1,192 results

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

This blog posts discusses Cas9 gene drives and the appropriate safeguard and confinement strategies scientists should use for these gene drives.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

Tips for writing your scientific manuscript, including how to search for and organize papers.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

Addgene's interviews Chris Voigt of MIT about the intersection of CRISPR and synthetic biology and his new work building circuits with CRISPRs.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

As part of Addgene's Management for Scientists, this post describes the differences between managing vs. leading and the skills associated with each.

Learn about how Addgene has shipped over 500,000 plasmids and where those plasmids go.

Learn more about the Developmental Studies Hybridoma Bank (DSHB), a repository for monoclonal antibodies, that distributes antibodies and their associated hybridoma cell lines.

Read about artists who are turning science into art by using scientific techniques to view the world from another vantage point.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Learn how effective delegation allows you to make the best use of you and your team's time and skills in this Management for Scientists post.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Learn more about E. coli strains used for protein overexpression.

Focusing on the importance of giving feedback to your employees, this is the third installment of a 5 part series on Managing People for Scientists.

Addgene data on CRISPR and synthetic biology plasmids deposited to the repository and distributed around the world.