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We narrowed to 201 results for: ADAP

Showing: 61 - 80 of 201 results
  1. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...
  2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...applications. The first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1...targeting flexibility. Cas13a (formerly C2c2) has been adapted for targeted RNA cleavage in mammalian cells. The...
  3. Bioinformatics at Addgene

    Type
    Blog Post
    ...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...
  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...
  5. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY license. ...transduction efficiency for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license...
  6. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license...
  7. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...Mensah & Niskanen et al., 2023). Now, they have adapted the 17-residue peptide that caused nucleolar disruption...Constructs for tet-inducible CRISPRa-AAV engineering. Adapted from Nguyen et al. (2025) under CC BY license. ...
  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Rising τ, and Decay τ of the three sensors. Image adapted from Aggarwal et al. 2025 under a CC-BY-NC-ND 4.0...displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et al. 2024 bioRxiv...
  9. Finding Your Perfect Job After University

    Type
    Blog Post
    ... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ...
  10. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... you probably realize by now, MoClo is a very adaptable system with lots of tools and reagents already...acssynbio.3c00423. Pérez-González, Ana, et al. “Adaptation of the GoldenBraid Modular Cloning System and...
  11. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...black is the fusion tag or labeling system. Image adapted from Lavis, 2016, under CC-BY. Common self-labeling...comparison with standard confocal microscopy. Image adapted from (Bottanelli et al., 2016) under CC-BY license...
  12. Fluorescence Titering Assay

    Type
    Protocol
    ...protocol was developed for 293T cells but can be adapted to a variety of target cell lines and selection...
  13. General Transfection

    Type
    Protocol
    ...HEK293T cell line should work. This approach can be adapted for different cell lines and different transfection...
  14. Colony Formation Titering Assay

    Type
    Protocol
    ...protocol was developed for A549 cells but can be adapted to a variety of target cell lines and selection...
Showing: 61 - 80 of 201 results