We narrowed to 95 results for: CAG

1. 15 Hot Plasmids from 2017

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   Blog Post

   ...enzymatic activity. In addition, proteins can be “caged” by fusion to specific domains thereby rendering... The authors use PhoCl in combination with this caging technique to beautifully demonstrate light-dependent...

2. #Deckthelab Contest Winners 2017

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   ...are some of our 2017 contest winners:   Chicagoland's Community Biology Lab (@ChiTownBio) and one ...

3. Addgene's Website Update

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   ...informed by Web Content Accessibility Guidelines (WCAG).  But don’t take our word for it — check out all...

4. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

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   ...Marathon for the MGH Pediatric Oncology and the Chicago Marathon to benefit Neurofibromatosis, New England...

5. Reflections and Looking Ahead

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   ...with current Web Content Accessibility Guidelines (WCAG). These changes mean our content is both available...

6. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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   ...Carolina at Chapel Hill University of Illinois Chicago                     Boston Children's Hospital                           ...

7. Identifying Sequence Elements with SnapGene's Feature Database

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   ...Genetics and Cell Biology at the University of Chicago. He was an early depositor at Addgene, and conceived...

8. Giving gRNAs a Facelift - Synthetic and Beyond

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   ...counterpart to this system utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon ...

9. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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   ... when they poked their nose into a hole in their cage. Since dopamine is an important neurotransmitter...

10. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...You can draw live sketches at conferences like @ATJCagan or create agar art with colorful microbes? Try...

11. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    ...research: recommendations from the Future of Research Chicago Symposium F1000Research, 2016. Kyle T. Dolan, Joseph...

12. Hot Plasmids and Viral Preps - May 2021

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    ....org/10.1074/jbc.RA120.01289     Novel protein cage provides simple enzyme protection in yeast bioproduction...

13. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ... D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome Replacement by Compound Autosomes...

14. DNA Purification Without a Kit

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    ...method from the Bishop Lab at the University of Chicago doesn’t use actual milk to purify DNA, but rather...

15. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

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    ...AATTC and G - 3' to the top oligo and 3' - G and CAGCT - 5' to the bottom oligo, making our final oligos...Bottom oligo: 3' - G GTATAC AATTAATT CCGCGCGG GTTAAC CAGCT - 5' Note: We could leave off the 3’ G on each oligo...

16. The Advances Behind The World's First Personalized CRISPR Treatment

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    ..., A. N., Robinson-Garvin, N. A., Small, J. C., McCague, S., Burke, S. M., Wright, C. M., Bick, S., Indurthi...

17. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...commonly used model organisms like Arabidopsis and Medicago truncatula and several crop species including ...

18. How to Design Your gRNA for CRISPR Genome Editing

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    ... shown that the FANTOM database, which relies on CAGE-seq to directly capture the mRNA cap, provides the...

19. Plasmid Cloning by PCR (with Protocols)

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    Protocol

    ...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR...

20. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ... damage results in the transdifferentiation of glucagon-producing α-cells into insulin-producing β-like-cells...