit.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

Can't find a plasmid at Addgene? Suggest new plasmids for the repository by tweeting at us of filling out our "Suggest A Plasmid" form here.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

Learn how Addgene works with you to increase your work's visibility and citations through efforts to organize and curate the repository.

We're recruiting contributors to the Addgene Blog to expand the educational reach of our Plasmids 101 series. Sign up to contribute!

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

Learn about the founding the science communication nonprofit (CINSO) and get tips on thinking about your science career.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.