...endogeneous DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. ...

...editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous genes close to...

...diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822...

...These precise edits tend to be more efficient than HDR (reviewed in Zhu et al., 2019). Both cytosine base...

.... These tools assess repair events by type (NHEJ/HDR) and track indels at a single locus, usually the ...

...eukaryotes in that Cas9-mediated cleavage induces HDR to directly modify a given gene. CRISPR-tranposons...

...plus luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted...)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) This plasmid contains two expression...

...Elizabeth Hubbard: Neurons involved in opioid withdrawal Elizabeth Hubbard is a fourth year Ph.D. candidate...the development of behavioral signs of opioid withdrawal before they occur.  First, she used a genetic...genetic method to tag neurons activated by opioid withdrawal with tdTomato and assessed cFos expression. She...fluorescence during morphine administration and withdrawal to explore how these cells may encode the negative...negative affect associated with drug withdrawal.  “Together, this project will allow us to better understand... how neuron activity changes following opioid withdrawal, providing insight into new methods for treating...

...Office, between 1997 and 2000, ten drugs were withdrawn from the US market because of life-threatening...therapies. Let’s go back to why ten drugs were withdrawn from the market between 1997 and 2000. There are...

...indicating a quick cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate...

...acyl-lysine derivatives Bacterial Wenshe Liu 127445 pEDF-PhdRS PylRS N346A-C348A M. mazei Bacterial Wenshe Liu ...