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TypeBlog Post...level you can click on your state in this map. Finally, the march is meant to inspire scientists to be...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on...
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Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post... The diversity panel - Equity and inclusion The final and possibly the most important panel of the symposium...quantifying, and preventing this type of failure. Final thoughts The FOR Symposium Boston 2015 shined a ... -
Hot Plasmids: Fall 2025
TypeBlog Post...display the problematic photoswitching upon blue illumination that plagues other red GECIs, so the team could...-translation system (Figure 6). This approach eliminates the need for expensive reagents and equipment... -
Finding Your Science Policy Path
TypeBlog Post...with early career science policy groups, attend webinars, and learn more about the field. Read the CCST...accelerator for some hands-on guidance. Check out my webinar via the NC Science Policy student group SciPolPack... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post... genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous protein tagging...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...variable) Sequence-level data Low-throughput, inappropriate for heterogenous populations, low sensitivity...given the stricter sequence requirement at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al.... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... the annealed cr:tracrRNA is then mixed with recombinant Cas9 protein to form the ribonucleoprotein complex...the promoter region of the non-HDR allele. For N-terminal tagging, specifically searching for error-free... -
Antibodies 101: Flow Cytometry
TypeBlog Post...and fluorescent light sources. Visible light illuminates each cell as it passes through and scatters in...staining is quicker than indirect staining and eliminates potential non-specific staining that may arise... -
Typing CRISPR Systems
TypeBlog Post...The current classification system relies on a combination of sequence similarity, phylogenetic analysis..., & Ke, A. (2024). Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...use, including all information provided in the original reference library, as well as GC%, reverse compliment... that you have specified. Run your screen! Final note I am very grateful to the researchers letting... -
New Coomassie Protocol Video
TypeBlog Post...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies." Hot tip! This protocol can easily... -
Antibodies 101 Series on TikTok
TypeBlog Post...antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations... -
The 12 Days of CRISPR
TypeBlog Post...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR... -
Addgene's 20th Anniversary Party
TypeBlog Post...even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog Post...some time out every once in a while – just read Melina Fan’s post on lunch. Why should our new blog be... -
The Codon Guide to Synthetic Biology
TypeBlog Post...probably the papers and textbooks. I’ve selected “seminal” studies for various categories, including CRISPR... -
Twenty Years of Sharing Science!
TypeBlog Post...community, we've partnered with distributors in China, Korea, Japan, India, and Brazil. We've also created... -
SciComm with the Experts at Science in the News Part 1
TypeBlog Post...communication. Science in the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post... to selecting fluorescent dyes and ligands. Illuminating Choices Paul Heisig, who shared his flow cytometry... -
Introducing Addgene's Microbiology Pages
TypeBlog Post...us about it with our Suggest a Plasmid Form! Finally, if you would like to get the word out about a ...