CRISPR Plasmids - RNA Editing
Type
Collection
...RNA editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...
We narrowed to 327 results for: INO
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Brain Initiative Collection
TypeCollection...for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable...for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post... the prediction of T cell epitopes based on the amino acid sequence of the antibody. NetMHCPan is one...adverse reactions to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug...Immunogenicity Assessment for Sequences Containing Unnatural Amino Acids: A Method Using Existing in silico Algorithm... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...orthologs from Eubacterium siraeum (EsCas13d) and Ruminococcus sp. (RspCas13d), while Konermann et al. characterized...Anaerobic digester metagenome” (AdmCas13d) and Ruminococcus flavefaciens (nicknamed CasRx), as well as EsCas13d...with WYL domains (named for the three conserved amino acids in the domain). Yan et al. from Arbor Biotechnologies... -
Plasmids 101: Inducible Promoters
TypeBlog Post...used for bacterial protein purification. When arabinose is absent, regulatory protein AraC binds O and...pBad, blocking transcription. The addition of arabinose causes AraC to bind I1 and I2 sites, allowing ...allowing transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can... -
To Each HIS Own
TypeBlog Post...complex mix using affinity chromatography against an amino acid sequence, or tag, on the protein. While a variety...proteins. Despite being a relatively low frequency amino acid, proteins containing two or more adjacent histidines... The polyhistidine tag is a string of histidine amino acids on the N- or C- terminus of a protein. Neither... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...systems such as Cascade-Cas3, Cas9, Cas12, and Cas13 (Marino et al., 2020). Some Acr proteins are specific to...tissues The small size of Acr proteins (~50-200 amino acids) means they can be delivered in vivo using...401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, Pinilla-Redondo R, Csörgő B, Bondy-Denomy J... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...that change protein sequence affect arginine (Arg) amino acids more frequently than any other. Indeed, nearly... that cause human disease change Arg to another amino acid. Clearly, the development of a pharmacological...millions of individuals worldwide.Arg is unique among amino acids in that it possesses a guanidine moiety at... -
Of Myc and Men
TypeBlog Post...highly specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However... the epitope recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P... et al., who published a paper showing that the amino acids neighboring the Myc epitope influence 9E10... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...reach zooxanthellae, the coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000...luminescence, which require UV lamps, fluorometers, or luminometers, chromoprotein detection can be done by the ... the chromoprotein Ultramarine with hydrophilic amino acids to reduce non-specific interactions with cytosolic... -
Experimenting with New Careers while in Grad School
TypeBlog Post... Museum of Indianapolis. I drove passed these dinosaurs every day. They made me happy. Grad school can...made me happy. I started volunteering in the “Dinosphere” almost every week. I gained experience explaining...visitors of all ages, learned a lot about the dinosaurs, and met wonderful people. When I started volunteering... -
Plasmids 101: Shuttle Vectors
TypeBlog Post..., showing how DNA sequences are translated into amino acids. And I was astonished that the molecular language...But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis, and...protects against any of them. But different aminoglycosides work on different host species: kanamycin targets... -
mTOR Pathway
TypeCollection...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy levels, and growth...PIK3R1 PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and ... -
Microbiology Resources
TypeCollection...Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. ...assembly of up to three transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...non-endogenous sequences, such as a 77bp long highly recombinogenic homology sequence derived from the filamentous...used a hybrid split vector with a 872 bp long recombinogenic homology sequence to deliver LacZ to muscle...Garcia-Hoyos M, Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett ... -
TALEN Guide
TypeCollection...composed of 33-35 amino acid repeats. These repeats only differ from each other by two amino acids, their ... -
CRISPR 101: Off-Target Effects
TypeBlog Post...flexibility in gRNA placement (e.g., mutating a specific amino acid or introducing a specified edit). However, ...mutated to be more specific for exact gRNA matches (Rabinowitz and Offen). Of note, most of these optimized ...CRISPR’ing! References and Resources References Rabinowitz R, Offen D. Single-Base Resolution: Increasing... -
Zebrafish Plasmid Collection
TypeCollection...transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9...Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. .... PMID: 26390090 Other citations include: Scalabrino, et al. 2015. Intravitreal delivery of a novel AAV... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...luminescence. This can then be detected by a luminometer. Non-specific DNA stains can be added in the ...Mycoplasma Removal Agents (MRA) are derivatives of the quinolone family of antibiotics and are broad-spectrum antibiotic...culture media. Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline...