1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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2. Five Popular Model Organisms, Part 2

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3. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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4. How to Design Your gRNA for CRISPR Genome Editing

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5. Finding Your Science Policy Path

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6. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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7. Overcoming the AAV Size Limitation for CRISPR Delivery

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8. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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9. CRISPR 101: Multiplex Expression of gRNAs

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10. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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11. Optogenetics + CRISPR, Using Light to Control Genome Editing

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12. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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15. Hot Plasmids - August 2020

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16. Stepping into a Virtual Internship: My Experience at Addgene

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17. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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18. Reagent Repositories Are Speeding up Science During the Pandemic

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19. CRISPR 101: RNA Editing with Cas13

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20. Capturing a Moment with Marc Zimmer’s “The State of Science”

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21. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

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22. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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23. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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24. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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25. How to Prepare for an Industry Interview

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26. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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27. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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28. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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29. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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30. CRISPR 101: Epigenetics and Editing the Epigenome

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31. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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32. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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33. Plasmid Preps: Different Purity, Different Quantities, Different Uses

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34. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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35. What's the Best Way to Elute and Store Your Plasmid DNA?

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36. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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37. New and Upcoming Viral Vectors - May 2020

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38. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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39. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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40. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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