We narrowed to 911 results for: TRO

...always include proper controls like uncut DNA and the proper ladder. These controls help you properly visualize...sequencing the chromatogram is your visual control. And, like all controls, missing out is a big mistake. Chromatograms...Chromatograms come to the rescue for analyzing and troubleshooting your DNA sequencing results Above (top right...repeated. Guidelines to help with DNA sequencing troubleshooting and analysis 1. You can use any of the following...70-75 of the read (see image below), so I would strongly recommend using a silica spin column. They can...most out of your DNA sequencing results and to troubleshoot any problems that come up. Good luck analyzing...

... in extracurricular activities (EA). EAs can: Introduce new perspectives in science and engineering Looking...that your project is an innovative solution with a strong impact, or a journalist does not understand the...

...Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use electroporation to ensure...library, Addgene usually does four to eight electroporation reactions using a total of ~400ng of library...

...spatial control. Optogenetics uses genetically encoded tools, such as microbial opsins, to control cellular...genomic loci Photochemical Control Hemphill et al., 2015   Nitrobenzyl-caged lysine Cas9 K866PCK mutant...tools allow scientists to use light to externally control the location, timing, and reversibility of the ...process. Read on to learn about the various light-controlled CRISPR tools available to researchers - some ...published in early 2015 focused on using light to control transcription. Two separate labs, Moritoshi Sato...gene expression while imparting spatiotemporal control, reversibility, and repeatability. The system developed...expression of the human ASCL1 gene could be spatially controlled in a reversible and repeatable manner. With their...

...loading controls are equally strong, so the normalized value simply reports which POI band is stronger. The...loading control is not perturbed by the experiment The loading control is supposed to control for, well...western blots benefit from a positive control — a loading control — that you know is present in every lane...transfer. Figure 1: A loading control is a type of positive control necessary to demonstrate a POI’s...loading control: the intensity of each POI band is divided by the intensity of the loading control in the...with loading controls. Under each lane, the fraction of the POI band over the loading control is shown. ...loading control. First, let’s explore why these assumptions are key to using loading controls accurately...

...choice for targeting astroglia. It has additionally been reported to have tropism for liver, vascular ... It can transduce neurons for retrograde labeling, as well as astrocytes in the CNS (Han et al., 2023)...following table summarizes tissue tropism as we currently understand it. Tropism depends on many variables, ...Table 3: AAV tissue tropism. To target... Try using... CNS AAV1, AAV2, AAV2-retro (AAVrg), AAV5, AAV8...enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction...the opposite: AAV serotypes with varying tissue tropism, allowing them to target particular cell types....commonly used serotypes in this post. However, tissue tropism varies both between species and even between strains...

...The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted...flexibility to pseudotype γ-retrovirus using different envelopes to modify tropism. Briefly, different envelope...either an ecotropic envelope, Phoenix-ECO, (for infection of mouse and rat cells), or an amphotropic envelope...prototypical gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered...MoMLV and MSCV for γ-retrovirus; HIV for lentivirus). Additionally, γ-retroviruses can only transduce dividing... Are γ-retroviruses self-inactivating (SIN)? They can be. Plasmids that encode SIN γ-retrovirus lack most...blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in Research...

...and developed the SciNote Electronic Lab Notebook. Implementing an electronic lab notebook starts with ...should have functionalities such as: electronic signatures, electronic witnessing and audit trails. How ...write things down when doing scientific research? Electronic lab notebooks and researcher's needs The familiar...traceability and reproducibility of research. Electronic lab notebooks (ELNs) are here to make lab work...This includes financial outlay, staff hours, troubleshooting, and the fact that long-term use is likely ...traceable. However, switching from paper to an electronic lab notebook requires time and knowledge. In ...recent article, we translated our knowledge of electronic lab notebooks into 9 questions that will help...

...highest quality, we undertake a rigorous quality control process. In 2016, we launched our viral service...our scientists perform all the standard quality control assays for AAVs. These steps include measuring ...Addgene is also conducting more rigorous quality control, including next generation sequencing (NGS) of ...VGS). Why should you care about viral quality control? AAV vectors are produced by scientists - not robots...Lecomte et al., 2015). This higher level of quality control is recommended but not yet required for clinical-grade...addgene.org to answer your questions about our quality control process! The images in this blog post were created...2011) Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses...

...the go-to method is to use spectrophotometry. Microvolume spectrophotometers make it quick and easy to ... jumping in — in the world of microvolume spectrophotometers, the Thermo Scientific NanoDrop is one of...details might vary.  How it works — briefly Spectrophotometers measure how light travels through a sample...contaminants, some of which are easier to detect by spectrophotometry than others. The ones most likely to impact...making it more difficult to detect. A particularly strong absorber at 230 nm is guanidine thiocyanate (used...Phenol is a unique case, as pure phenol absorbs strongly at ~270 nm and exhibits A260/280 and A260/230 ...distinguishing between the two can be difficult by spectrophotometry. RNA tends to yield a higher A260/280 and ...