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We narrowed to 784 results for: SCR;

Showing: 151 - 200 of 784 results

  1. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...antibiotic’, very often we immediately imagine a prescription for a bacterial infection. While this post will...that the term ‘antibiotic’ can also be used to describe anti-parasitic drugs and anti-fungal drugs, because...development of resistance include (Figure 1): Over-prescription of antibiotics (for example, giving antibiotics...not finishing the whole course of antibiotics prescribed to them This may seem counter-intuitive considering...The take-home? Always take your medication as prescribed. How do we know which bacteria are resistant ...infection is still effectively treated. We can prescribe antibiotics for the shortest time period possible...

  2. Hot Plasmids - August 2020

    Type
    Blog Post
    ...protease allows a transcription factor to enter the nucleus and modulate transcription of the mCitrine ...your studies.  Find the plasmids to set up your screening systems at Addgene! Schematic of the evolution...their BioRxiv post, the authors generated and described several improved genetically encoded fluorescent...Takayuki Iwaki, Kimiko Hara, and Kazuo Umemura have described a simple method which alleviates the inclusion...activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed ...

  3. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...technique that uses a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver...use of introns, which will be removed from the transcripts in the plant but not from Agrobacterium. Test...transient leaf transformations are a great help in screening candidate genes and speeding up research. Certainly...Budak. "Virus-induced gene silencing, a post transcriptional gene silencing method." International journal...

  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...create secondary structures in RNA transcripts Effects on transcription (remember, it’s not all about translation...locations or fail to make it through the entire transcript resulting in the production of nonfunctional ...every amino acid I’d like to encode,” but, as described above, not every region of a protein should necessarily...translation - e.g. codon choice may interrupt transcription factor binding sites) As you might imagine,...algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization tools. Keep in ...

  5. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...They rely on a viral protein called reverse transcriptase to convert their RNA genomes into DNA to be ... larger protein: PR (protease), RT (reverse transcriptase), and IN (integrase). These viral packaging ...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain... cycle initially proceeds as usual. Reverse transcription of the transgene occurs in the cytoplasm, then... McCray, 2010). However, IDLV genomes are transcriptionally active, leading to transient expression of...Lentiviral vectors tend to integrate into transcriptionally active regions of the genome (Schröder et ...

  6. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram, which graphically represents... of the cell’s illumination (see Figure 2). H describes the maximum signal strength, while W results from...photocurrent vs. time. The resulting histograms is described through maximum current (H), the time the cell...depends on cell type and laser voltage. The voltage describes an electric potential that can be applied to the...comparing two markers simultaneously, we commonly describe the resulting populations as double positive (...that there are three parameters — H, W, and A — describing the intensity and duration of the detected signal...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... rounds of screening. Created with Biorender.com.   Some phage display libraries screen antibody genes...expression systems. scFvs are also small enough to be screened for with in vitro display methods such as phage...and VH sequences. Phage display also allows for screening scFv generated from the B cell antibody genes ...the ways antibodies are. scFvs are even used to screen for new antibodies. To do this, antibody variable...variable domain sequences are expressed as scFvs and screened with in vitro assays to select for strong binders...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... are isolated from the resulting colonies, and screened for the desired modification. Finally, the positive...final circular plasmid product pictured here. B) Screening of recovered plasmids for the mutation of interest...mutation). Similar results can be obtained with PCR screening using the primers specified by the arrowheads ... vastly facilitates the subsequent process of screening for succesfully mutated clones. Forward and reverse...can also form colonies under these conditions. Screening If a restriction site was introduced (or ablated...ablated), bacterial colonies can be screened by identifying the presence (or absence) of that particular ...

  9. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids. Selected...2017). AAV-PHP.eB has frequently been used for screening candidate regulatory elements such as enhancers...type-specific promoters or Cre lines. Spatial transcriptomics suggests that AAV-PHP.eB, when paired with ...capsid of choice for future promoter/enhancer screening. However, AAV-PHP.AX packages viral genomes at...target gene expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested ...when using a ubiquitous CAG promoter. Spatial transcriptomics shows that its tropism is relatively more biased... Gradinaru lab. It was selected from a library screened in marmosets. AAV.CAP-Mac effectively transduces...

