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We narrowed to 363 results for: Cre expression vector

Showing: 61 - 80 of 363 results

  1. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...restricts expression to GABAergic interneurons. These vectors can also be used as the control vectors for experiments...and control AAV These fluorescent protein expression vectors can be used to visualize cells and to quantify...pAAV-hSyn-mCherry at Addgene. Viral vectors coming soon! These vectors listed below should be released at...DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released so far... constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-...

  2. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post
    ... an episomal vector system that increases reprogramming efficiency through the expression of a shRNA against...cells, thus expressing additional EBNA-1 transiently. The transiently expressed EBNA-1 increases protein ...RNA virus Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming factors...Reprogramming efficiency of PBMCs with episomal vectors Episomal vectors are based on the Epstein-Barr Nuclear ...long-term expression of the reprogramming factors. However, reprogramming of PBMCs by episomal vectors has ...protein expression from the other episomal plasmids. When used on PBMCs, the pCXLE episomal vector system increases...method for creating clinical-grade, safe, nonviral and nonintegrating hiPSCs where the vectors encoding ...

  3. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post
    ...promoter to drive expression of Cre-mCherry and a second promoter to drive expression of a Cre-dependent fluorescent...reporter. Expression of GFP will only result in cells where Cre-mCherry is also expressed. If Cre-mCherry...Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP expresses in horizontal cells...to test for cell type-specific expression of GFP. Half (113) expressed in retinal cells four weeks post-injection...More than 90% of expression occurred in one type of cell More than 50% of expression occurred in one type...of targeted expression. Surprisingly, <1% of synthetic promoters replicated the expression specificity...restrict expression to a single cell type based on the overlap in cell types both promoters expressed in. ...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post
    ...variety of pExpression vectors) plasmids. The type IIS restriction sites used in these vectors are of low...Confronted with the problem of creating vectors for predictable protein expression in bacteria, researchers ...SITS to create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins...or a mammalian expression vector in a one-pot reaction. Using this system, you can create custom RNA binding...easier to produce the constructs you need. Expression vectors within this kit contain a variety of promoters... oocytes. The expression vectors additionally contain various combinations of protein tags (His, Avi, ...Process with pCri Cell-free protein expression kit Cell-free expression systems have been improved for both...

  5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post
    ... tool for gene expression in mammals. Resources on Addgene.org Search for AAV vectors to use with your...pathogens to the CNS, but this also stops viral vectors, such as adeno-associated virus (AAV), from reaching...injection into the bloodstream, but requires a high vector dose and is unable to reach all regions of the ...phenotypic effects such as those that affected gene expression, mRNA splicing, or protein coding regions. In...one, Ly6a, showed differential protein and RNA expression between the brains of C57BL/6J mice and BALB/...strong linkage to the PHP.B phenotype and its expression pattern in the mouse brain.  Both labs then took...Deverman lab started by seeing how changes in LY6A’s expression affected PHP.B’s transduction rates. Knock out...

  6. Plasmids 101: Protein Expression

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    ...Gateway pDEST, and pBAD vectors for example. Protein expression from each of these vectors is controlled by ...resulting in different levels of expression from each vector; lower expression may be required if your protein... and cell free expression systems. Overall the general strategy for protein expression consists of transfecting...which expression system to use depends on several factors: The protein you are trying to express How ...system. Mammalian expression systems Mammalian cells are an ideal system for the expression of mammalian proteins...for mammalian expression utilize viral promoters (SV40, CMV, and RSV) for robust expression post-transfection...insect expression systems; baculovirus infected and non-lytic insect cells. Baculovirus expression systems...

  7. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    ... expression. CAG-FLEX: CAG promoter, FLEXed for expression in Cre-expressing cells hSynap: Human Synapsin...neuronal expression. hSynap-FLEX: Synapsin-1 promoter, FLEXed for expression in Cre-expressing cells. hSynap-FLP...systems you can use to express the iGluSnFR3 variants: pRSET: Bacterial expression vector, use with IPTG induction...transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol...FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for Glial expression (not neurons... eGFP for sfGFP to create SF-iGluSnFR. This improved variant has higher expression levels in bacteria ...range, photostability, quantum yield, and expression. Create yellow (v82) and lime green (v857) variants...

  8. Rabies and Neuronal Tracing

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    Blog Post
    ...Control Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression AAV for ...example, a researcher can deliver Cre-dependent TVA to Cre-expressing neurons in a transgenic mouse line...RABV expressing the viral protein EnvA on its surface will only infect Cre-positive, TVA-expressing cells...Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - ...to visualize how neurons are connected. Viral Vectors as Neuronal Tracers Due to their ability to efficiently...achieved by using EnvA-pseudotyped RABV, and only expressing the EnvA receptor (TVA) in user-defined cells... genome, so viral progeny will not necessarily express EnvA unless it is supplied in trans. Monosynaptic...

  9. Easing the Protein Purification Process with pCri

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    Blog Post
    ... information, you can then pick an expression vector and expression system. If you find that your protein... al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS ...protein might not fold well, you should screen a few different vectors to see which gives you the most functional... the secretory pathway to improve folding and disulfide bond formation. If one prefers expression in E...special E. coli strains designed for protein expression. Cultures are then lysed and the protein of interest...stability and solubility. Determining the best way to express one’s protein of interest can save a lot of time...pCri also includes plasmids designed for protein expression in systems other than E. coli, specifically the...

