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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 208 results for: Kars

Showing: 61 - 80 of 208 results

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

  4. Viral Production at Addgene

    Type
    Blog Post
    ...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...Mycoplasma Our 293T cell line was obtained directly from Takara, and is routinely tested for mycoplasma contamination...

  6. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...interactions. What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent...May; 34(5):341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer...

  7. Five Popular Model Organisms

    Type
    Blog Post
    ...Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism, is a commonly used model organism in...double every 2 hours. Yeast are also the first eukaryotic genome to be entirely sequenced and is very amenable...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...https://doi.org/10.5607/en20006 Rolland, A.-S., Kareva, T., Yarygina, O., Kholodilov, N., & Burke, R. ...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering...revolution shows no signs of slowing down. This prokaryotic immune system is especially amenable to genome...offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive...elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as part of an adaptive immune system...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...system seems like a winning strategy to ensure prokaryotic survival, but it is not invincible. Bondy-Denomy...provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 ...

  10. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana... M., Gotthard, G., Tünnermann, J., Tänzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...OPTN His T7 ALS Jon Ashwell 23376 pDONR223-PRKAR1B PRKAR1B Dementia and/or parkinsonism William Hahn 23452...Parkinson's, FTD Karen Ashe 46905 pRK5-EGFP-Tau AP MAPT GFP CMV Parkinson's, FTD Karen Ashe 46906 pRK5-...Parkinson's, FTD Karen Ashe 46907 pRK5-EGFP-Tau E14 MAPT GFP CMV Parkinson's, FTD Karen Ashe 46908 pRK5...Parkinson's, FTD Karen Ashe 46909 pRK5-EGFP-Tau E14 P301L MAPT GFP CMV Parkinson's, FTD Karen Ashe 46924 YFP-Parkin...-Spatz disease David Root 76838 PRKAR1B gRNA (BRDN0001146606) PRKAR1B hU6 Dementia and/or parkinsonism...parkinsonism David Root 76839 PRKAR1B gRNA (BRDN0001144765) PRKAR1B hU6 Dementia and/or parkinsonism David Root ...Root 76840 PRKAR1B gRNA (BRDN0001147089) PRKAR1B hU6 Dementia and/or parkinsonism David Root 76898 PRKCG ...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest...

  14. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...for PCR and surveyor. Q15: I used DNA polymerase Takara Ex Taq™ DNA Polymerase for my genomic PCR, but ...successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this case for EMX1...

  15. CRISPR Guide

    Type
    Collection
    ...bacterial species, but newer versions engineered for eukaryotic cells are available and further improvements ...Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is recruited to target...25122746 Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. ... Science , 339 (6121), 823–826. PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,... Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby..., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...

  16. Bacterial Expression Systems

    Type
    Collection
    ...luminescence (lux operon) Gram-negative bacteria Attila Karsi 14080 pAKlux2 Promoter activity Luminescence (lux...lux operon) Gram-negative bacteria Attila Karsi 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a...GFPmut3a) Gram-negative bacteria Attila Karsi 18855 FLIParaF.Ec-250n Arabinose FRET fluorescence (CFP and Venus...Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence ...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ... and reversible control of gene expression in eukaryotes. To avoid secondary effects from repurposing ... Tet-On vector with CMV promoter Tet-On 3G rtTA Oskar Laur 96963 pCAG-TetON-3G Mammalian expression of...2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-...(Link opens in a new window) External Resources Takara: Tet-inducible Systems Overview (Link opens in ...

  18. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... 33,170 Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919...92,919 Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ... CKR-1, CMKAR1, CXCR1, IL8R1, IL8RBA IL8RB interleukin 8 receptor, beta CD182, CDw128b, CMKAR2, CXCR2,... (C-X-C motif) receptor 3 CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR CXCR4 chemokine (C-X-C... protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2...Smith B, Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E, Wiser J, Butte AJ. ...

  20. Retrograde AAV viral preps

    Type
    Collection
    ... viral service. Retrograde construct from Alla Karpova at Janelia Research Institute....preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector... these viral preparations was performed by the Karpova lab. Results for our quality control experiments...
Showing: 61 - 80 of 208 results