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Showing: 61 - 80 of 177 results
  1. Western Blot

    Type
    Protocol
    ...out of the wells and into the gel. Increase the voltage to 150 V and continue running the gel until you...
  2. CRISPR Library Amplification

    Type
    Protocol
    ...Caution Electroporation involves the use of high voltages, please use caution when activating pulse and ...
  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but does not...complement oligo ( e.g., sgRNA-A in Table 4 ). The resultant oligos would be 25-mer oligos. However, if the...the reverse complement oligo. In this case, the resultant oligos would be 24-mer oligos ( Table 4 ). Design...
  4. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...study shows that the CRISPR/Cas9 system enables simultaneous disruption of two target genes in one step without...
  5. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo brain microdialysis: advances...
  6. Genetic Code Expansion

    Type
    Collection
    ...(AzK) Mammalian TAG Abhishek Chatterjee 218764 pEvoltac-EcW-TGA-h14 TrpRS-h14 (S8A, V144G, V146C) E. coli... coli Bacterial TGA Abhishek Chatterjee 218765 pEvoltac EcW-TGA-h9 TrpRS-h9 (S8A, V144S, V146A) E. coli... RF1 function removed Michael Jewett 73581 C321.deltaA (Isaacs lab) all TAG sites changes to UAG, RF1 ...
Showing: 61 - 80 of 177 results