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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 381 - 390 of 1010 results

  1. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...requires an immense amount of self-knowledge and an unwavering devotion to seeing your ideas become something...CEO of miLab, a Boston-based startup focused on empowering early-stage scientists. You can think of miLab...generation companies.  We launched in the early spring of 2015 and since that time have received about..., including one led by a talented biomedical engineering team from MIT.   I would like to share my own... are everything and you want to leave people remembering your potential as an entrepreneur, not your credentials...

  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring kinase ... visualizing structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All...acceptor molecules are targeted nearby (such as a neighboring house on the same street as the donor), then ...amber i.e. UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid...Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling calibrated FRET distance...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...advancements include work exploring far-red-emitting dyes in bacterial imaging, offering solutions to overcome...Photostability, the ability to retain fluorescent intensity during exposure to light, is another factor to consider...wavelengths, as this is limited by the filter cubes and filtering settings of the equipment. Systematic evaluation...facilitate clear differentiation between multiple dyes during analysis. Image resolution is impacted by several...Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers, R., Bousfield...

  4. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...roles in fluorescent protein development by openly sharing their resources with the research community. To...protein abundance, possibly due to misfolding. But measuring variants one at a time is time consuming and unscalable...use the sensor to answer the question of whether firing of serotonergic neurons (measured using a red calcium...sensor in sleep-related studies and in assays for measuring human serotonin transporter activity. Unger also...dynamics with the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously...

  5. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles...released from the producer cells into the supernatant during the production phase. However, this has not been...at the AAV vectors that are released from cells during production (Maguire et al., 2012). They saw that...: PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells using ...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  6. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...recombination-mediated genetic engineering (find more information on bacterial engineering using CRISPR on our blog..., their interactions and information processing during this condition is crucial. Plasmids expressing ...interactions. Combining synthetic biology with engineering: glowing bacteria and a fish robot to detect ...interesting combinations: biomedicine and astrobiology. During the last 11 years he has studied various aspects...

  7. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ... HeLa cells?), genome engineering and stem cells, and the difficulty of sharing banked iPSCs across international...years, I've been looking forward to seeing how the sharing of his OSKM factors have affected the stem cell...paper as "life changing". Dr. Yamanaka himself, during his keynote address, appeared almost incredulous...into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques are neither...

  8. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ... when scientists bring biases to research and don't collaborate with others to bring diverse perspectives... rules all, the books center around a cast of endearing characters who decide to take matters into their...dueling magicians in the 1800s in England who help bring back actual magic (which had been lost for an age...a book club would force me to read some books exploring new genres, new authors, and new themes. A book...

  9. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...for various scientific or engineering applications.   References Refactoring and optimization of light-switchable...photobiology: why use light? In synthetic biology and bioengineering, scientists seek to directly probe and build... publication at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier... Mary Gearing...

  10. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When...that was open ended... it was actually something bordering on research, rather than 'here’s the protocol,... He first heard about RNAi at his MIT interview during a lecture given by Phil Sharp. After a few rotations... easier for people everywhere…. the nonprofit, sharing ethic that Addgene has behind it is something that... sharing...
Showing: 381 - 390 of 1010 results