  10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...from the event can be found below along with a description of the program beneath it. You can jump to the...the different time stamps in the description to watch any sections you’re particularly interested in, ...abbreviated methods section found within a published manuscript. Luckily, many organizations have been working...JoVe, and Bio-protocol all work to provide more descriptive and interactive protocols that make it much easier...would get in-principle acceptance of the final manuscript regardless of whether the ultimate results were...Minisymposium on Reproducibility Booklet - Including descriptions of many organizations promoting reproducibility...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...and probe with live cells The following is a description of a technique published by Simpson, Sacher, ...be cloned into E. coli and subsequent proteins screened for cell-binding abilities (6). This can be accomplished...long-term library storage. After overnight growth, scrape up, pool and extract plasmid DNA directly from ...construction for functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 ...Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology...

  12. What's New in CRISPR - September 2019

    Type
    Blog Post
    ... fluorescent proteins, fusion proteins, or transcriptional regulators. In this system, Cas9 generates ... Find the CasX plasmids Drug inducible transcriptional activation and genome editing Yu Wang's lab...developed drug inducible CRISPR/Cas9 systems for transcriptional activation and genome editing by fusing Cas9...library with SpdCas9 to perform pooled CRISPRi screens to identify essential genes and phage host factors...metastasis cohorts and a prior mouse metastasis CRISPR screen. The Chen Mouse Tumor Suppressor Gene CRISPR Knockout...

  13. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench ...submission of a job application. 3) Read job descriptions carefully Be open minded and be bold. You don...qualifications to apply for a position.  A job description is usually a wish list and the hiring manager... way to a hiring manager. Learn to confidently describe your strengths in your resume and don't be shy...

  14. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...efficiency! Screening mutant libraries After generating your mutant libraries, you have to screen thousands...generating the library of mutants and how they are screened. When conducting antibody evolution experiments...across large mutant libraries, high-throughput screening techniques such as ELISA and cell surface display... on the surface of cells. This enables direct screening via flow cytometry for improved binding under ... for Protein Engineering: library generation, screening, and affinity maturation. Methods in Molecular...

  15. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...sequence prior to an ATG start codon to prevent transcription in cells where the recombinase was not present... transgene into the correct orientation for transcription. Upon recombination one of lox2272 sites will...expression. To test their hypothesis, they randomly scrambled one pair of lox sites and either used the same...transgene (homologous pairs) or used different scrambled sequences to flank the transgenes (non-homologous...amplification. On the left homologous pairs of scrambled cre-recombinase sites lead to greater frequency...leaky expression. On the right non-homologous scrambled recombination sights lead to reduced reversion...

  17. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...make the screen-sharing experience more interactive. The button is on the right of the Share Screen control...control panel on the top of the screen and every participant get to annotate things as they come up in their...their minds. The final screen can also be saved and exported as a picture. I believe that showing something...pivotal in writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens ...

  18. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...of Amsterdam. The previous post in this series described a practical approach to selecting a bright fluorescent...literature are performed in different ways. We describe a number of issues with some of the experimental...fluorescence, which, in its simplest form, can be described by a mono-exponential decay (analogous to radioactive...). Consequently, the intensity decay is often described by the t½, which is the time after which half ... al, 2008; Bindels et al, 2017) and cannot be described by a single parameter. Therefore, it is necessary...

  19. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...or gene copy. The coding sequence for GFP is transcribed and translated along with the gene, producing... last exon of the gene to ensure it would be transcribed and translated. For an N-terminally tagged protein...each cell line. The editing strategy and design described above can be used to create similar donor plasmids...Endogenous gene tagging with fluorescent proteins as described here enables live cell imaging to study cellular...

  20. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...CRISPR pooled libraries that allow functional screening of thousands of genes in genome-wide experiments...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd...regulation, gene of interest expression, knockout, and screening. There are many more useful viral vectors available... lab. Learn more about CRISPR plasmids for transcriptional inhibition. Retroviral Plasmid Kit Retroviral...collection can be used for in vivo phenotypic screening of small-molecule libraries and can be combined...

  21. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...template with the endogenous gene. Here, we'll describe the techniques and the plasmids used to inactivate... a rare event, so you must select as opposed to screen for cells where recombination has occurred. Neomycin...Conditional knockouts The methods and plasmids described in the preceding sections are simple ways of knocking...knockout, researchers often use the Cre/Lox system described earlier. In this case, you design your targeting... this gene will still be expressed. You'd first screen for this specific recombination outcome using PCR...

  22. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...user wants to carry out a high-throughput genetic screen, it is expensive and time consuming to individually...each insertion. Also, it remains challenging to screen for the genetically modified strains that contain...simultaneously introduce a selection marker for screening modified strains. With SapTrap, the user first...selectable marker (floxed Cbr-unc-119) for easy screening of the insertion event, and a variety of connector...hundreds of genes, generating powerful genetic screening libraries. Additional protein tagging systems ...

