We narrowed to 954 results for: COMP

...Yeast pGBKCg - Gateway compatible Y2H vector LEU2 Yeast pGADT7-GW - Gateway compatible Y2H vector - AD pGADCg...pGADCg - Gateway compatible Y2H vector BASTA Plant pGTQL1211YN - Gateway compatible BiFC vector (pEarleyGate201... proteins, and more pGBKT7-GW - Gateway compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA...Gentamicin Varies pJL-TRBO - An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression...

...High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-...Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement (MTA), including...Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using...using the materials in the order. After the RSA is complete, Addgene will send you a copy of the MTA, which...

...Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...Fifrefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...-RLuc Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328...deep-learning approaches. Split Luciferase Complementation assays can report on protein-protein interactions... : Constructs for split firefly luciferase complementation assays in plants. Bioluminescence Resonance...

...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy in living cells.... visiting our CRISPR plasmids page . Find a comprehensive list of CRISPR resources by visiting our CRISPR...

...Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous...endogenous genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating... : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with...vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral...transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J...

...parts can be combined to construct complex networks. This is accomplished through the use of assembly standards...suffix sequences that make it easy to combine components for synthetic biology research. Synthetic...meaning that the suffix will need to be reverse complimented for primer design. The restriction enzymes used...

...collection of Golden Gate compatible vectors for bimolecular fluorescence complementation experiments in plants... a library of promoters and purification tags compatible with Golden Gate modular cloning (MoClo) for ...transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques that have been widely...Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions...

...containing 1 mM sodium azide. This buffer is not compatible for use in live cells and will interfere with...secondary antibody. The fluorescence pattern is compared to an untransfected control. Immunohistochemistry...secondary antibody. The tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue...HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the protein size...

...immunogenicity. Viral vectors also vary in the composition of their viral genome, expression of the transgene...one or more plasmids containing the necessary components of the viral genome to assemble the viral capsids...carefully engineered for safety and are replication-incompetent. Specific genes have been mutated or deleted ... self-inactivating, meaning that they can only complete one round of transcription of their viral genome...

...Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them, or skip ahead... Systems Subsequent efforts have improved the components of these systems for better efficiency, sensitivity... transfected or transduced cells express both components in the same relative ratio. Different generations...transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter, and...et al., 2016 (Link opens in a new window) for comparison of Tet-On systems in different applications. ...