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We narrowed to 255 results for: tre promoter

Showing: 151 - 200 of 255 results

  1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...characterize the function of certain regions in a promoter or gene, as well as to study the effects of inactivating...methods, chances are you’d like to find a better one. Trehan et al. wondered if, instead of relying on in vitro... each other; ~17 bases is optimal. After being treated with DpnI to digest away the template plasmid, ...applies to deletions; using REPLACR-mutagenesis, Trehan et al. deleted a whopping 144 kb from a bacterial...recombination. Across all modifications described in Trehan et al., REPLACR-mutagenesis’ median efficiency ... for site directed mutagenesis   References 1. Trehan, Ashutosh, et al. (2016). “REPLACR-mutagenesis:...

  2. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...forms as part of graduation, hiring, funding, and promotion processes, I’m confident that everyone’s enthusiasm... review and primary research journals including Trends in Cell Biology, Cell, Molecular Cell, Developmental...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...fluorescent proteins and is driven by an inducible promoter (Plasmid 64542 or 64543) Before translation, ...cellular function, especially during development or stress. With ribosome profiling, researchers have been...

  4. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ... and funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity... order, or every combination of materials and treatments is all too often glossed over. This is why it...which data are investigated to look for unexpected trends or differences between groups. Hypothesis generation...hypothesize that growth rates will be higher in treatment versus control, and you think there are four important...

  5. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...and tech transfer communities who help guide and promote Addgene in its mission to make research easier.... interesting biological questions, you need a tremendous amount of technology development alongside it...what is worth having. The work that [Addgene’s] outreach scientists do to comb through the literature, ...

  6. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet aggregation, and ... that fasting has a positive effect on cancer treatment (Ref 1). As described in the presentation, starvation...favorable state for tumor progression (Ref 2). Future treatments may need to target this immunosuppressive reaction...

  7. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...visualization and expressed from a heart-specific promoter. Fish were labeled with MG-2I, either by incubating...hearts 24 hours post treatment and no mCer3 cells present 96 hours post treatment (see figure 2). Induced...culture experiments, only dL5**-expressing cells treated with MG-2I dye and exposed to light stained dead...Chromophore-assisted laser inactivation in cell biology. Trends in Cell Biology,18(9), 443-450. doi:10.1016/j.tcb...

  8. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...assembly reaction. DNA fragments with the desired promoter, selectable marker, and target gene, for example...and improvements in these methodologies offer streamlined and simplified alternatives to traditional cloning...

  9. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized...straight-forward. All constructs are tagged with a 2xStrep-tag for easy detection and affinity purification...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...studying how that one protein interacts with that one promoter can tell you, it can be easy to get lost in the...Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts in the Microbiology Community Sign Up ...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...changes were introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and...bacteria resistant to both spectinomycin and streptomycin, but could also be used for studying epistatic...

  12. Depositor Collections

    Type
    Collection
    ...Community (MCC) Collection Pleiades Promoter Project: mini-promoters to drive selective expression in the...Syndrome Plasmid Collection and Resource Center TREAT-AD Plasmid Collection Visualization Michael Davidson...

  13. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated regions — on gene ...Cofactors/substrates Required Not required Signal strength Lower Higher Sensitivity Higher Lower Background...gene expression. The regulatory element is cloned upstream of the luciferase gene, and expression of luciferase...

  14. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Viral expression cassettes typically contain a promoter (inducible or constitutive), your gene of interest...the last decade, viral applications in cancer treatment and gene therapy have also been in the limelight...approved the first oncolytic viral therapy in 2015 to treat melanoma, and many more are in the pipeline. Viruses...

  15. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox...nucleosomes, loops, chromosomal domains, or how stretches of DNA on different chromosomes interact with ...K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural...

  16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ... 2008). Obligate dimers (such as dTomato) that promote interactions between the FPs interfere with native...Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced microscopy, University of Amsterdam...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced microscopy (University of Amsterdam...

  17. All in a Twist: dsRNA

    Type
    Blog Post
    ...-loops described above play distinct roles in promoting cellular processes and functions, but they also...production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing radiation, can also...

