1,189 results

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Addgene's Advisory Board helps steer and promote Addgene in its goal of sharing science. We welcome CRISPR expert, John Doench, to the Advisory Board.

This blog post explores how CRISPR/Cas9 can be used to visualize multiple intrachromosomal loci in vivo and map the 4D nucleome.

This blog post explores ways to gain leadership experience at professional organizations like the National Postdoctoral Association.

This blog post explores how commonly used GFP variants can undergo Green-Red photoconversion.

Interview with Professors Eric Mendenhall and Richard Myers about their CRISPR- based system for tagging any endogenous gene of interest.

Makerspaces provide a place for self-directed research. Learn how members of a Makerspace in Victoria Canada model worm locomotion.

Angela Depace's lab recently described how Individual development plans (IDP's) help advance their research. Read on to learn more about IDPs.

Developments in light sheet fluorescence microscopy (LSFM) and tissue clearing enable researchers to visualize tissues of transgenic and non-transgenic organisms in 3D.

It pays for scientists to have a great resumes and CVs. Follow these simple steps to help get you and your resume noticed by future employers.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

Learn how Tyler J. Ford, an Outreach Scientist at Addgene, transitioned from graduate school to working at Addgene.

Overview of the Sleeping Beauty transposase system and how they are efficient tools for genome engineering.pl

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

The pCri plasmids available through Addgene allow researchers to multiplex the protein purification process saving time and frustration.

Science in the News (SITN), a Harvard group dedicated to making science understandable, recently hosted DayCon, a science conference for the general public.

Addgene plasmid pages now show whether a plasmid has been cited in other articles. Use the "References" link to view the articles citing your plasmid.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.