  10. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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    ...and efficiency for all-in-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs). Sakuma said...cloning method to ligate expression cassettes of the gRNAs into a single vector in tandem. They went on...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering...systematically established way of making an all-in-one vector for multiplex genome engineering.” Now, they’...Schematic overview of the all-in-one CRISPR/Cas9 vector construction system for multiplex genome engineering...their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully to target multiple sites in cultured...it has not yet been demonstrated, they say the vectors are also likely to work in animal embryos based...

  11. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ...Learn about Using Cre in Drosophila Read Other Genome Engineering Posts Use FLEx Vectors for Site-Specific... plasmids for overexpression and knockdown. Addgene also has a number of insect vectors to consider. An... your gene of interest in a plasmid and get the vector incorporated into the germline. To begin this process... browse Addgene’s Collection under the insect expression system or by filtering by fly as species of gene...Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly There are a few important...important points to consider when choosing the vector you’d like to use to generate your new fly line:... gene to be incorporated into the genome? Some vectors incorporate into the genome randomly, while others...

  12. Viral Vectors 101: Pseudotyping

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    ...lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using viral...Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods ... Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...virus. How to pseudotype To pseudotype your viral vector, you use the same protocol for generating lentivirus...Sometimes this is due to the cells that the viral vectors were produced in. For example, production of vesicular...virus, HIV-2, and human foamy virus in cell lines expressing galactosyl(alpha1-3)galactosyl (alphaGal) sugars...

  13. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...terminals with one vector. AAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane tethered...element containing vectors developed in the Jordane Dimidschsteind lab provide expression selectively in ...interneurons. We now provide ready-to-use AAV vectors expressing GCaMP6f with this promoter. Image calcium...dendritic neurites from axonal neurites with AAV vectors expressing cytoplasmic targeted tdTomato and pre-synaptic...this method, the major capsid protein VP1 is co-expressed with a fusion protein containing VP2C and a protein...These plasmids with different promoters driving expression of Cas9 were used in this study.  The zCas9i ... a plant cloning kit that contains a series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs...

  14. AAVs in Retinal Gene Therapy

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    Blog Post
    .... At the time, adenoviral vectors were the most commonly used viral vectors for gene delivery, and they...transiently. However new viral vectors capable of providing long-term gene expression in retinal cells - AAV ... better vector for ocular gene therapy: Adeno-Associated Virus (AAV) While other viral vectors such as...immunogenicity and its transient expression. LV results in long term expression in dividing and non-dividing...technician in a lab using adeno-associated viral vectors (AAVs) to treat an inherited retinal degenerative...tolerate the presence of antigens (such as viral vectors) without triggering a destructive inflammatory ...transduce retinal cells, AAV quickly became the vector of choice for ocular gene therapy. AdV and LV ...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
    ...transgene expression. Once packaged in AAV, this pre-recombined DNA could lead to transgene expression in recombinase-negative... their AAV vectors to find the optimal dose that allows for sufficient transgene expression but limits...blog Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We...producing multiple vectors in parallel because the risk of accidental errors increases. In addition to human... of poor reagent quality. To make sure our AAV vectors are of the highest quality, we undertake a rigorous...To make sure that adeno-associated viral (AAV) vectors available at Addgene are suitable for in vivo studies...serotype. Additionally, we check viral transgene expression in transduced cells whenever possible. This post...

  16. 28 Hot Plasmid Technologies from 2015

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    ...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT...luciferase) to create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed... to create the pCell-Free vectors, Gateway-compatible backbones which enable cell-free expression of proteins...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...-miR30 (CMV-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9...reaction with a desired destination vector expressing a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin...

  17. 27 Hot Plasmids from 2016

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    Blog Post
    ...AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ...easily create protein expression constructs fused with epitopes or fluorophores, generate expression vectors...large-scale expression screen that can be used to generate His-tagged human kinase constructs that express well... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic...

  18. Your Top Requested Plasmid in 2016!

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    ...This is a versatile mammalian expression vector that you can use to express both SpCas9 and your gRNA in...this is a versatile mammalian expression vector that you can use to express both SpCas9 and your gRNA of...the top requested plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang...mammalian cell types. Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter...resistance gene as separate peptides. PX459 This is the vector backbone. This particular backbone can be used ...N-terminus. This tag can be used to test for SpCas9 expression post transfection by western blot. Overall, this...mammalian cell lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9...

  19. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ... non-recombinant vectors (re-ligated empty vectors, for instance) would still express ccdB and therefore... years ago and developed cloning vectors to harness it. These vectors, called pKIL18 and pKIL19, contained...coli that were transformed with the empty vectors expressed the ccdB gene and were therefore unable to...insert into the vector, the ccdB reading frame would be disrupted allowing cells expressing the recombinant...cells will not propagate while expressing the gene. Briefly, the vector “backbone” in this system contains...cells expressing it? The answer lies in special strains of E. coli that tolerate the expression of the...ccdB makes cloning easier by selecting against vectors that did not take up your insert. But exactly how...

  20. Plasmids 101: Yeast Vectors

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    ...elements for vector propagation. This post, along with a future companion post on mammalian vectors, will catch...typically found as a single copy. Unlike the ARS vectors, CEN vectors are stable without integration. Yeast Episomal...of marker gene expression is to use a partially defective promoter to drive expression of the selection...to be created in advance of the experiment. The marker provided by the plasmid may be expressed at higher...plasmid maintenance within an E. coli cell, but vectors can be widely utilized across many different cell...core replication and resistance features of yeast vectors and explain how they differ from the bacterial ...previously described. Why do scientists use yeast vectors? Yeast are eukaryotes and thus contain complex ...
Showing: 61 - 80 of 363 results