We narrowed to 45 results for: golden gate assembly

...and more. Also contains pre-assembled genetic circuits such as logic gates and higher level gene networks... Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio...James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff Hasty Dieter Jahn Alfonso Jaramillo...Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit...Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium...

...Technology Pros Cons References Golden Gate Type II restriction enzymes Can assemble up to 10 modules in a single...2008 Gibson Cloning Gibson assembly method Single reaction, can assemble large DNA sequences, no restriction...same multiple cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion...upon ligation. As a result, once two modules are assembled next to each other, a third module cannot be placed...high-throughput cloning strategies available for assembling these modular types of constructs, and different...restriction enzyme-based). Well suited for the modular assembly of highly similar or repetitive sequences. Intermediate...called “cassettes”) can be readily reused in the assembly of any later construct (i.e.,functional cassettes...

...al., 2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the...and Dinneny, J. R. (2013) ‘A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology...Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping and...transformant with a single insertion because it will segregate 3:1 in the T2 generation. You also have no control...genotype in T1 than T2. Your transgene should not segregate in T1, but it probably will in T2. Laura Lee...PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation...

...collection of reporter plasmids is amenable to Golden Gate assembly for turning the single-input reporters into... into multi-input cell classifiers in one assembly step. Find the miRNA reporter collection at Addgene...

...regulatory sequences ready to be assembled with other elements using golden gate cloning. This toolkit is designed...

...Open Enzyme parts have C/E overhangs for Golden Gate assembly. Plasmids Explore the collection and order... Plasmids Jennifer Molloy Lab Plasmids Blog: Golden Gate Cloning...

... Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9...

...Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden Gate Cloning Read all of our Plasmid Cloning Posts..., the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several...recombinant plasmid to propagate. Any cells that contained non-recombinant vectors (re-ligated empty vectors, ...ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing...focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what ...expressed the ccdB gene and were therefore unable to propagate because CcdA wasn’t available to counteract the... to thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially...

...promoters and purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . ...Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible vectors for bimolecular fluorescence... in plants. The Zinc Finger Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain...Collection page to find plasmids and kits for efficient assembly of TALEN constructs. The Zinc Finger Consortium...

...to quickly assemble vectors and test different ideas. We are a huge fan of the Golden Gate based MoClo...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...