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  1. Viral Vectors 101: Pseudotyping

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    ...Protein for Neutralization Assays. Viruses 12:513 . https://doi.org/10.3390/v12050513 Cronin J, Zhang X-Y,...Lentiviral Vectors through Pseudotyping. CGT 5:387–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed...Inactivated by Human Serum. Molecular Therapy 2:218–222 . https://doi.org/10.1006/mthe.2000.0116 Gutierrez-Guerrero...for Research and Gene Therapy. Viruses 12:1016 . https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval...Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089/hgtb.2017.084 Li Q, Liu Q, Huang...for enveloped viruses. Rev Med Virol 28:e1963 . https://doi.org/10.1002/rmv.1963 Mazarakis ND (2001) Rabies...delivery. Human Molecular Genetics 10:2109–2121 . https://doi.org/10.1093/hmg/10.19.2109 Sandrin V, Boson...
  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...methyl-CpG-binding protein 2. Nat Genet 23:185–188 . https://doi.org/10.1038/13810 Amir RE, Zoghbi HY (2000...phenotype-genotype correlations. Am J Med Genet 97:147–152 . https://doi.org/10.1002/1096-8628(200022)97:2<147::aid-ajmg6...Syndrome and Related Disorders. Cell 152:984–996 . https://doi.org/10.1016/j.cell.2013.01.038 Guy J, Gan ...Model of Rett Syndrome. Science 315:1143–1147 . https://doi.org/10.1126/science.1138389 Le TTH, Tran NT...Human-Induced Pluripotent Stem Cells. Front Genet 10: . https://doi.org/10.3389/fgene.2019.00625 McGraw CM, Samaco... Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126/science.1206593 Powers S, Miranda...MeCP2 null (S51.002). Neurology 92 (15 Supplement) . http://n.neurology.org/content/92/15_Supplement/S51.002...
  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...targeting with CRISPR–Cas13. Nature 550:280–284 . https://doi.org/10.1038/nature24049 Find plasmids from...high-fidelity genome-editing platform. Genome Biol 19: . https://doi.org/10.1186/s13059-018-1591-1 Cox DBT, Gootenberg...editing with CRISPR-Cas13. Science 358:1019–1027 . https://doi.org/10.1126/science.aaq0180 Find plasmids...endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609 Find plasmids from this...derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038/ncomms14500 Find plasmids from...altered PAM specificities. Nature 523:481–485 . https://doi.org/10.1038/nature14592 Find plasmids from...nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620 Find plasmids from this...
  4. Generating Mouse Models Using CRISPR/Cas9

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    ...Using CRISPR/Cas Systems. Science 339:819–823 . https://doi.org/10.1126/science.1231143 Doench JG, Hartenian...gene inactivation. Nat Biotechnol 32:1262–1267 . https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi...Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002/0471142905.hg1508s88 Leidy-Davis...CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-018-33408-9 Mizuno N, Mizutani...Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci.2018.10.030 Qin W, Kutny...Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002/9780470942390.mo150178 Qin W, ...Electroporation of Nuclease. Genetics 200:423–430 . https://doi.org/10.1534/genetics.115.176594 Yang H, Wang...
  5. Viral Vectors 101: Producing Your rAAV

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    .... Human Gene Therapy Methods, 23(1), Article 1. https://doi.org/10.1089/hgtb.2011.034 Benyamini, B., Esbin...Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/10.1101/2023.03.20.533580 Goodwin, M. ... Molecular Neurodegeneration, 15(1), Article 1. https://doi.org/10.1186/s13024-020-00361-z Grimm, D., ...recombinant AAV-2. Gene Therapy, 6(7), Article 7. https://doi.org/10.1038/sj.gt.3300946 Howard, D. B., &.... Human Gene Therapy Methods, 28(1), Article 1. https://doi.org/10.1089/hgtb.2016.180 Korte, J., Mienert.... Human Gene Therapy, 32(13–14), Article 13–14. https://doi.org/10.1089/hum.2020.120 Lock, M., Alvira,.... Human Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089/hgtb.2013.131 Lock, M., McGorray...
  6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    ... Integrated in Parallel. Cell, 154(4), 914–927. https://doi.org/10.1016/J.CELL.2013.07.018 Anzalone, A...breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J.,...manipulation. Nature Reviews Genetics, 24(3), Article 3. https://doi.org/10.1038/s41576-022-00541-1 Kim, H. K.,...performance. Science Advances, 5(11), eaax9249. https://doi.org/10.1126/sciadv.aax9249 Koeppel, J., Weller... determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023-01678-y Li, X., Chen...epigenetic manipulation of prime editing. Cell. https://doi.org/10.1016/j.cell.2024.03.020 Mathis, N.,... learning. Nature Biotechnology, 41, 1151–1159. https://doi.org/10.1038/s41587-022-01613-7 Mathis, N.,...
  7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...methylated somatic enhancers. Nat Genet 52, 819–827. https://doi.org/10.1038/s41588-020-0639-9.  Feinberg A...from their normal counterparts. Nature 301, 89–92. https://doi.org/10.1038/301089a0. Galonska C, Charlton...methyltransferase footprints. Nat Commun 9, 597. https://doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich...activity and function in development. eLife 7:e34870. https://doi.org/10.7554/eLife.34870.  Kaas GA, Zhong C... and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita ...catalytic domain fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/10.1038/nbt.3658. Sapozhnikov DM, Szyf...methyltransferase with CRISPR/dCas9. Nat Commun 12, 5711. https://doi.org/10.1038/s41467-021-25991-9. Spruijt CG...
  8. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...enhanced multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554/elife.43322 Baker M (2015) Reproducibility...Blame it on the antibodies. Nature 521:274–276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012...preclinical cancer research. Nature 483:531–533 . https://doi.org/10.1038/483531a Bradbury A, Plückthun ...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun...validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein... functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Crosnier...mouse monoclonal antibodies. BMC Biology 8:76 . https://doi.org/10.1186/1741-7007-8-76 Greenfield, E.A...
  9. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
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    ...CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Chavez A, Scheiman ...transcriptional programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M,...activators in multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham...engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak...transcription factors. Nat Methods 10:973–976 . https://doi.org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert...Control of Gene Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik...system differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-019-54179-x Tanenbaum ME...
  10. Viral Vectors 101: The Retroviral Lifecycle

    Type
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    ...retroviruses. Microbiological Reviews, 56(3), 375–394. https://doi.org/10.1128/MR.56.3.375-394.1992. PubMed PMID...Current State and Perspectives. Viruses, 13(7), 1288. https://doi.org/10.3390/v13071288. PMID: 34372494. Prussin...Environmental Science & Technology Letters, 2(4), 84. https://doi.org/10.1021/ACS.ESTLETT.5B00050. PubMed PMID...of the United States of America, 54(1), 137–144. https://doi.org/10.1073/PNAS.54.1.137. PubMed PMID: 4285422...precursor protein. The EMBO Journal, 16(16), 5113–5122. https://doi.org/10.1093/EMBOJ/16.16.5113. PubMed PMID:...virus DNAs. Journal of Virology, 14(5), 1132–1141. https://doi.org/10.1128/JVI.14.5.1132-1141.1974. PubMed.... Journal of Molecular Biology, 79(1), 163–183. https://doi.org/10.1016/0022-2836(73)90277-5. PubMed PMID...
Showing: 31 - 40 of 246 results