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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 139 results for: MAX

Showing: 61 - 80 of 139 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional allele...Selection of gRNAs for an experiment needs to balance maximizing on-target activity while minimizing off-target...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...population level, but each individual cell only has a maximum of one gene knocked out. In this genetically heterogeneous...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier name CGBE1, for....2% of studied sequences) (Huang et al., 2024). BE4max, a CBE, generated similar deletions at a lower ...

  4. Plan Your Experiment

    Type
    Collection
    ... it is also important to consider factors that maximize cleavage of the desired target sequence, or on-target...

  10. Hot Plasmids - October 2022

    Type
    Blog Post
    ...potassium-selective channelrhodopsins. HcKCR1 has a maximum absorption of 540 nm and two-photon excitation ...
Showing: 61 - 80 of 139 results