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Addgene
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  3. Bioinformatics at Addgene

    Type
    Blog Post
    ...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...

  5. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY license. ...transduction efficiency for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license...

  9. Finding Your Perfect Job After University

    Type
    Blog Post
    ... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...black is the fusion tag or labeling system. Image adapted from Lavis, 2016, under CC-BY. Common self-...comparison with standard confocal microscopy. Image adapted from (Bottanelli et al., 2016) under CC-BY license...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... you probably realize by now, MoClo is a very adaptable system with lots of tools and reagents already...acssynbio.3c00423. Pérez-González, Ana, et al. “Adaptation of the GoldenBraid Modular Cloning System and...

  15. Fluorescence Titering Assay

    Type
    Protocol
    ...protocol was developed for 293T cells but can be adapted to a variety of target cell lines and selection...

  16. General Transfection

    Type
    Protocol
    ...HEK293T cell line should work. This approach can be adapted for different cell lines and different transfection...

  17. Colony Formation Titering Assay

    Type
    Protocol
    ...protocol was developed for A549 cells but can be adapted to a variety of target cell lines and selection...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...protocols, visit https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com...New World Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a...

  20. Western Blot

    Type
    Protocol
    ...protocol uses a dry transfer device but can be adapted for wet and semi-dry transfer approaches. Last ...
Showing: 61 - 80 of 196 results