...Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence wavelengths...importantly, these compounds cross cell membranes, thus enabling FRET measurements in intact cells. The Gradia ...However, proper control experiments are needed to establish the dynamic range of your assay. These experiments...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...similarly but with differing R0 values, thereby enabling calibrated FRET distance measurements (for an ...

...culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant pathways (Liu et al., ... Facultative anaerobe Facultative anaerobe Obligate aerobe Number of chromosomes 16 3 4 ... is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic yeast, meaning...

... a few model organisms, Micropublication will accept negative data or publish brief, novel findings. Most...for all ages. Organize your data and prepare publications or your dissertation Organize data that you... Most Micropublications contain one figure. If you aren’t there yet, write introductions to potential ...potential publications or prepare a review article. These are both options that can be done without having access...

...communication falls on the shoulders of journals and publishers. As journal editors, we often find ourselves ...contributions, etc.) before their paper can be published. To a scientist, all of this checking and formatting...can feel like yet another hurdle in front of publication when all they really want to do is get the paper...clear information about reagents, and why other publishers and organizations have various different versions...reproducible Read all of our blog posts on scientific publishing  ...

... pages in a Curricula Vitae to showcase your publications, academic performances, and research experiences...information. Think "skills," not just "research and publications” What are the skills you developed through your... grant application? Are you familiar with the publishing or patent application processes? How about facilitating... science? Or perhaps you have presented in some public lectures? These transferable skills could be meaningful...make their job postings and salary expectations public, so start by looking at these university postings...

...showing the workflow for the BLITZ system. Image from Xiong et al. 2021.    BLITZ uses the biotin ligase...makes use of pre-existing transgenic lines, termed BLITZ (Biotin Labeling In Transgenic Zebrafish) (Xiong...can be used for other experiments. And, while the BLITZ system was designed and optimized with zebrafish...can easily be applied to other systems.    Find BLITZ Plasmids here! Xiong, et al. eLife. 2021. https:...

...non-biologists alike. Given the public significance of this research, we have the obligation to discuss it as openly...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk...Since the embryos produced were not being used to establish a pregnancy, the researchers were able to examine...

...correctly to gap junctions (Shaner et al, 2008). Obligate dimers (such as dTomato) that promote interactions...CytERM with mTurquoise2 and dTomato, which are established monomeric and dimerizing FPs respectively, are...compared with mEGFP or mTurquoise2, which are established true monomeric fluorescent proteins. Concluding...aware that the fluorescent proteins reported in publications are only characterized under a limited number... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~... PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor...PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed...PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA... PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional Resources on the Addgene...

...outstanding achievement Evidence of published material in professional publications written by others about the... to obtain permanent residence. The recently established case Matter of Dhanasar provides the three-prong...requirements stress “educational background, expertise, publications, and plan for future activities; including the...to join (selected due to expertise in field). Publicity in major media and/or trade journals: You the ...therapeutics to cure the cancer. Authorship:  Publishing books, journals online and/or in print. Having...

... can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected to publish, but this can vary...require teaching experience or high numbers of publications, which may take a longer time to acquire. Learning...vary depending on which company you choose. If publishing is important to you, make sure that your project... proprietary compounds or information. Where publication is expected, the process is similar to academia...

... LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...activity.  The field of optogenetics is renowned for enabling precise temporal and spatial control. Optogenetics...dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control... and repeatable. Since these initial studies published in 2015, more split systems have been developed...Comparison of photoactivatable CRISPR strategies Publication System nickname Photoactivatable moiety Cas9 ...

...people or discussing science, it is a great way to establish yourself as a thought leader and have real conversations...Anything” was appealing, but also scary. I do a lot of public speaking where I enjoy communicating face to face...moderators for keeping the quality high and thus enabling a productive discourse. They even advised me on...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect...Channel Check Out Vidoes of Gender & Science from LabLinks  ...

...genes across the genome. Although this tool was published in 2011, it was only recently made available through...GATEway Cloning Frustrated by the complexity of assembling recombination of transgenesis constructs? A new...shRNA. This system requires less work than using established methods and minimizes some of the technical issues...the CRUSH vector in transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which...Clonetegration Classic genetic engineering methods enabling chromosomal integration of sequences in bacteria... gene primers from Table 2 of the associated publication which contain regions of sequence homology between...cloning strategy described in the associated publication can be used to generate any combination of tags...

...Wu Lab library can be found in the associated publication. Ma, et al. Cell Rep. 2015. PubMed PMID: 26190106...and ubiquitous reporter assay. In their recent publication, Kroeze et al. describe their enhanced TANGO ...constructed. The authors then used GMAP assembly to establish a collection of over 30 promoters and 140 genes...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website... order to improve the ease and convenience of assembling these multi-protein fusions or “polyproteams”...and MoClo. The EasyClone 2.0 kit has been made publically available through Addgene. Stovicek, et al. ...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository...

...reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized microtubule... binding of the plasma membrane. In previous publications, they described tools to control formation of...understand their roles in forming the cilia that help establish left-right organization of the embryo (Aljiboury...the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically controlled phospholipase...GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier...

...ethanol accumulates, it binds to the repressor, enabling it to bind the promoter and repress pADH1 activity... PMCID: PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic... PMCID: PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, Christopher J. Potter... PMCID: PMC3846601. Find plasmids from this publication at Addgene. Riabinina, et al. “Improved and ... PMCID: PMC4344399. Find plasmids from this publication at Addgene. Riabinina O, Potter CJ. The Q-System...

...Prepare by going sleuthing on the lab website, publication records, LinkedIn, ResearchGate or Twitter. Be...too, they may not have had experience writing, publishing, or discussing career opportunities with the ...the appropriate first authorship position on a publication of her project and made another lab member first...professional status – including belittling opinions, public professional humiliation, accusations regarding...connections such as serving on Boards? Does the publication record show evidence of industry collaboration...

...who creates human-animal hybrids. This book is published 30 years after the seminal work of Gregor Mendel...into the twenty amino acids. Once scientists established that DNA encoded all the information necessary...giants such as Isaac Asimov, Arthur C. Clarke, James Blish, and John W. Campbell. This period is termed as ...specific.  Jurassic Park by Michael Crichton was published in 1990, just a year after the discovery of meganucleases...Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher Correction: Programmable base editing of A•T to...

...research infrastructure that has taken decades to establish.These funding decisions will have far-reaching consequences... that scientific progress made through years of public investment isn't lost. Universities and research...biological materials during funding transitions. Enabling resource sharing: Our operating model allows researchers... expanding our collaborations with industry and public and private partners to create additional pathways...