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We narrowed to 345 results for: Ada

Showing: 121 - 160 of 345 results

  1. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ..., the ability to embrace serendipity fosters adaptability, a crucial skill for a successful research career...research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries...

  2. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...antibody-dependent cellular phagocytosis (ADCP). Figure adapted from van Erp, et al., 2019.    Antibody-dependent...Song, WC. Complement and its role in innate and adaptive immune responses. Cell Res 20, 34–50 (2010). https...

  4. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...recombinase-based state machines and how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-... understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert...

  5. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor multiple ...- Overview of the neural and gut fiber system (adapted from Sahasrabudhe et al., 2023).   To develop...

  6. What the HEK?

    Type
    Blog Post
    ...adherent monolayer, though derivatives have been adapted for growth in suspension. Of the many derivatives... allows for large scalability, and can also be adapted for serum-free growth, which significantly reduces...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...tool for enrichment of ABE-targeted cells By Ellie Adams Single base modifications through ABE or CBE's have... from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian cell lines. ...

  8. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. In certain instances,...mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    ...nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022.   Find CRISPR...temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods ...

  14. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Arabia -- as well as closer to home in the US and Canada. In fact, we received so many requests (175 and...imagine, it’s also been quite the administrative headache receiving constant email requests and tracking...

  15. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids on a schedule... your work schedule as possible so that you can adapt to your kids' needs. Plan ahead. Think about and...

  16. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...with synthetic biology lab kits and lesson plans adapted from her own MIT curriculum. Drawing on current... BioBuilder book chapter and an illustrated, downloadable classroom poster — with both of these resources...

  18. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...intra-molecular annealing. Figure and legend from Okada, 2013.   scAAV If your gene of interest is truly...PMID: 20649475. PubMed Central PMCID: PMC2957237. Okada, T. (2013). Efficient AAV Vector Production System...

  21. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting the appropriate...single gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden...

  23. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016. Until recently...

  25. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative ...Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012;337(6096):816...

  26. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...routinely in biotechnology to transform plant cells. Degradative plasmids: microbial hardiness There are also ...organisms. To conserve energy, these specialized degradative capabilities are often tightly regulated to be...

  27. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...longevity in eukaryotes and promote transcript degradation in prokaryotes.  Prokaryotic termination   Prokaryotic...important for stability of the mRNA, protection from degradation, and is integral to the nuclear export and translation...

  28. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated...

  29. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...References and Resources References Braun, C. J., Adames, A. C., Saur, D., & Rad, R. (2022). Tutorial: design...Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J., & Kampmann...

  30. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools, they’re optimistic...optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing science...

  31. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...the kinetic response time from signal input to readable output, which generally exceeds that of the VSD...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

  32. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... co-purify with your plasmid DNA and lead to degradation of your prep. Many plasmid prep kits recommend...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

  34. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...blog posts and additional resources into one downloadable PDF that you can save on your desktop. The Plasmids...

  36. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated..., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C. M., Leib, R. D., Cirolia, G., Thomas, D., Stamnes...

  37. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...resistance to both carb and amp, due to their common degradation mechanism. How to choose between the two? Carb...high pH environments and is less susceptible to degradation in agar plates. This prevents satellite colonies...

  38. An Introduction to Adenovirus

    Type
    Blog Post
    ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes..., J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., Rizzo, K., Bertumen, J. B., Morales...

  39. DNA Purification Without a Kit

    Type
    Blog Post
    ...or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published...100 bp to 48 kb with high yields and without degradation during the extraction procedure. Additionally...
Showing: 121 - 160 of 345 results