We narrowed to 145 results for: ENDOG
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TypeBlog Post...that do not express your protein of interest endogenously and transfect them with a plasmid encoding your...ultimately planning to use your antibody to detect endogenous levels of the target in other cells or tissues...
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Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...Additional Resources on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein...Protein Tags Fluorescent tagging of endogenous genes with SapTrap Resources on Addgene.org Browse CRISPR... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...a molecular species with high affinity for an endogenous molecular target, meaning the dye will be attached...results in direct binding between the dye and the endogenous target, and thus no genetically encoded tag is... -
Zhang Lab CRISPR Page
TypeCollection...complex for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...system described above, except that they use an endogenous nuclease system to cleave the gRNAs. gRNAs are...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” Proceedings of the National... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system Plant Utilizes the tRNA... promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple ... -
The 12 Days of CRISPR: 2021
TypeBlog Post... day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On... -
iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...plasmids can be used to create cell lines with endogenously-tagged gene variants or to differentiate human... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection..., and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class ... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post...Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled Unique advantages Mapping... -
Sensing Neuronal Dopamine
TypeBlog Post...to gather the following types of data: Report endogenous DA release from a single dopaminergic fiber in... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...organism’s genome (see figure) and to disrupt endogenous genes at the site of insertion. In both of these... -
Antibody Production
TypeCollection... been retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody)... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...Finger Arrays Engineered Zinc Finger Arrays for Endogenous Zebrafish Gene Targets Recent published reports... -
Antibodies 101: Epitope Tags
TypeBlog Post...separation of detection of a mutant protein vs. endogenous WT levels of the same protein when both are expressed... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...been to either systematically delete/overexpress endogenous genes or to introduce heterologous genes, but... -
DAP arrays for multiplex gene editing
TypeBlog Post...expression, the individual gRNAs are released by endogenous tRNA processing machineries, specifically, RNaseZ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...allows for whole organism imaging. They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...useful for therapeutic applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic...