...could provide the basis for student projects in introductory labs. Genome projects use computational methods... of MET/Met genes from the pYES2.1 plasmid is controlled by the GAL1 promoter. The cloning procedure adds...absence of methionine. Since the GAL1 promoter controls MET/Met gene expression, complementation should...PMC232622. 6. Winzeler, D.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bnangham...over time: Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1...

... disease. To do so, their T-cells were grown in vitro, their mutations corrected, and the T-cells were... body and thereby correct the mutations using retroviral vectors. However, many diseases require techniques... B, LPL deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark...infect target tissues. Improved specificity through control of gene expression and the selection of specific...CRISPR-AAV vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use...

...better than a nitrocellulose membrane. If you have already run your blot using nitrocellulose, you may decide...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping...instructions for the membrane you are using. Dry nitrocellulose membranes are brittle, so proceed with caution...depend on your blot — antibodies that bind more strongly or that have saturated the blot may need longer...

...systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates. Nature Communications...Peptide-assisted genome editing for primary cells By Susanna Stroik Even with the advances in CRISPR technology over...difficult to genetically edit due to toxicity from electroporation or poor cellular entry with other transfection...viral vector has brain endothelial cell-specific tropism, with slightly reduced efficacy compared to AA9...

...Tsang Fluorescent proteins (FPs) traditionally introduced in Caenorhabditis elegans on extrachromosomal...gene into the target gene’s native locus, but introducing large genetic changes in C. elegans with CRISPR... donors (ssODN). So how can you use CRISPR to introduce large FP genes into C. elegans? By using a split...protein. They used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less than 60 nt...exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells. Its small size offers...

...method can be the absence of suitable controls. The best control for this type of assay is a clonal cell...using a reverse transcriptase mutant in a separate control reaction. This will allow you to quantify the amount...of the qPCR target gene. Developing the proper control line typically requires transducing with a limiting.... 3. Zhang, Bing, et al. "The significance of controlled conditions in lentiviral vector titration and...

... our members took an Arduino microcontroller and a motor controller and made it spin again!  We’ve also...scientists can get involved in modelling, the electronics folks can build a robot worm on top of that model...instance, the centrifuge in the picture had a broken controller and would not spin. It was going to cost far ...far more than it was worth to repair the controller and a new replacement was not possible, but one of our...

...preimplantation embryos that are produced during in vitro fertilization treatments and donated afterwards ... produced Ethical concern High. Embryos are destroyed during ESC isolation. Low Chances of post-transplantation... the reprogramming factors were delivered via retroviral infection [8]. The work was taken forward the...generation of human iPSCs from dermal fibroblasts via retroviral and lentiviral infections respectively [9, 10...cell types. Like ESCs, iPSCs can differentiate in vitro into derivatives of all three germ layers, form ...

... mutated gene. In a Scientific Reports paper introducing the delivery method in 2014, Manuel Goncalves...include their episomal nature and very efficient introduction of DNA into therapeutically relevant, non-transformed...B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used... for cancer therapy. Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan ...adenoviral delivery of CRISPR/Cas9. Journal of Controlled Release 298:128–141 . https://doi.org/10.1016...

...qPCR assays, samples, standard curves, and normal controls must be run in triplicate.  The nice thing about... of DNA in your sample relative to your normal control - you can set up your calculations so that your...so forth. This also serves as a light technical control - an RQ value of 1.5 means you have either made... does not require a standard curve or a normal control, it can take a few hours longer than qPCR to run...AT-rich DNA and by variation in amplicon length. Am J Trop Med Hyg. 2008 Dec;79(6):887-92. PMID: 19052298. ...