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We narrowed to 801 results for: MAK

Showing: 121 - 160 of 801 results

  1. Hot Plasmids - August 2020

    Type
    Blog Post
    ...NB) were generated in pMAK:  pMAK461(NBαRabitb-IgG-Fc, Addgene #140699) pMAK462 (NBαMouse/Rat-IgG1-Fc...#140700) pMAK463 (NBαMouse-IgK, Addgene #140701) pMAK464 (NBαEGFP, Addgene #140702) The pMAK461-64 plasmids...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...which alleviates the inclusion body problem and makes nanobody production in E.coli as simple as following...miniprep protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector...used for in vivo biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by ...

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... paper into an electronic environment and hence making it searchable and shareable, but some ELNs provide...community were to: (1) prevent vendor lock in by making it easy to get data out of the ELN; and (2) facilitate...re-used frequently in the group’s research – it makes no sense and is not practical to move all this data...references the record of research is incomplete, making it difficult or impossible to reproduce. As the...external data sources, they also need to continue to make use of other tools they use in research. This includes...sharing and reproducibility ecosystem protocols.io makes possible. Connectivity to open source data repositories...have been launched and are now a main vehicle for making research data available for public access, review...

  3. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...receive such a call, make sure to get that person’s contact information and again, make a personal connection... the hundreds of others that did the same thing? Make an effort to meet people. Attend smaller talks within... CV or resume). If you receive a card in return, make sure to follow up with a professional “thank you...company. It does, however, offer you an opportunity to make a personal connection; the importance of which will...there, so that you can tailor your CV accordingly. Make it stand out with key words and by putting your ...’ll find a variety of listings. Post your CV and make it visible, which will sometimes result in a recruiter... a traditional training program, and you hope to make a career in academia, you are likely in good shape...

  4. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...goals. These tools remind us of our daily tasks, make our days more effective, and our schedules more ...research. Electronic lab notebooks (ELNs) are here to make lab work much more reproducible, convenient and ...required every once in a while, so it is good to make sure you’ll be able to do it when needed. How many.... If these are important in your lab, you should make sure your ELN allows you to assign specific permissions...ownership should be yours (your institution’s), so make sure you don’t use ELN providers that take any ownership...set for every member of your team. Do you need to make sure your research can be patented? I.e. is automated...difficult to figure out all the ways a given ELN can make your lab life easier. If you can talk to an ELN ...

  5. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Coaching can be an excellent tactic for scientists making a career shift or who wish to improve their current... committed to. Finally, hiring a paid coach will make you take the experience more seriously and I have...good ones take this responsibility seriously. As I make my rounds speaking at research institutions and ... Sometimes only a few hours of coaching time can make an enormous difference so don’t assume that this... with. Talk to the coach about their “style” and make sure this is a style that will help you. A good ...good coach usually will be a clear communicator and make direct suggestions for improvement and change. ...launched an online career coaching program that might make her coaching more affordable for you. There is also...

  6. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ... as well as the instructions on how to make your own, will make it easier for you to use genomic tagging...alleninstitute.org. A classic challenge in cell biology is making sure that what we observe through the microscope...and result in a ubiquitous background signal that makes it hard to visualize the precise location of the...cellular structures, we provide guidance on how to make your own gene-tagging constructs below. Read on ...process following the double strand break CRISPR/Cas9 makes at the target site. The whole segment—a GFP tag ...cells. As a precautionary measure, it’s also good to make a few single base pair mutations in crRNA target...and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and introducing the tag...

  7. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...nice if you can afford them). I am talking about making sure the audience is interested, educated and talking...with room for fortuitous improvisation. You want to make it look effortless to the audience, but the organizers...moderator should have a well-planned approach to making the event interesting and engaging. Long before...funny, provocative or interesting stories that will make for a fun discussion. People are much better at ...interesting questions and topics in advance is key. Make sure all your panelists have the correct date, location...as it approaches. Room set-up and logistics To make a really lively discussion, I strongly suggest asking...colleagues to events for this purpose. If possible, make sure the panelists are prepared to stay around to...

