We narrowed to 217 results for: Apple fluorescent protein

...selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution...development and applications of fluorescent RNA aptamers in Addgene’s collection. Green fluorescent aptamers ...small molecule activates the fluorescence of the small molecule. Fluorescent RNA aptamers are used to track... of highly efficient RNA mimics of green fluorescent protein." Nature structural & molecular biology 21.8... Resources at Addgene.org Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids...using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable! ...What is an RNA aptamer? RNA is not inherently fluorescent and can be challenging to track intracellularly...

...Photoactivatable Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with... other kinases and proteins. Zhou et al suggest that more photoswitchable proteins could potentially be... reporter plasmids Find photoactivatable fluorescent proteins in empty backbones in the Michael Davidson...Photoswitchable Fluorescent Proteins ... fluorescent proteins...transduction pathways are a lot like cell phone networks. Protein kinases deliver messages to the next members of... the active site. Dronpa also produces green fluorescence when exposed to 500 nm light.  Under 400 nm ...

...most commonly proteins. You can then use fluorescent microscopy to look for those fluorescent molecules, ... using fluorescence (for some background on fluorescence, try the Introduction to Fluorescence Microscopy...Introduction to Fluorescence Microscopy. iBiology. https://www.ibiology.org/talks/fluorescence-microscopy/...antibodies that have been joined to fluorescent molecules are applied to a cell or tissue sample where they bind...research applications of antibodies in Introduction to Antibodies Learn more about fluorescence microscopy...allows you to ask questions like: “Where does my protein of interest live within a cell,” “Does this disease..., knowing that where you see fluorescence indicates your target’s location. Some other terms you might...

...Epigenetics with a FRET Based Biosensor Fluorescent Proteins 101: Fluorescent Biosensors ...generate a fluorescent output, which will turn on, change color, or change intensity of fluorescence in the...fncel.2019.00474 Resources on Addgene.org Fluorescent Proteins: Biosensors Viral Service: Biosensor AAV...V., Fluorescent Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers...readouts for levels of different chemicals, hormones, proteins, etc., that you are interested in. The most obvious...and the receptor, but readouts could involve fluorescence, pH change, heat, etc. The last component of...readout for whatever the bio-recognition event is (fluorescence, pH, etc.). The transducer will generate a numerical...

...when paired with appropriate fluorescent optics. Existing red fluorescent voltage indicators, however,...inhibitory rhodopsin (Ace2N) with the red fluorescent protein (mScarlet) with either a seven or eight amino...zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination. Each cell...of colors depending on the combination of fluorescent proteins expressed. CreLite-mediated recombination...editors that can be reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme....31937940. Detection of neural activity using a red fluorescent genetically encoded voltage indicator Article...Genetically encoded voltage indicators (GEVIs) are proteins that detect and output the voltage changes in ...

...expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression in ...EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and... : 285 vectors for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB ...

... 2015). To do so, they fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9,... or boxB) that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of...MS2-PP7) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used to visualize relative...blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR blog posts Resources...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without...to the small molecule, DFHBI-1T to induce its fluorescence. They found that aptamer insertion in the gRNA...8XMS2 which will recruit the HaloTag with red fluorescent ligands to the loci of interest. Dual color detection...

...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells... Biol 2018. PubMed PMID:29483642. Making protein-protein interaction studies easier with a 2in1 vector...The 2in1 cloning system is well suited for protein-protein interaction (PPI) studies in plants that rely...25971551. Discovery of new protein binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed...photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which change in...technique using small scaffold proteins to identify novel binding proteins that bind to either the light...cells because the protein binders can be customized for protein visualization, relocation, degradation,...

...years, we seek applications for awards to early career scientists using fluorescent proteins in their research... in the development and dissemination of fluorescent protein tools. This year, we’ve made some changes...inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to monitor their localization...are co-expressed with a photoconvertible fluorescent protein mEOS4a. Cells containing a sensor that shows...-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling...disease,” says Aggarwal. This biosensor changes fluorescence based on the release of glutamate from neurons...thiol-containing acceptors. These hydropersulfides modulate protein activity and have important roles during oxidative...

...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors...pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more. Addgene...Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral.... Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs...RESOLUTE Plasmid Collection for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...

...where an antibody binds a target protein in your sample, then a fluorescently labeled secondary antibody binds...suitable antibody against your target protein, consider fusing a protein tag to the target and using an epitope-based...reagents like fluorescently-labeled nanobodies or scFvs. Many, many options for fluorescently conjugated ...Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence Resources...then you use a microscope to see where the target protein is (localization) and how much there is (quantification...quantification). But what if you’ve got several interesting protein targets and you want to know if they co-localize...labeled and imaged at a time, inactivating the fluorescent secondary antibodies before labeling the next...

