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We narrowed to 270 results for: FAS

Showing: 61 - 80 of 270 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    ...perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline of individual steps ...could iterate our development and testing cycle faster due to the ease of jumping back and forth between... For example, our bbduk process created trimmed FASTQ files as output, which we specified as input to ...could adjust the compute resources when needed, for faster runtime and decreased cost compared to purchasing...

  2. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field, requiring people who are willing to... tasks, and then executing on them in a timely fashion while allowing for creative solutions to emerge...some of the more challenging parts of the job? With fast turnover of projects, pressure to maintain consistently...case, consulting can sometimes be challenging. The fast paced nature of projects often means working long...

  3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see Cre-lox ...Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how genes and proteins coordinate...9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces Gamma Rhythm and Controls ...

  4. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...coli multiplexing plasmid is available from the Koffas lab. Scaling up your multiplexed gRNAs with Golden... on a separate plasmid. Multiplexing in E. coli Koffas Lab CRISPathBrick Multiplex Plasmid: This system... E. coli CRMAGE Plasmids: The CRMAGE system is a fast, multiplexable method that combines CRISPR and recombineering-based...possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...with a confocal microscope, yet I was once again fascinated by the world that reveals itself when looking...by the ASM Agar Art Winners! It is absolutely fascinating to see the living art designed by creative minds...steady hand. By connecting art and science, a fascinating interface is created that produces loads of inspiration...

  6. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...your preference, we are here to make it fast and easy. Fast Approval: The UBMTA used by Addgene is ...approvals, so your plasmid requests can get to your lab faster. For Everybody: Friendly Support: Have a question...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...example, the far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal ...surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a tdTomato fluorescent...in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing... Alter chromatin architecture. Yu et al., 2020 FAST BphS from R. sphaeroides Split Cas9 Genome editing...

  8. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...is activated eight times faster than GRABNE and deactivated three times faster. This enhanced response ...sensitive to and selective for norepinephrine and faster to respond than GRABNE. While GRABNE is blocked...

  9. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...to the grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was approved...coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority of deposited materials...

  10. The MTA at Addgene

    Type
    Blog Post
    ...Addgene does a lot to ensure that we can facilitate a fast and agile MTA process. This is important to keep... last twenty years! This has helped us develop a fast, agile approach, which we’ve updated and refined...organization upon purchase. We recognize exceptionally fast tech transfer offices with the Golden Stopwatch ...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    ...primary antibodies). Direct westerns are simpler and faster than indirect ones and can be used in highly quantitative...for very large proteins. A semi-dry transfer is faster — 3–30 minutes — and uses less buffer, but it is...various manufacturers. These proprietary systems are fast (often <10 minutes) and simple, utilizing individually...fluorescent proteins. Chemiluminescence enzymes are cheap, fast, shelf-stable before activation, and light-insensitive...

  12. The time and cost required to make a plasmid

    Type
    Blog Post
    ...ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (but if ... you need a lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid...working at speed or money if budget is a concern. The faster methods, like Gibson assembly, are typically more...

  14. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Kinetics How fast is the process you would like to monitor? Neurons, for example, have unusually fast calcium...three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but slower)....

  15. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...have very different disease phenotypes, are two fascinating examples of epigenetics in the form of imprinting...lead to excessive eating and obesity. What is fascinating is that both syndromes can present without any...treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving patients ...

  16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...determined by their homology. You might also consider FastCloning, first described by Chaokun Li and colleagues...the prices of the additional enzymes required. FastCloning may be the least expensive of all the options...Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

  17. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...these viruses. The cells are also easy to cultivate, fast-growing, and widely available. The viruses Lentiviruses...like HEK-293T cells, they are easy to cultivate, fast-growing, and widely available. Unlike mammalian ...adenoviruses and the origin of HEK 293 cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096...

  18. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK.../2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...stimulate at a fast frequency without missing an action potential, then a channel with fast kinetics may...V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience, 13(3...

  20. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...accolades that would launch our careers in science. Fast forward nearly 20 years and I am known by some colleagues...in Jefferson’s case, even a lot about everything. Fast forward a few hundred years and there are currently... will not only get your paper out the door much faster, but getting helpful feedback from other people...
Showing: 61 - 80 of 270 results