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We narrowed to 278 results for: HTT

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  1. Hot Plasmids February 2024

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    ...Methods, 1–9. https://doi.org/10.1038/s41592-023-02085-6. PMID: 38036853. Preprint: https://doi.org/10.21203...Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018-w. PMID: 38081970. Preprint: https://doi.org/10.21203...Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org/10.1038/s41587-023-01756-1. PMID: 37095348...derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic...brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038/s41565-023-01419-x. PMID: 37430038...in primates. Nature Communications, 14(1), 3345. https://doi.org/10.1038/s41467-023-38582-7. PMID: 37291094...
  2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...transposon-encoded CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo...RNA-guided DNA integration. Nature 571:219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova...transposons. Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond... Bacteria Within Microbial Communities. bioRxiv https://www.biorxiv.org/content/10.1101/2020.07.17.209189v2...CRISPR-associated transposases. Science 365:48–53 . https://doi.org/10.1126/science.aax9181 Strecker J, Ladha...CRISPR-associated transposases.” Science 368:eabb2920 . https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda ...multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang Y, Sun...
  3. Negative Can Be Positive: Open AAV Data with Addgene

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    ...comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038/s41434-018-0028-5 de Backer, M...Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Gruntman, A. M., ...trials. Human Gene Therapy Methods, 26(2), 71–76. https://doi.org/10.1089/hgtb.2015.040 Initiative, F. B...Preparations. Human Gene Therapy Methods, 30(6), 214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G.,...and Clinical Development, 18(September), 98–118. https://doi.org/10.1016/j.omtm.2020.05.018 Tedersoo, L...scientific disciplines. Scientific Data, 8(1), 1–11. https://doi.org/10.1038/s41597-021-00981-0 Wright, J. ...formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016/j.ymthe.2005.02.021 Zuk, P., Sanchez...
  4. A Needle in a Base-Stack: Cas9 Structural Biology

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    .... Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739-4 References ...surveillance by CRISPR–Cas9. Nature, 603(7900), 343–347. https://doi.org/10.1038/s41586-022-04470-1 Cofsky, J. ...Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038/s41594-022-00756-0 Jiang, F., ...Mechanisms. Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822 ...Guide RNA and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Osuka, S., ... CRISPR–Cas9. The EMBO Journal, 37(10), e96941. https://doi.org/10.15252/embj.201796941 Pattanayak, V....specificity. Nature Biotechnology, 31(9), 839–843. https://doi.org/10.1038/nbt.2673 Sternberg, S. H., LaFrance...
  5. CRISPR 101: Cytosine Transversion Editors

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    ...outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:3838/BE_SMART/. Even more new DNA...repair proteins. Nature Communications, 12(1), 1384. https://doi.org/10.1038/s41467-021-21559-9 Huang, M. E...translocation. Nature Cell Biology, 26(2), 294–304. https://doi.org/10.1038/s41556-023-01342-2 Koblan, L. ...learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/s41587-021-00938-z Koblan, L. ...reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt.4172 Kurt, I. C., Zhou, R....human cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587-020-0609-x Yang, C., Dong..., Baden-Wurttemberg, Germany), 9(26), e2202957. https://doi.org/10.1002/advs.202202957 Yuan, T., Yan, ...
  6. Plasmids 101: Plasmid Incompatibility

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    ...by low-copy number plasmids. Plasmid 80:54–62 . https://doi.org/10.1016/j.plasmid.2015.03.007 Pubmed PMID...clusters. Molecular Microbiology 56:1430–1440 . https://doi.org/10.1111/j.1365-2958.2005.04643.x Pubmed...resistance plasmids in vivo. PLOS ONE 12:e0172913 . https://doi.org/10.1371/journal.pone.0172913 Pubmed PMID...Using Plasmid Incompatibility. PLoS ONE 7:e29875 . https://doi.org/10.1371/journal.pone.0029875 Pubmed PMID...incompatibility. Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111/j.1472-765x.2008.02426.x Pubmed...Current Opinion in Structural Biology 22:72–79 . https://doi.org/10.1016/j.sbi.2011.11.001 Pubmed PMID:...and host taxonomy. Frontiers in Microbiology 6: . https://doi.org/10.3389/fmicb.2015.00242 Pubmed PMID: ...
  7. CRISPR/Cas9 FAQs Answered!

