Skip to main content
Addgene
Showing: 61 - 80 of 161 results
  1. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...and tween as extended treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize...first to freeze everything in place followed by alcohol permeabilization. Users should also keep their ...
  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring...which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET efficiencies ...
  3. AAV Viral Preps

    Type
    Collection
    ...Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls...
  4. Antibody Production

    Type
    Collection
    ...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...
  5. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...acceptor molecules and can therefore be used to calculate the distances between the donor and acceptor. ...
  6. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA NADPH oxidase, EF-hand calcium binding domain 5 TAL3496 & TAL3497 TGCCGCCAACTGGCTGAAacctccagatctggagCAGAACAAGCGCAAAA...
  7. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team efforts of the Yit Heng...
  8. Rett Syndrome

    Type
    Collection
    ...Metrics Toolbox - algorithms for data analysis to calculate heart rate variability (Link opens in a new window...
  9. Deck the Lab 2022!

    Type
    Blog Post
    ... have decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing...
  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ...excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases...
  11. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... global coffee supply… My back-of-the-napkin calculations put the daily consumption here at ~ 60,000 cups...
  12. Editor's Choice, September 2016

    Type
    Blog Post
    ... can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards...
  13. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...
Showing: 61 - 80 of 161 results