We narrowed to 214 results for: DAP

...their devices, which makes it very difficult to adapt the device to one's own samples and limits the use...to as 'frugal science', existing components are adapted and combined with creativity and feedback from ...

...features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering endeavors. ...   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas proteins...

...packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to instead drive...delivering gene editing tools into cells. Image adapted from Segel et al. 2021.   Prime editing improvements...

...for assembling the barcoded gRNA library pool. (Adapted from Wong et al., 2016). With a list of gRNA...assembling barcoded combinatorial gRNA libraries. (Adapted from Wong et al., 2016). With CombiGEM and ...

...in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments in specific...transcription activation domains. Both systems are easily adaptable to neuroscience applications, including optogenetics...

...few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting the appropriate...single gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden...

...polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative ...Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012;337(6096):816...

... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016. Until recently...

...colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth.... PubMed Central PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent...

...section below.) For guide design, we decided to adapt the popular guide design tool Crispor by Max Haeussler...Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca Pinello and Kendell Clement for...

...MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools, they’re optimistic...optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing science...

...spreading within and between genomes, and have been adapted for a wide variety of applications - as mutagenesis...at two different incubation temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined...

...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...

...applications. The first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1...targeting flexibility. Cas13a (formerly C2c2) has been adapted for targeted RNA cleavage in mammalian cells. The...

...reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of concept...Gaudelli et al., 2017). GO is thus a flexible and adaptable tool to identify and enrich for base-editing events...

...studied in 40 labs as a great model for plant adaptation and speciation. Their phenotypes are diverse ...captivity. Sticklebacks demonstrate unusual parallel adaptation to disparate environments so can be used study...

...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...

...efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY license. ...transduction efficiency for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license...

...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...

...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license...