...rest of the protein in place including FokI endonuclease domain as well as fluorescent proteins used to sort...with the same genetic background and do an apples-to-apples comparison of all those mutations. If you ...mammalian or other cells. TALENs are engineered proteins that comprise a DNA binding domain and an endonuclease...technology allows you to create a pair of designer proteins that will introduce a double-strand break in the...transfection or electroporation. All you do to create a protein that binds those 15 base pairs is take four plasmids...different genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare...

...photostable yellow fluorescent protein mGold2 By Mike Lacy Get your sunglasses — these proteins are bright! ...this post: Bright and photostable yellow fluorescent protein mGold2 Viral delivery of TnpB for plant genome...! Most current yellow fluorescent proteins tend to photobleach rapidly, which hinders their use for long-term... recently developed a pair of new yellow fluorescent proteins: mGold2s and mGold2t (Lee & Lai, et al.,...mVenus or mCitrine, or if existing yellow fluorescent proteins just haven’t met your needs, consider trying...al. (2025). Bright and photostable yellow fluorescent proteins for extended imaging. Nature Communications...Arnold Lab, ATLAS is based on an antibody-like protein AMPA.FingR (AF) that binds to AMPA receptor subunit...

...version at Addgene! Fluorescent protein and control AAV These fluorescent protein expression vectors can...exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging...exhibits high contrast between peak fluorescence and resting fluorescence.  It is useful for activity imaging...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium sensors and ...the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR and its...the control vectors for experiments encoding a fluorescent-tagged molecular tool.   Find pAAV-FLEX-tdTomato...of densely labeled neurons because background fluorescence from inactive neurons is reduced. 105322 ...

...vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...tools The luciferase reporter gene The green fluorescent protein reporter gene Resources on Addgene.org ... reporters Limited Depth of Signal/Imaging: Fluorescent reporters are not as well suited for imaging ...context/spatial localization: In vivo imaging with fluorescent or bioluminescent reporters usually only detects...ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles. These ~200nm...200nm long vesicles have hollow interiors and a protein shell that excludes water but is gas permeable. These... experiment to demonstrate the most exciting application for ARG’s: in vivo imaging. Specifically, arg1...

...can visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution... a single protein that can recognize the target protein (Wang et al., 2013). These proteins are also plasmid-derived...to study protein-protein interactions. Flow cytometry Flow cytometry is used to detect proteins on the ...scientists to target specific proteins they’re interested in. Once a protein is targeted with an antibody...entirety of the target protein: instead, it recognizes a small portion of the protein, known as an epitope... the target protein because the antibodies will recognize multiple epitopes on the protein. However, each...with other, similar proteins due to recognizing a single epitope of the target protein. However, small mutations...

...Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization...Research Campus reminds us that, although fluorescent proteins are incredibly useful, chemical fluorophores...different pH’s and some that are fused to fluorescent proteins. She also showed that R-bodies can rupture...Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to quickly...University, describes R-bodies, “force generating protein machines that can extend and retract over many ...Casilio, a system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety...genes in a single cell, and localization of a fluorescent signal to your target gene. Read this post to...

...for the amount of protein you are expecting.  Fluorescent proteins Fluorescent proteins, while more expensive...binding to your protein of interest. Protein preparation Before you can blot your proteins, you’ll need ...normalize your protein of interest to overall protein expression for each sample and protein. While ubiquitously...normalize your protein of interest to total protein expression. Total protein expression can be measured using...rely on primary antibodies conjugated to fluorescent proteins, instead of the more common chemiluminescent...consider the subcellular location of your protein. Proteins located in membrane-bound subcompartments,...considered if your protein is difficult to solubilize, such as a membrane-bound protein. There are many ...

...genetically encoded peptide (DogTag) and protein (DogCatcher); ideal for protein loop regions CRISPaint Gene Tagging...Tetracycline (Tet) transactivator to increase fluorescent protein expression C234-E25: attB2r-SNAP-attB3 ...HUH-tag For creating DNA-protein conjugates rHUH HUH-tag (RNA) Covalent protein tag for RNA labeling ...fused to other effectors from nanobodies to fluorescent proteins. So, I hope you will take full advantage...University of Cambridge. His research focuses on protein innovations for therapeutic and vaccine design....be similarly useful across the diverse kinds of proteins that one finds in cells. High yield of reaction...development of genetically engineered components, like protein tags (Figure 2). Through the work of many groups...

...Indirect labeling is achieved through protein-tag or protein-protein interactions, like biotin-streptavidin...transmembrane proteins, such as receptors, or post-translational modifications on those proteins, like glycosylation...kits allow for labeling of cytosolic proteins, as well as proteins of the secretory pathway. Those can ...buffer to label intranuclear proteins of interest, you can label cytosolic proteins alongside the intranuclear...that bind DNA or free amines of proteins. Depending on the application, they can be membrane-permeant ...like many of the cluster of differentiation (CD) proteins found on immune cells. However, there are more...methods can include labeling markers with multiple proteins/antibodies, targeting markers found within the...