... colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields...lead to degradation of your prep. Many plasmid prep kits recommend performing an extra wash step to remove...

... you prepaid shipping materials (i.e., a deposit kit) with instructions on how to send liquid DNA or bacterial...pages for your review. We’ll also mail you a deposit kit with instructions on how to prepare and ship the ...

...and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and every contributor... for Protein Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who wrote a ...

... Kits Zinc Finger Consortium OPEN Reagents Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013...section: "The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For...1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs...Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and ...Detailed Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering... Joung Nature Protocols paper . How to Cite this Kit These plasmids were created by your colleagues. Please....2009.98. Epub 2009 Sep 17. PubMed PMID 19798082 . Kit Contents The following items will be shipped as stabs...

...mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found across all TCGA...Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in ...

...used in this study.  The zCas9i cloning kit is a plant cloning kit that contains a series of vectors and...

...Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent Protein Kits Browse the Fluorescent Protein... recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging...offers for tagging a single genetic locus, the toolkit also offers repair templates for tagging proteins... elegans, several groups have designed modular toolkits to assist with tagging genomic loci in other model...obtained as a universal donor plasmid. The CRISPaint toolkit includes several donor plasmids for protein tagging... S2 cell line stably expressing Cas9. This PCR toolkit offers C- and N-terminal tagging vectors (eg GFP... Matthew L. and Eric M. Jorgensen. “SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification...

...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting...

...the collection. Return to top Plasmid Kits for Microbiology Kits are sets of plasmids intended for use...Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating... in these model organisms. See below for plasmid kits and other resources relevant to these organisms,...listed here. Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence...subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration of DNA into prokaryotic chromosomes...chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (Rssc...Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription...

...the collection. Return to top Plasmid Kits and Collections Kits are sets of plasmids intended for use ...Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate...regarding this kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use ...Plasmids and Resources Plasmid Tools Worm Constructs Kits and Collections Other Resources The nematode Caenorhabditis...and Cre expression plasmids. Large collections or kits are described below and not included in this table...links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein...

...Gate TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The kit can be used...Bogdanove TALEN kit came online in the summer of 2011 and has already become the best-selling kit in Addgene...Hospital also recently released a TALEN kit through Addgene. This kit, first described in Nature Biotechnology...to TALEN Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally...” Using these plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 ...considerable experience in the DNA targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at...Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue...

...Biology Tet Inducible Expression Worm Expression Kits Kits are groups of plasmids that have been packaged...intended for use as a set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent... . We've gathered our curated plasmid resources, kits, and depositor collections on one page to help you...ORF. MAPK Nuclear Receptors Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific...

... Protocols RNA Extraction Without a Kit RNA Extraction Without a Kit You may also like...extraction without a kit. Protocols... like... Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction This...how to extract RNA from tissues or cells without a kit. The general steps of this protocol include homogenization... read this blog post on RNA extraction without a kit . Last Update: April 10, 2020 Equipment Refrigerated...

...mycoplasma contamination using mycoplasma detection kits. Cell line is maintained for ~20 passages before...prepared using endotoxin-free plasmid purification kits.     Figure 4: Schematic of the barcode ...

...Lentiviral Expression Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids...

...PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae...Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit Gray Lab Maize and Rice Transcription...Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular...

...working, consider using a subcellular fractionation kit or a lysing protocol developed for isolating proteins...based lysing protocol Subcellular fractional kit or lysing protocol Not enough target Run protein...Chemilumenescence: use a more sensitive exposure kit Too much signal Antibody dilutions too high...Chemilumenescence: use a less sensitive exposure kit No signal variation in highly expressed proteins...

... purification kit, such as the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow...important during the DNA purification step, as many kits cannot handle more than a certain total volume of...

...Plasmids from the Ellis Lab Find Synthetic Biology Kits Browse Our Synthetic Biology Pages ...

...over 100 empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based biosensors - Pertz Fluorescent ...Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When is a Monomer not a Monomer? Addgene's...Fusion Construct Set - Hamdoun Browse All Plasmid Kits Community Resources FPbase from Talley Lambert at...