  23. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong et al. (2013) Science ... The human U6 promoter prefers a 'G' at the transcription start site to have high expression, so adding...co-transfect in HR donor. Alternatively, a new system described recently is the double nickase system, with PX335...destroy the BbsI sites, a double digest should discriminate between positive and negative clones. Clones..., how can cells with that mutation be selected/screened? A13: Before starting your experiment, consider...sequencing, for example) on each colony in order to screen for positive ones.  -- OR -- If your HR template...

  24. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...developed a separate script for manually inputting known innocuous differences. This script compares the sequence...disregards the specified discrepancies. Any sequences that are a perfect match from this script can then continue...time, but does not leave much flexibility for discrepancies in backbone sequences. Most theoretical sequences...

  25. Hot Plasmids - November 2023

    Type
    Blog Post
    ...in vitro and in vivo. Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp ...LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID, where two proximity labeling enzymes...recently created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion... HaloTag dyes and showed they could record transcriptional activation events as bands of induced GFP-iPAK4...Analyzing the ticker tape images could recover transcriptional histories, accurate to within 40 minutes. ...

  26. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...you can use to study these genes! Groucho, a transcriptional co-repressor The first conference room, that...Addgenies, was named after the protein Groucho, a transcriptional co-repressor important in development. Groucho.... It binds to a specific set of DNA-binding transcription factors to repress the expression of genes primarily...Ish-Horowicz. "The Groucho/TLE/Grg family of transcriptional co-repressors." Genome biology 9.1 (2008): ... Jiang, Jianqiao, and Helen White-Cooper. "Transcriptional activation in Drosophila spermatogenesis involves...

  27. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...If you’re new to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role...MECP2 in Rett Syndrome Rett Syndrome was first described in 1966 by Austrian physician, Andreas Rett, who...maturation (Tillotson & Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have...have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013). Mutations that affect ...

  28. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...specialized strains are available to confer greater transcriptional control, assist with proper protein folding...Lacks functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm...addition of IPTG. T7 RNAP is then available to transcribe the gene of interest from a T7 promoter on the... the expression plasmid in order to repress transcription from a hybrid promoter. Although the DE3/T7 ...don't see protein overexpression? The strains described above should generate sufficient expression levels...

  29. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...cause off-target effects in a variety of RNA transcripts independent of Cas9 targeting (Rees et al., 2019... be targeted (Walton et al., 2020). The initial screen for base editors that could target KJ’s mutation...PAMs available, a large number of gRNAs could be screened for the most efficient editing. In these initial...sequence near this site is AGC, so in a secondary screen, the team tested Cas9 mutations that imposed a ...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...

  30. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...Jennifer and I spent about an hour that morning scrambling. Everything we had set up involved multiple people...people in tight locations. Conference rooms for on-screen presenters! Labs and benches! Everything was out...videos. It’s really nice to have a scientist on-screen, somebody who really knows the protocol and can...together a video from their recorded answers. No scripting made this a lot easier and I didn’t need to be...where I could focus on camera and audio, and the on-screen talent could just focus on their interview or performance...

  31. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...genes from a single transcript, or if you want to prevent read-thru transcription from resulting in the...3509-3514. Tikhonova, Elena B., et al. "A transcription blocker isolated from a designed repeat protein...protein combinatorial library by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid ...the production of a downstream peptide, the transcription terminators in this series are just the tool...

  32. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...therapeutics - one using dCas9-SAM to activate HIV-1 transcription and destroy infected cells, the other using ...histone deacetylase (HDAC) inhibitors to increase transcription of the integrated HIV-1 genome. For a targeted...dCas9-VP64 fusion commonly used to activate transcription, but with two additional activation domains ...domains (MS2 and p65) to enhance transcriptional activation. Zhang et al. designed multiple gRNAs to target ...to Use CRISPR Pooled Libraries for Genome-Wide Screens Additional Resources onf Addgene.org Check ...

  33. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...R26R-Confetti described in Hugo J. Snippert, et al. (2010) and the MAGIC Marker strategy described in Karine...overcoming the limitations listed above. First, they screened a variety of fluorescent proteins to find those... via immunostaining. This feature facilitates screening of Cre-negative Brainbow mice to determine the...addition of a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to...

  34. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...article describing a sucrose supplementation strategy to improve yield. In addition, as described in this...you time and improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification ...lab tips, and more video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources...

  35. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...Find the anti-CRISPR plasmids Tagging human transcription factors with GFP Kevin White’s lab recently ...deposited a set of CRISPR plasmids to tag human transcription factors with GFP. As part of the Encyclopedia...these donor plasmids will help researchers map transcription factor localization and binding via ChiP-seq...ChiP-seq. Learn more about ENCODE Find the transcription factor tagging plasmids If you have a new CRISPR...