  18. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...complex, to temporarily inhibit this process and promote copying of the edited strand to the unedited strand... co-express mismatch repair inhibitor MLH1dn Promote desired resolution of heteroduplex     ✓ ...non-homologous end joining. As a result, Cas9 treatment causes most products to be indels while the efficiency... In addition to the numerous innovations that streamline the process of prime editing, the Liu lab contributed...efficient as possible. Figure 2: Decision tree for selecting a PE6 variant. PE = prime editor; ... 2023) Prime editing has a lot of promise for treating genetic diseases. In our summer Hot Plasmids post...

  19. Plan Your Experiment

    Type
    Collection
    ...template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing efficiency...prime editing. CRISPR Intereference : Target the promoter region of the gene of interest, where the Cas9...approaches, the species and expression pattern of the promoter for both your Cas enzyme and gRNA and the presence...the wide range of options for the Cas enzyme, promoters, and selection markers. There are also no packaging...to specific cell types by using cell-specific promoters, which is useful for in vivo studies. While we...transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery . As mentioned...protein product. Be aware that there could be downstream translation start sites that could allow for ...

  20. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...several weeks which may also depend on transgene or promoter choice. Behaviors you can study using viral vectors...into the experimental setup, the mouse becomes stressed. It will likely move around faster in the following...some time to adapt to a testing apparatus. General stress levels of the test animal may also be reduced by...noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider:... groups), health status, diet, cage enrichment, stress level, origin (vendor or in-house mouse breeding...

  21. Worm Expression Resources

    Type
    Collection
    ...recombinase from either a heat-shock promoter or a tissue-specific promoter and expression of the target FLP-out... from either a ubiquitous or a tissue-specific promoter. cGAL and Split cGAL plasmids - Paul Sternberg...Jorgensen Lab. A collection of plasmids that streamlines the production of targeting vectors for tag insertion...

  22. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...nuclear localization, binding to the appropriate promoter sequence and activation of gene expression. In...bulk cell experiments, Clift et al. observed no stress or damage upon TRIM-Away, and the electroporated...re-adhered to the culture plate within 4 hr of treatment. In some primary cell lines, endogenous TRIM21...Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system...

  24. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...science and engineering. “Fix the Institutions” promotes gender equality in careers through structural ...factors may include cell type, the disease being treated, and other hormonal, immunological, and environmental...

  26. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...dopamine is an important neurotransmitter that promotes reward-motivated behavior, if the channelrhodopsins...as predict the channelrhodopsin’s photocurrent strength, wavelength sensitivity, and off-kinetics.   This...did not turn. Left-turning behavior in ChRger2-treated mice ceased when the light was turned off.  Together...

  27. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm ...of two sgRNAs from independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for...along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned into separate vectors and then... assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one...pGGDestTol2LC vector. Uses five distinct zebrafish U6 promoters for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs...expression of two gRNAs from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous ...Zhang Cas9/pTREX-n 68708 Other/Trypanosoma cruzi yes, cut S. pyogenes Neo Docampo tdTomato/pTREX-b 68709 ...

  28. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...gene expression and the selection of specific promoters may also help AAV vectors evade host immunity....potentially be treated by correcting the mutation or replacing the gene. A notable example is the treatment of Severe...conditions, opening up new avenues for therapeutics that treat disease at the molecular level. For instance, if...function. To date, gene therapy has been used to treat 10 infants with this disease. To do so, their T-...childhood onset of blindness and was thought to be untreatable until 2008. Following a single dose of subretinal...in vision over a period of 1 to 3 years – the treatment appears to be both safe and effective. Challenges...antibodies that neutralize the AAV vectors used to treat them. Scientists are working to overcome this issue...

  29. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...discussions. Get your voice out there. Use Twitter to promote your other science communication activities, e....engagement - Participate in science festivals and outreach If you are comfortable talking in public, volunteer...groups, charities and NGOs are often looking for outreach volunteers as well. 4) Blog - Hone your writing... a blog is not only a great form of scientific outreach, it also helps you to hone your writing skills...science communication. The National Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site ...

