Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 62 results for: Free Use GFP

Showing: 61 - 62 of 62 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... might serve as a starting point for developing useful tools. A lucky start As Andrew and Maria explained...Cohen, A. E. (2011). Mapping Nanomagnetic Fields Using a Radical Pair Reaction. Nano Letters, 11(12), 5367...protein they tested — E. coli expressing good ol’ EGFP — showed a slight but detectable change in fluorescence...better, multiple other labs have reproduced their EGFP findings since the initial report in 2023.)   ...   Figure 1: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 mT...proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable one-component... the proteins that had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random...

  2. CRISPR Guide

    Type
    Guide
    ...these PAM-flexible Cas9s are useful for precision edits, they can be used for virtually any CRISPR application...concentration too low to be used in experiments and must be amplified before use (Figure 8C), following the...libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR libraries from...co-opted for use in eukaryotic cells . The Cascade complex is recruited to target DNA using a gRNA and ...bacterial genomic region used in pathogen defense CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator...multiple genes at once; using dual nickases to generate a knockout or gene edit; or using Cas9 to generate large...will look for relevant hits using NGS technology. For more detail on using CRISPR libraries for screens...
Showing: 61 - 62 of 62 results