1,183 results

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

Perturb the activity of 17 different signaling pathways using the cancer pathways ORFs kit.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

Can't find a plasmid at Addgene? Suggest new plasmids for the repository by tweeting at us of filling out our "Suggest A Plasmid" form here.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

Learn how Addgene works with you to increase your work's visibility and citations through efforts to organize and curate the repository.

We're recruiting contributors to the Addgene Blog to expand the educational reach of our Plasmids 101 series. Sign up to contribute!

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

Learn about the founding the science communication nonprofit (CINSO) and get tips on thinking about your science career.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

REPLACR-mutagenesis is a new in vivo method for mutating your plasmid constructs. Learn how REPLACR stacks up against other mutagenesis techniques.

Tired of meetings? Read this blog post for tips on how to run an enjoyable and productive meeting.

Learn about the components of a plasmid page on our website and shows how the different components can be useful to you

A quick guide on the main components of CRISPR: the Cas9 endonuclease and the guide RNA.

Three studies recently showed that CRISPR can be used to treat Duchenne muscular dystrophy in mice. Learn about the work and obstacles to application.

Dr. Michael Koeris is cofounder Biotech startup, Sample 6, and a member of the Addgene Board. Listen to his journey from grad student to entrepreneur.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Addgenie Emma Markham describes her winding career path and provides advice on how you can find a great job after undergrad.