...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...Mycoplasma Our 293T cell line was obtained directly from Takara, and is routinely tested for mycoplasma contamination...

...However, approximately 40% of human (and most eukaryotic) proteins localize to chemically distinct subcellular...fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway."Biochemical and biophysical...

...interactions. What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent...May; 34(5):341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer...

...Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism, is a commonly used model organism in...double every 2 hours. Yeast are also the first eukaryotic genome to be entirely sequenced and is very amenable...

...Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26975 26975-AAV1 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26966P 26966-AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26969P 26969-AAV5 pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26973P 26973-AAV5 pAAV-hSyn-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...

...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...https://doi.org/10.5607/en20006 Rolland, A.-S., Kareva, T., Yarygina, O., Kholodilov, N., & Burke, R. ...

...Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana... M., Gotthard, G., Tünnermann, J., Tänzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein...

...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest...

...Laboratory. Vancouver, BC Canada 49.2827°N 123.1207°W – Karissa Milbury I attended the march in Vancouver, BC,...science in the public sphere as often as they can. Karissa Milbury is currently a PhD candidate at the BC ...

...An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering...revolution shows no signs of slowing down. This prokaryotic immune system is especially amenable to genome...offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive...elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as part of an adaptive immune system...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...system seems like a winning strategy to ensure prokaryotic survival, but it is not invincible. Bondy-Denomy...provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 ...

...OPTN His T7 ALS Jon Ashwell 23376 pDONR223-PRKAR1B PRKAR1B Dementia and/or parkinsonism William Hahn 23452...Parkinson's, FTD Karen Ashe 46905 pRK5-EGFP-Tau AP MAPT GFP CMV Parkinson's, FTD Karen Ashe 46906 pRK5-...Parkinson's, FTD Karen Ashe 46907 pRK5-EGFP-Tau E14 MAPT GFP CMV Parkinson's, FTD Karen Ashe 46908 pRK5...Parkinson's, FTD Karen Ashe 46909 pRK5-EGFP-Tau E14 P301L MAPT GFP CMV Parkinson's, FTD Karen Ashe 46924 YFP-Parkin...-Spatz disease David Root 76838 PRKAR1B gRNA (BRDN0001146606) PRKAR1B hU6 Dementia and/or parkinsonism...parkinsonism David Root 76839 PRKAR1B gRNA (BRDN0001144765) PRKAR1B hU6 Dementia and/or parkinsonism David Root ...Root 76840 PRKAR1B gRNA (BRDN0001147089) PRKAR1B hU6 Dementia and/or parkinsonism David Root 76898 PRKCG ...

...bacterial species, but newer versions engineered for eukaryotic cells are available and further improvements ...Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is recruited to target...25122746 Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. ... Science , 339 (6121), 823–826. PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,... Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby..., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...

... 33,170 Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919...92,919 Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ...

...Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex ...

...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h...

...selection markers (for use in other cell types, e.g. eukaryotic cells). pBACKBONE-XXXXX Tip: For a catalog of...

... the researchers led by Dr. Jorge Ruas of the Karolinska Institutet, the discovery really wasn’t what ...

... CKR-1, CMKAR1, CXCR1, IL8R1, IL8RBA IL8RB interleukin 8 receptor, beta CD182, CDw128b, CMKAR2, CXCR2,... (C-X-C motif) receptor 3 CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR CXCR4 chemokine (C-X-C... protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2...Smith B, Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E, Wiser J, Butte AJ. ...

...avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities with PUF proteins...