  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... in the world knows it—to be the first person to make a discovery. I can’t tell anyone else what the “...cases, don’t enjoy, is worthwhile and can help us make decisions about what we want to do in the future...only demonstrates initiative and drive, but also makes for an experience more tailored to personal interests...professors about their research, and learn about what makes them excited to do what they do. I would also highly...eggs in one basket. There are many factors that can make or break a lab experience. Previous Addgene blogs...want to dedicate your time and energy to. How can I make the most of my experience in the lab? Ask your questions—especially... I’ve been afraid to ask “dumb questions” and to make mistakes. Ultimately, though, scientists are all...

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...produced in the lab. The first decision you must make is which system you will use, or more specifically...specifically, the type of cells that will be used to make the virus. Viruses can be produced in many different... by the specific type of virus you are trying to make. For example, bacteriophages will need to be produced...methods, understanding the differences can help you make an informed decision. Mammalian cells Mammalian ... they contain some of the necessary machinery to make these viruses. The cells are also easy to cultivate...mammalian cells. However, it is much more common to make lentiviruses and AAV in-house. High-titer adenoviruses... that specifically infect insects. This is what makes insect cells a popular choice for protein production...

  10. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...lead to hurt feelings, low morale, poor decision making, and stalled projects. You want to identify and...experiments to address a reviewer’s concerns, you are making your hypothesis stronger. When you share data at...re having--that conflict you are resolving--will make you a better scientist. And some amount of conflict...miscommunications in the future. Assumptions: Making incorrect assumptions can be a direct cause or ...or consequence of lapsed communication. Making some assumptions about how something should be communicated...know that a person or team should be involved in making a decision and you see no record of their input...in question to a specific person or team. What Makes You a Good Conflict Resolver? The first and foremost...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...much for how bright you can make a reaction but for how sensitive you can make a reaction. NLuc requires...proteins to make better reporters and the second couples NLuc to fluorescent proteins to make better biosensors...interesting construct you wished to use, you could either make it yourself or you could “clone by phone”. One of...tactics for getting the plasmids he wanted. Addgene makes it so much easier to share your constructs from ... however NLuc’s emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution...the fluorescent donor for a bioluminescent donor, making BRET probes. Here the bioluminescent donor is unaffected... fluorescent proteins has allowed researchers to make innovative tools not possible with other luciferases...

  12. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...genome is 1 in every 256 base-pairs of DNA sequence, making it very useful for genetic engineering approaches... and gene editing. How does the piggyBac™ system make it easier to generate transgenic cell lines? One...transgenic cell lines. The traditional method of making stable cell lines is to transfect, select, and ...negative impacts on fitness. One consideration when making a transgenic cell line with the piggyBac™ system...However, several challenges still remain, such as making gene edits completely seamless (i.e., so no other...piggyBac™ transposase (PBx), which contains mutations making it competent for excision, but defective for integration...larger piggyBac™ donor vector, it is possible to make the ITR and selection cassette disrupt the target...

  13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ... As a member of the Quality Control team, I help make sure the plasmids that go out our doors are correct...assistant in a lab at the university that I went to. Making that switch from being a grad student to an employee...she didn’t want to take it. “I realized if I don’t make it to med school it’s not the end of my life, even...programs. I thought, ‘Let me pursue this, let me make a slight change so I can have flexibility and be...is incredibly helpful to me to know that you can make big changes in your career path and still find comfort...never work a day in her life. She said, “it now makes sense because I love working in the lab and being...people and for the people, feeding the people, and making them get outside of their comfort zones. I came...

  14. 6 Tips for Grant Writing

    Type
    Blog Post
    ... to read it, give it back to you, and for you to make the relevant alterations before submitting - handing...otherwise why would you be interested in it?) so make sure that comes across. Check out some in-depth ...agencies want value for their money so you need to make it clear that you will be doing your research in...routinely isn’t common to non-scientists and may make your perfectly reasonable paragraph unintelligible... about what I hope to discover. This section may make the difference in whether you get funding or not...Improve Your Science Communication Skills 5. Practice makes perfect, apply for small grants too There are actually... awarded money to carry out research, be sure to make it clear what the outcome of the work was. Ideally...

  15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...to Ireland and take a chance as entrepreneurs. To make things more interesting, we are working in a new.... Being unable to publish or attend conferences makes it very challenging to compete in today’s tough ... a lot of time talking about what we could do to make ourselves marketable to the wider world and occasionally...Keira’s an optimist, I’m a realist, and together we make a pretty good team. We have confidence that we can...the lines of “I hate GMOs, but what you are doing makes sense. I can support that.” That was interesting...technology we will need, and the business training to make the most of it. It has also allowed us to really... we enjoy and see a future in. And we decided to make education and outreach a cornerstone of our business...