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    ...Nucleases. G3 Genes Genomes Genetics, 3(4), 657–664. https://doi.org/10.1534/g3.112.005439  Cong, L., Ran,...CRISPR/CAS systems. Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143  Elliott, B.,... Molecular and Cellular Biology, 18(1), 93–101. https://doi.org/10.1128/mcb.18.1.93  Ran, F. A., Hsu...genome editing specificity. Cell, 154(6), 1380–1389. https://doi.org/10.1016/j.cell.2013.08.021  Ran, F. ...CRISPR-Cas9 system. Nature Protocols, 8(11), 2281–2308. https://doi.org/10.1038/nprot.2013.143 Sander, J. D.... genomes. Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt.2842  Soldner, F., Laganière...Parkinson Point mutations. Cell, 146(2), 318–331. https://doi.org/10.1016/j.cell.2011.06.019  Wang, H.,...
  8. Exploring Applications of the Bioluminescent HiBiT Tag

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    ...substrate. ACS Chemical Biology, 7(11), 1848–1857. https://doi.org/10.1021/cb3002478 Kamada, Y., Tateishi...Degradation of Misfolded CFTR. Cells, 12(23), 2741. https://doi.org/10.3390/cells12232741  Kamio, T., Kono...Late-Onset Gastric Cancer. Cancer Medicine, 14(8). https://doi.org/10.1002/cam4.70793 Laissue, P. P., Alghamdi...fluorescence imaging. Nature Methods, 14(7), 657–661. https://doi.org/10.1038/nmeth.4344  Peach, C. J., Kilpatrick...British Journal of Pharmacology, 178(12), 2393–2411. https://doi.org/10.1111/bph.15426 Promega Corporation ...Proteins Using HiBiT CRISPR Cell Lines. Promega Notes. https://www.promega.com/resources/pubhub/2025/illuminating-transmembrane-proteins-using-hibit-crispr-cell-lines...human tissue and in vitro. Oncology Reports, 50(5). https://doi.org/10.3892/or.2023.8631 Tsien R. Y. (1998...
  9. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ...Vulnerable Patients. Frontiers in Immunology, 13. https://www.frontiersin.org/articles/10.3389/fimmu.2022.885672Piché...Safety Assessment (pp. 519–542). Academic Press. https://doi.org/10.1016/B978-0-12-417144-2.00027-5van ... Challenges. The oncologist, 21(10), 1260–1268. https://doi.org/10.1634/theoncologist.2016-0061WHO. International...Enhancements. Frontiers in drug discovery, 2, 952326. https://doi.org/10.3389/fddsv.2022.952326Joubert, M. K...Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https://doi.org/10.1371/journal.pone.0159328Groell, F....Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang...treatment of diseases. J Biomed Sci 27, 1 (2020). https://doi.org/10.1186/s12929-019-0592-z  Resources Antibodies...
  10. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ... Function Phenotypes. Plant Biology 7:251–257 . https://doi.org/10.1055/s-2005-837597 Li J-F, Chung HS... Silencing in Plants. Plant Cell 25:1507–1522 . https://doi.org/10.1105/tpc.113.112235 Morgens DW, Deans...for essential genes. Nat Biotechnol 34:634–636 . https://doi.org/10.1038/nbt.3567 Nakaoka Y, Miki T, Fujioka...Microtubule Generation. Plant Cell 24:1478–1493 . https://doi.org/10.1105/tpc.112.098509 Smits AH, Ziebell...in CRISPR knock outs. Nat Methods 16:1087–1093 . https://doi.org/10.1038/s41592-019-0614-5 Sztal TE, Stainier...genetic robustness. Development 147:dev186452 . https://doi.org/10.1242/dev.186452 Wilson RC, Doudna JA...RNA Interference. Annu Rev Biophys 42:217–239 . https://doi.org/10.1146/annurev-biophys-083012-130404 ...
  11. Summer SciComm: Preprints