  36. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...CRISPR technology has brought drug and genetic screens together and expedited the drug discovery pipeline...Alternatively, a disease model can be generated, and drugs screened for a change in pathology. If the disease pathology...accelerate the drug pipeline. High-throughput CRISPR screens can be used to inhibit, activate, or knockout many...identified several candidate inhibitors of gene X in a screen. You generate a gene X knockout in a cancerous ...

  37. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...responsibilities of a role, so it helps to see how people describe their actual work. To do this, try looking at ...to present your work at conferences, help with manuscript and grant preparation, and lead your own research...

  38. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...tools in Drosophila (Fig. 1):  GAL4-UAS, a transcription factor binding site. GAL4 expressed in the fly...site to cleave and inactivate any read-through transcripts of the effector gene. Effector genes, any gene...across cell types and organisms by replacing the transcription factor binding site and effector in the construct... the SPARC system to use other experimental transcription activation systems, such as LexA/p65 or mCD8...

  39. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ...Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon on the right?!? ... course more quickly than writing scientific manuscripts and, as one of my supervisors warned me, the ...’t have to mean abandoning it or running away screaming, but instead can involve walking alongside it ...it and giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share ...

  40. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...fly fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome... Mehmet Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease...also got to show off our new CRISPR page (which describes how to plan your CRISPR experiment for our new...next week, so follow us on Facebook, Twitter, or subscribe to this blog. Find more CRISPR and Genome Engineering...

  41. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...see: Uhlen et al., 2016). In this post, we will describe the approaches that can be used to determine selectivity...of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...commercially available. Various proteomic and transcriptomic datasets can be used to select a candidate ...recognized by either antibody was not shared. Tables describe that the antibodies both recognized the target...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ...

  42. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...CRISPR pooled gRNA libraries that can be used to screen for genes involved in a wide variety of cellular... infect target cells, and, finally, conduct the screen. To help alleviate some of this labor for our ...optimization-scale infection and at least one screening-scale experiment achieving the gRNA representation...) says: "When you're setting up to do a genetic screen, you need to focus on building a really good model...

  43. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ... lacks context unless it links to a verified description of that reagent online. Furthermore, if the link... link between the catalog number and reagent description is lost (e.g. a supplier is bought out by another...specific information about the plasmid: name, description, how the plasmid was generated, validated sequence...our example plasmid, lenti-Cas9-VQR-Blast. If you scroll down to the bottom of this plasmid’s page you will...

  44. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    ...more detailed level, the Gal4/UAS system is a transcription activation system co-opted from yeast (1). It...workhorse to turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences...sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression. Thus...Lai, T. Lee, Genetic mosaic with dual binary transcriptional systems in Drosophila., Nat. Neurosci. 9, 703...

  45. Components of CRISPR/Cas9

    Type
    Blog Post
    ... of the major CRISPR lingo and will be able to describe the functions of the various CRISPR/Cas9 components...Bind to a guide RNA The guide RNA, which will be described in more detail below, enables Cas9 to cut a specific...arrays as found in the bacterial genomes are transcribed into pre-crRNAs containing both the spacer region...RNase III, the tracrRNA and Cas9, bind to these transcripts and C) cleave them leaving mature crRNAs bound...

  46. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...responses are varied; one outcome is directed transcription from a given promoter. To harness the power ...the RR (OmpR) via phosphorylation, promoting transcription (see figure below). Subsequent exposure to red...response regulator via phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red...types of proteins, including Cas9 and dCas9-transcription factors, for which low expression levels are...

  47. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...catalog – so check there before building one from scratch! Why deliver these systems virally instead of by... preserving cell viability. Library delivery Screening libraries, large collections of biological independent... entire genome, overexpression of all human transcription factors, barcoded libraries to trace cell lineage...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...

  48. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...plasmid to express the target antigen could be screened in a high-throughput manner. Cells were labeled...which was observed for the hybridoma mAb.  While screening by IF-ICC worked well for identification of the...was recognized that an aberrant IgG light chain transcript was expressed by some of the hybridomas and, ...limited the number of clones that needed to be screened through functional assays. Following functional...

  49. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et al., 2015...fusion to a receptor-transcription factor fusion. TEV protease cleave the transcription factor from the receptor...

  50. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain the expected...of plates will have select samples prepared and screened by NGS to spot check the samples, the rest will...are requested by scientists, will immediately be screened by our QC scientists to confirm the plasmids contain...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...
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