We narrowed to 94 results for: CAG

1. Addgene's Website Update

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   Blog Post

   ...informed by Web Content Accessibility Guidelines (WCAG).  But don’t take our word for it — check out all...

2. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

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   Blog Post

   ...Marathon for the MGH Pediatric Oncology and the Chicago Marathon to benefit Neurofibromatosis, New England...

3. Reflections and Looking Ahead

   Type

   Blog Post

   ...with current Web Content Accessibility Guidelines (WCAG). These changes mean our content is both available...

4. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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   Blog Post

   ...Carolina at Chapel Hill University of Illinois Chicago                     Boston Children's Hospital                           ...

5. Identifying Sequence Elements with SnapGene's Feature Database

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   Blog Post

   ...Genetics and Cell Biology at the University of Chicago. He was an early depositor at Addgene, and conceived...

6. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

   Type

   Blog Post

   ... when they poked their nose into a hole in their cage. Since dopamine is an important neurotransmitter...

7. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   ...counterpart to this system utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon ...

8. Science communication: 9 Strategies to Get Your Foot in the Door

   Type

   Blog Post

   ...You can draw live sketches at conferences like @ATJCagan or create agar art with colorful microbes? Try...

9. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   Blog Post

   ...research: recommendations from the Future of Research Chicago Symposium F1000Research, 2016. Kyle T. Dolan, Joseph...

10. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ....org/10.1074/jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction...

11. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post

    ... D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome Replacement by Compound Autosomes...

12. DNA Purification Without a Kit

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    Blog Post

    ...method from the Bishop Lab at the University of Chicago doesn’t use actual milk to purify DNA, but rather...

13. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

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    Protocol

    ...AATTC and G - 3' to the top oligo and 3' - G and CAGCT - 5' to the bottom oligo, making our final oligos...Bottom oligo: 3' - G GTATAC AATTAATT CCGCGCGG GTTAAC CAGCT - 5' Note: We could leave off the 3’ G on each oligo...

14. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post

    ...commonly used model organisms like Arabidopsis and Medicago truncatula and several crop species including ...

15. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ... shown that the FANTOM database, which relies on CAGE-seq to directly capture the mRNA cap, provides the...

16. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

    ...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR...

17. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ... damage results in the transdifferentiation of glucagon-producing α-cells into insulin-producing β-like-cells...

18. AAV Titration by qPCR Using SYBR Green Technology

    Type

    Protocol

    ...'-GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve ...

19. AAV ddPCR Titration

    Type

    Protocol

    ...probe targeting ITR: ITR Forward Primer: 5’-CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT...

20. 28 Hot Plasmid Technologies from 2015

    Type

    Blog Post

    ...phototropin 1 (AsLOV2). In the dark, this peptide is caged by the asLOV2 domain and has reduced affinity for...