  16. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...this make things more manageable, but it also keeps stock solutions from getting contaminated. Make sure...minutes before you start any tissue culture work and make sure that clean air is flowing in. The vent for ...not be used as storage cabinets. Clutter not only makes it difficult to clean your workspace properly, but...disrupt the laminar air flow around your work area. Make sure to only bring required materials into the hood...stay out too long While you’re preparing things, make sure your cells don’t stay out too long. Cells usually...indicates that the cells have grown enough, are making their surrounding media acidic through the secretion...all but one in liquid nitrogen. The frozen vials make up your master stock, while you can use the remaining...

  17. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...more easily identify what to delegate and to whom (make sure that person has time for it!). Then schedule...not a clear deadline. Finally check-in regularly, make yourself available, and encourage questions. Unless...opportunities. I still need to check myself sometimes to make sure I am delegating well, but it does get easier...grateful for the feedback, but at least you can start making changes for the best. Positive feedback is equally...experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members need to trust...to encourage them to speak their minds. They will make mistakes and you will take responsibility (within... and help you grow in your role. The latter will make you feel discouraged and not valued which will undoubtedly...

  18. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...you use depends on your preferences. Either way, make sure to test your colony PCR primers before using...insert or if the insert is in your plasmid Have to make a new primer pair for each insert Backbone-Specific...plasmid Great for blunt end cloning You have to make a new primer pair for each insert since primers ...positive ones. Regardless of which method you choose, make sure to use the appropriate antibiotic for selection...polymerase is sufficient. Controls: Controls can make or break an experiment. The best controls for a ...products on an agarose gel to determine their size. Make sure to use an appropriate molecular weight standard...doesn’t mean there aren’t mutations in your insert. Make sure to submit multiple positive clones for sequencing...

  19. Promoters

    Type
    Guide
    ...Promoter Regions There are three main portions that make up a promoter: core promoter, proximal promoter,...

  20. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...manager quickly make the connection between your skills and the needs of the job. Make it clear that you...successful, reproducible assay. That information can make you a much stronger candidate!  Additional uses ...cover letter to explain why your transferable skills make you a good candidate for the position. You can also...could talk about how your experience with qPCR would make it easy to master ddPCR.  Finally, it’s a good idea...highlight what specific skills, qualities, or interests make you a good candidate for the job. The body of the...should be one to three paragraphs. This is where you make the case for why you are a good candidate, explain...start from a standard version, but take some time to make it fit the job you’re applying for. The cover letter...

  21. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...philosophy Customer service can be a hassle. You finally make it through a 20-step phone tree, only to be left... customer support with self service We strive to make our support team available whenever you need them...not have the chance to stop what you’re doing and make a phone call or write out an email. You need help...resources, we will continually update the Help Center to make sure that, whatever your support needs are, we’ve...ll navigate to articles answering your questions making it easier than ever to get the support you need... regular site, www.addgene.org. We’re excited to make these new features available to the Addgene community...valuable feedback. This is Addgene after all, and making your research easier always comes first. Resources...

  22. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...produced in abundance, making them useful for in vivo studies. These attributes make frog eggs some of the...physiological, or developmental characteristics that make them ideal for studying in a laboratory environment...Additionally, its status as a Biosafety Level 1 microbe makes it a perfect candidate for introductory teaching...Iannaccone et al., 2009). Rats are larger than mice making them more useful for certain studies that involve...axolotl has several attractive characteristics that make them an ideal model organism; they are relatively...genetics and developmental patterns are conserved, making them great models for human disease research. One... alike.  Arabidopsis has many of the traits that make an ideal model organism: quick development, a large...

  23. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...’s critical to make these decisions in the following order of importance: 1. First make decisions based... Then make decisions based on financial values. All too often, we see early investigators making unnecessary...step by step instructions on creating a budget, making decisions based on that budget, and negotiating...science will define your success. Therefore, it only makes sense to reverse engineer your budgetary needs from...you can eliminate from your budget. Every cut you make signifies that you've made a conscious effort to...dependent on a pathology microtome/cryostat, then making a careful decision on the condition and availability...