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    ...Resources References ASAPbio, Preprint Resource Center: https://asapbio.org/preprint-info ASAPbio, Preprints and... and Rapid Communication of COVID-19 research: https://asapbio.org/preprints-and-covid-19 Ettinger CL,...preprinting for early-career researchers. Biol Open. https://doi.org/10.1242/bio.059310 Fraser N, Mayr P, ...preprints: a survey of bioRxiv authors. bioRxiv. https://doi.org/10.1101/2021.09.07.459259 Sarabipour S...early career researcher perspective. PLoS Biol. https://doi.org/10.1371/journal.pbio.3000151 Sever R, ...bioRxiv: the preprint server for biology. bioRxiv. https://doi.org/10.1101/833400  Stern BM, O’Shea EK (2019...scientific publishing in the life sciences. PLoS Biol. https://doi.org/10.1371/journal.pbio.3000116 More resources...
  12. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...by an anti-CRISPR protein. Nature 546:436–439 . https://doi.org/10.1038/nature22377 Harrington LB, Doxzen...Inhibitor of CRISPR-Cas9. Cell 170:1224-1233.e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha...Therapy - Methods & Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, ...CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020-0771-6 Mayo-Muñoz D...islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695 Pawluk A, Amrani N, ...Off-Switches for CRISPR-Cas9. Cell 167:1829-1838.e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang... an anti-CRISPR DNA mimic. Sci Adv 3:e1701620 . https://doi.org/10.1126/sciadv.1701620 Additional Resources...
  13. Viral Vectors 101: Chemogenetics

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    ...Designer Drugs. ACS Chemical Neuroscience 6:476–484 . https://doi.org/10.1021/cn500325v Goutaudier, R., Coizet...Specific Ligands. Eneuro, 6(5), ENEURO.0171-19.2019. https://doi.org/10.1523/ENEURO.0171-19.2019Jendryka M,...DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee... clinical applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282...transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth BL (2016) DREADDs for...for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Saloman, J. L., ...The Journal of Neuroscience, 36(42), 10769–10781. https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston ...
  14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...CRISPR-Cas9 Genome Editing. Circ Res 115:488–492 . https://doi.org/10.1161/circresaha.115.304351 Holkers ...engineered nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin... perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/s0006297916070063 Maddalo D, Manchado...with the CRISPR/Cas9 system. Nature 516:423–427 . https://doi.org/10.1038/nature13902 Maggio I, Holkers ...in a diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038/srep05105 Stephens CJ, Lauron ...Cas9. Journal of Controlled Release 298:128–141 . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, ...Immune Responses. Human Gene Therapy 26:432–442 . https://doi.org/10.1089/hum.2015.087 Additional resources...
  15. Magnetic Control of Proteins: More than a Dream

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    ...Channels. Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016/j.bpj.2019.01.003.  Frank Hayward...the brightness of fluorescent proteins. Zenodo. https://doi.org/10.5281/zenodo.11406498. andrewgyork.github.io...magnetoreception. Protein Science, 30(8), 1521–1534. https://doi.org/10.1002/pro.4124.  Kattnig, D. R., Evans...magnetoreception. Nature Chemistry, 8(4), 384–391. https://doi.org/10.1038/nchem.2447.  Lee, H., Yang, N....Pair Reaction. Nano Letters, 11(12), 5367–5372. https://doi.org/10.1021/nl202950h.  Matsuoka, R., Kimura...American Chemical Society, 145(25), 13615–13622. https://doi.org/10.1021/jacs.3c01076.  Wheeler, M. A.,...nervous system. Nature Neuroscience, 19(5), 756–761. https://doi.org/10.1038/nn.4265.  Additional resources...
  16. Degrading DNA with Cascade-Cas3

