1,183 results

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

As part of Addgene's Management for Scientists, this post describes the differences between managing vs. leading and the skills associated with each.

Learn about how Addgene has shipped over 500,000 plasmids and where those plasmids go.

Learn more about the Developmental Studies Hybridoma Bank (DSHB), a repository for monoclonal antibodies, that distributes antibodies and their associated hybridoma cell lines.

Read about artists who are turning science into art by using scientific techniques to view the world from another vantage point.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Learn how effective delegation allows you to make the best use of you and your team's time and skills in this Management for Scientists post.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Learn more about E. coli strains used for protein overexpression.

Focusing on the importance of giving feedback to your employees, this is the third installment of a 5 part series on Managing People for Scientists.

Addgene data on CRISPR and synthetic biology plasmids deposited to the repository and distributed around the world.

What is a career coach and how can scientists find the right one for them?

Dora Farkas, career coach for scientists, discusses how to identify difficult supervisors and outlines 7 strategies to resolve conflicts.

Overview of the 2015 Keystone Symposium: Precision Genome Engineering and Synthetic Biology from Addgene's own Eric Perkins, including the state of CRISPR.

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Addgene's Executive Director, Joanne Kamens, shares advice for management of scientists on how to seek feedback from your employees.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Tips for preparing and writing a resume when transitioning from an acamedic to a non-academic science career.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.