  24. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ... studies, making a meta analysis available to the community on ReFigure. ReFigure also makes it easier...during this period occurs infrequently and rarely makes it into the small selection of data found in full-length...early career researchers, we wondered how we could make the insights gained during this time more visible... on science. ReFigure saves time, knowledge and makes your insights discoverable. Watch this quick video...cell lines/model systems ReFigure allows you to make meaningful collections from a large section of open...paper they’re reading has been linked in a ReFigure making it easy for others to discover your insights on...

  25. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...and numerous experimental design decisions to make, it makes sense that scientists, many of whom are venturing... used to select your genomic target, you need to make sure the NGG immediately follows your target on ...and bi-allelic cells. Single-allelic cells usually make up the majority in culture unless the targeting ...target in the HR template, you usually would need to make mutations of the HR template to avoid this donor...or 'NGA', or 'NGC' (if it's within coding region make sure it's a silent mutation). Q8: Is a protospacer...targeted and degraded by the Cas9. It is possible to make a silent mutation or avoid putting in the full target...choosing target sites that span the knock-in gene. For making mutations, one good way is to mutate the PAM 'NGG...

  26. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...culture towards a more generous and rewarding way of making science.   How we started Crowdfight-COVID19...Confirm the expert’s suitability and availability; 3) Make the contact to start a direct collaboration between...realization that there is a great untapped potential to make science more efficient, open, and collaborative....collaborative. We now want to use this potential to make a difference not just against COVID-19, but also in other...These are transient partnerships where a researcher makes a small but crucial contribution to someone else...We are a community of researchers who believe in making science less of an individual effort and more of...bono. If this sounds appealing, join us and help make science a better place. You will receive opportunities...

  27. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well...Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method...essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for ...Yesbolatova et al., 2019) using CRISPR. This toolkit makes it easy to tag your protein of interest to create...create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor...such as a distal promoter and an enhancer. What makes LADL unique from previous iterations is the addition...

  28. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...rushing, you’re much more likely to make a mistake. When you make a mistake, you’re more likely to be ...and the same goes for your workflow. Next time you make a mistake, instead of silently vowing never to do... physical copy of the protocol when you work and make a checkmark next to each step as you complete it...experimental steps are worth doing, and doing well, so make sure you’re taking time to do them carefully and...reach for spreadsheets, printouts, and even label makers to keep things legible. It is faster to spend more...however, many ways for you to reduce the chances of making an error. Try a variety of things, including (and...

  29. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...Alzheimer's - a connection I wouldn’t have thought to make (even if science writer Ed Yong’s book “I Contain... skills you’ve developed in your current work to make new insights in neuroscience. Even if you're currently...expertise and can be difficult for individual labs to make efficiently. For select viral vectors in the repository...repository, you can instead have Addgene make the virus for you. Neuroscientists are particularly interested...experiments. This helped reinforce our goals of making more AAVs and educational content to help researchers...for future experiments and to find tools that will make your transition into the field much easier! If there... suggest a plasmid to us, request that we start making the AAV, or help us produce new educational content...

  30. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...as a great first-time mentoring experience. They make mentoring less mysterious and train the participants...Provides a good mechanism for accountability that makes mentoring effective Goal setting is easier with ...example, a group of people who are all interested in making a transition. Schedule your first meeting and get...regularly no matter what, even if one person can’t make it. One of the biggest group mentoring predictors...breakfast once a month. All participants must commit to making the meetings a scheduling priority (and last minute... Set concrete goals with input from the group to make the plan better. Track your goal progress as a group...the series to get more advice on mentoring: What Makes a Good Mentor? Choosing a Good Mentor for Scientists...

  31. Finding Your Science Policy Path

    Type
    Blog Post
    ...during your current job and note what makes your heart sing, what makes you pull your hair out, your successes...opportunities to pivot and grow along the way. That’s what makes it so wonderful. The most challenging part of jumping... in a government setting: drafting legislation, making rules, and implementing the policy within the jurisdiction...career or a part-time passion, there are many ways to make it work for your time and interests.  Action Items... healthcare systems so I could understand how to make new treatments and technologies accessible to patients...healthcare system? Defining your why is what will make you a competitive candidate for any science policy...your interest to future positions, but will also make your transition from academia to the policy world...