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    ...unwinding steps. Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016/j.molcel.2018.03.031 Xiao, Y.,...degradation by Cascade and Cas3. Science, 361(6397). https://doi.org/10.1126/science.aat0839 Yoshimi, K., &...CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, ...CRISPR Cascade. Molecular Cell, 83(5), 746-758.e5. https://doi.org/10.1016/j.molcel.2023.01.024 Morisaka,...editing in human cells. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-13226-x Cameron, P....cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K..... Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/10.1038/nrmicro3569 Additional resources...
  17. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...Cassiopea xamachana. Marine Biology 94:423–430 . https://doi.org/10.1007/bf00428249 Chan MCY, Karasawa ...Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc.m606921200 Dove SG, Takabayashi...Acroporid Corals. The Biological Bulletin 189:288–297 . https://doi.org/10.2307/1542146 Lazarus, J. E., Warr, ...Applied and Environmental Microbiology, 85(16). https://doi.org/10.1128/aem.00990-19 Liljeruhm, J., Funk...biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100-0 Murakoshi, H...expression in living cells. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-48604-4 Salih A, Larkum...corals are photoprotective. Nature 408:850–853 . https://doi.org/10.1038/35048564...
  18. Hot Plasmids: Fall 2024

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    ...Blot for Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski...tag-linked antigen. J Gen Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038... isolates. J Gen Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904...of protein degraders. Nat Commun., 15(1), 8885. https://doi.org/10.1038/s41467-024-52871-9. PMID 39406745...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption...Mycobacterium abscessus. J Bacteriol., 206(3), e0033523. https://doi.org/10.1128/jb.00335-23.  Bioluminescent...Biological Systems. ACS Sensors, 9(7), 3489–3495. https://doi.org/10.1021/acssensors.3c02664. ...
  19. Hot Plasmids Spring 2024

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    ...CRISPR screens. bioRxiv 2024.03.17.585235; doi: https://doi.org/10.1101/2024.03.17.585235.   Improved...small RNA-binding protein. Nature. 2024 Apr 3. doi: https://doi.org/10.1038/s41586-024-07259-6. PMID: 38570691...specific monoclonal antibody. Virology. 558, 28–37. https://doi.org/10.1016/j.virol.2021.01.003. PMID: 33714753...JaneliaFluor dye emitting at 525 nm. Image reused from https://www.janelia.org/open-science/voltron-and-positron... al. (2023) Neuron, 111(10), 1547–1563.e9. doi: https://doi.org/10.1016/j.neuron.2023.03.009. PMID: 37015225...Adeno-Associated Virus. bioRxiv 2023.12.12.571321; doi: https://doi.org/10.1101/2023.12.12.571321.  Revolutionizing...editing and gene therapy. Nat Commun., 14(1), 6578. https://doi.org/10.1038/s41467-023-42386-0. PMID: 37852949...
  20. CRISPR 101: Any Base Transversion Editors

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    ... embryos. Nature Biotechnology, 42(4), 638–650. https://doi.org/10.1038/s41587-023-01821-9 He, Y., Zhou...base editing. Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016/j.molcel.2024.01.021 Tong, H.,...glycosylase. National Science Review, 10(8), nwad143. https://doi.org/10.1093/nsr/nwad143 Tong, H., Wang, H....glycosylase. Nature Communications, 15(1), 4897. https://doi.org/10.1038/s41467-024-49343-5 Tong, H., Wang...glycosylase. Nature Biotechnology, 41(8), 1080–1084. https://doi.org/10.1038/s41587-022-01595-6 Ye, L., Zhao...cells. Nature Biotechnology, 42(10), 1538–1547. https://doi.org/10.1038/s41587-023-02050-w Yi, Z., Zhang...glycosylase. Nature Communications, 15(1), 6397. https://doi.org/10.1038/s41467-024-50012-w Additional ...
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