  30. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...incorporating it into a fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated...experience of end-users, size of the transgene, and downstream applications should all be considered when choosing...factors such as resources available, cost and downstream applications. For example, while the PEG precipitation...be the best method when pure virus is required downstream. When assessing lentiviral titer, don't compare...

  31. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...its paralog RC2H2 are RNA binding proteins that promote RNA decay and their upregulation is correlated ...little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared ...

  33. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Program at Harvard Law School. He has continually promoted the cause of increasing data collection about ...Symposium Boston 2015 you can register here or live stream the event on October 22nd and 23rd here. Many ...

  34. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...

  36. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... had multiple integration events. In addition, promoter choice can also impact transgene expression. The...transduced with serial dilutions of a viral prep, treated with the antibiotic, and colony-forming units quantified...

  37. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...surprise since the PAM flexibility could in theory promote off-target binding. The activity of SpRY was validated...precise targeting (give or take a bp or two upstream or downstream) for most gene targeting experiments. Between...

  38. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...decreases. Hypermethylation of CpG islands in the promoter region of tumor suppressor genes can repress their...study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving...

  39. PhD Applications After COVID

    Type
    Blog Post
    ...universities stressful or simply not feasible. Through the virtual systems created and promoted during COVID...fit for you without the financial and logistical stress that accompanies traveling for campus visits. This...have to worry about traveling during an already stressful time, and I was more relaxed during both sets ...their end. Though the quick changes in plans was stressful in and of itself, the flexibility allowed through...using the virtual world to your advantage can strengthen your application and help you decide if a program...

  40. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...complexed with a gRNA that directs them to the promoter of a gene of interest, they have been shown to...conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools...complex. When this complex is directed to a target upstream of the transcriptional start site (TSS) of a gene...competent to cut DNA. When directed to a site downstream of the transcriptional start site of a gene of...

  41. dTAG - You're it!

    Type
    Blog Post
    ... al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.... Test dTAG molecule toxicity in new cell lines Treat parental cells (or cells expressing a control --...

  42. Hot Plasmids - October 2020

    Type
    Blog Post
    ...express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library...Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-...

  44. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...gene expression in C.elegans. In this system a promoter controls the expression of a GAL-4 driver that...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

  46. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...walk into any supermarket now without seeing a promotion for antibiotic-free, farmed meats. We can focus...antibiotics. When we use antibiotics to treat infections, we are treating microbes with compounds similar to...will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection with...more different classes, or types, of drugs used to treat them. Very often, this is what it means to be a ...often very difficult and very time consuming to treat. Learn How Antibiotic Resistance Genes Are Used ...antibiotics in livestock farming Lengthy courses of treatment with antibiotics These scenarios expose bacterial...the population and making them more difficult to treat in the future. Patients not finishing the whole...

  47. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...activity Regulating Acr proteins by inducible promoters, light, or small molecules could achieve rapid...meningitidis (Nme) (Pawluk et al., 2016). While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and...system. 5. Phage therapy Phage therapy uses phage to treat bacterial infections as an alternative to antibiotics...

  48. Zhang Lab CRISPR Page

    Type
    Collection
    ...Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; for sgRNA...tyroxine binding globulin (TBG, PX602 [#61593] ) promoter, and a U6-driven single guide RNA. The vector ...codon-optimized SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in...FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  49. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...on the Addgene Blog Origin of Replication The Promoter Region – Let's Go! Browse All of Our Plasmids ...in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated cells—but...be determined empirically.  Negative controls: untreated cells, empty vector control, and non-targeting...is observed). In any plasmid-based experiment, untreated cells should be included as these provide the ...

  50. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure 1...dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of the self-labeling tags ...Turner, E.D., Abdelhamid, M.A.S., Craggs, T.D., & Twelvetrees A.E. (2024) Kinesin-1 is highly flexible and ...
Showing: 151 - 200 of 255 results