  32. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...of the Cas9 nuclease (PDB ID: 4OO8 and 4UN3), Slaymaker et al. hypothesized that Cas9 cutting efficiency...enough to keep off-target sites from being cut. Slaymaker et al reasoned that, if they decreased the positive...the electropositivity of the HNH/RuvC groove, Slaymaker et al. made a variety of alanine substitutions...off-target effects genome-wide. Further work by Slaymaker et al. showed that these mutants are not toxic...SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian cells and may decrease...look forward to seeing how the research community makes use of these tools and are excited to see continued...improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered Cas9 nucleases...

  33. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...technology and synthetic biology, the tools he’s making available through Addgene, and where all of it ...CRISPR is so orthogonal and programmable that you can make a very large number of regulators. That basically...have the solution. Our paper shows we can easily make orthogonal variation. The first one worked in each...off-target interactions. That’s relevant if you want to make sure you don’t target anywhere else, but for synthetic... been putting together very large toolboxes and making them available. We’ll also be submitting parts ...you want. The last few years, we’ve really been making tons of parts and characterizing the software to...sophisticated as those that living cells and animals make. That will be an outcome of genetic engineering....

  34. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ..., need to stay active and involved if we wish to make positive change. Below I present perspectives on...science happens when scientists have 1) the courage to make mistakes and 2) the integrity to recognize these...scientific evidence. We need representatives and policymakers who not only believe in the separation of church...-two-new-sciences). This is the approach policy makers take in abusing climate science skepticism to promote...averting a potential climate disaster. However, policy makers use these two fingers of skepticism to defend an...world. Justin Sherman is a Scientific Instrument Maker at Intellectual Ventures Laboratory. Vancouver, ...ongoing and difficult job. I urge other marchers to make this their first step, and continue this work by...

  35. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...few things you can do to make the experience less stressful. First, you can make arrangements with those...company’s area of expertise. This has potential to make your audience really sit up and pay attention. Your...demonstrate technical and transferable skills that make you qualified for the position. Highlight the outcomes...improving a process or procedure, solving a problem, or making a key decision. Even deciding to stop pursuing ...4.5 hours, which was a lot of extraversion for me. Make sure to get enough sleep the night before. Have ... look professional  First impressions matter, so make sure yours counts. Dress professionally but still...

  36. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...tips on how to make a successful event at your company. The point of this post isn’t to make you all jealous...are busy with many other work responsibilities, we make team building activities a priority and have organized...though we are hiring!) but to encourage everyone to make an effort to build relationships with their colleagues... it simple This may be an obvious one, but don’t make things too complicated for yourself (especially ...for help My grandma used the old adage “many hands make light work” all the time. She was a wise lady for...will save you time and sanity!    10. Communicate Make sure people know that there is something fun happening...

  37. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov...://doi.org/10.1261/rna.054098.115 Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...

  38. Five Popular Model Organisms

    Type
    Blog Post
    .... Scientists can make a new line of flies in only 6 weeks compared to months to make a transgenic mouse...in the lab, and have short generation times that make it easy to study the effects of genetic manipulations... be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila melanogaster...genetic material (Jennings, 2011).   The fruit fly makes a great model organism because they are easy and...C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments over multiple...60% of human genes have an ortholog in C. elegans making it ideal to study basic molecular biology processes...

  39. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...between different projects, you might not be able to make any reasonable progress on any one of them. That...another. Monitoring task progress. For this, I always make sure I have explicit goals in mind for the endpoints...everything in a Word or google doc that I can comment and make notes on as I progress. I’ve also recently started...world, procrastination can easily prevent you from making progress on a task. For instance, even if I had...over the activation energy barrier that comes with making ANY progress, the task will likely be much easier...easier. Having a formal plan for your day will make this easier - if you know you have dinner plans at 6...pm. Hopefully you’ll find this post useful for making your days, experiments, and projects more manageable...

  40. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ... grow quickly and make more of the plasmids as they grow, scientists can easily make large amounts of ...guidance to understand the specific components that make up a plasmid and why each is important. Our “Plasmids...ability of plasmids to self-replicate within a cell make them attractive tools for the life scientist or ...particular environment Produce enzymes that will make specific, controlled changes to an organism’s genome...contains multiple protocols and troubleshooting tips to make plasmid usage as simple and straightforward as possible...
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