...scientists need a protease reporter that has a large dynamic range and high brightness. The Shu Lab from UCSF...fluorescence range to better study the spatio-temporal dynamics of apoptosis in living animals. To do this they... by flipping a single β-strand of GFP, hence the name FlipGFP. FlipGFP increases fluorescence 100-fold...Shu Lab used FlipGFP to track the spatiotemporal dynamics of apoptosis during embryo development. GFP fluorescence... a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter achieves...scientists to observe both the spatial and temporal dynamics of apoptosis both in vitro and in living organisms...

...Methods. 2022 Mar 28. Follow APP processing and dynamics using APP and Bace1 plasmids . Capitini et al....

...sample or protein of interest to understand the dynamic or working range of a given reagent (usually an...protein concentrations are within the antibody’s dynamic range. Created with biorender.com   Alternatively...recommended dilution. If the antibody dilution’s dynamic range encompasses your protein’s expected concentrations...dilution until your protein dilutions are in the dynamic linear range (i.e., expression decreases as protein....     Figure 3: Testing the antibody’s dynamic range. A) The protein dilutions, representing expected... concentrations, are all within the antibody’s dynamic range. B) The protein dilutions, representing expected... concentrations, are outside of the antibody’s dynamic range. Specifically, the 1:2, 1:1, and No dilution...

... creating the SunTag system. The SunTag system, named after the "stellar explosion SUperNova", is a synthetic...contains a nonfunctional truncated version of the kanamycin resistance gene (missing the first two codons)...pUCXKT will therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False... allows the study of loci proximity and of the dynamic interactions between intra- and inter-chromosomal...LOC8R-PAGFP provides a novel strategy for assaying the dynamics of different mitochondrial subpopulations within... Ed Boyden’s lab. The enhanced sdChr construct, named CheRiff, exhibits high light sensitivity and fast...changes reduced system leakiness and increased the dynamic range, resulting in more user-friendly and tunable...

...nucleases to the zebrafish community. Target Gene Name TALENs Full Target Site (5' to 3'; half-sites in...

...broad dynamic range that typically spans 2–5 orders of magnitude, which includes a wide dynamic range ... may be light-sensitive. They also have a poor dynamic range, meaning that if your blot contains one protein...for multiplexing. They have a ten-fold greater dynamic range than chemiluminescence enzymes, making it...developer. Film is highly sensitive but has a limited dynamic range, which means it may take multiple exposures...background Chemiluminescence reporter Smaller dynamic range; no multiplexing Protein-based blocker...background Chemiluminescence reporter Smaller dynamic range; no multiplexing Wet transfer Increased...

...to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek mythology) switches...PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term for vanishing, and pa, ...trafficking, as well as organelle trafficking and dynamics (fission, fusion, etc). For example, by engineering... was able to visualize and quantify the fusion dynamics of mitochondria in healthy and apoptotic cells...at their fingertips the tools to probe cellular dynamics and function with extraordinary molecular detail...Mariusz, et al. "Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows...

...AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI ...

...resistance gene, you’ll find the full name of the gene, the name of its gene product, and a description...cut the given sequence. The table includes enzyme names, the locations where the enzymes will cut (in basepairs...the plasmid sequence. The table includes feature names, locations, sizes, colors used to indicate them ... nucleotide sequence. The table includes primer names, sequences, binding site locations, lengths, and...

...for dTAG molecules and any questions to Behnam Nabet (behnam_nabet@dfci.harvard.edu), Nathanael Gray (nathanael_gray...This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer ...et al.), and in vivo studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber... the dTAG technology. Follow him on twitter @behnamnabet.   References Nabet et al. The dTAG system for...

... in your database to the effect of `dev_name` and `science_name`. You'll need to decide given the technical...things tracked. Terminology and language are tricky Names for things will trip everyone up at some point, ..., decide early on what to do in a namespace collision. It may even be worth it to have extra columns in... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

...sequence or name. You can perform more in-depth analyses on each sample by clicking on the sample name or on...and provide basic information such as your sample names and guide sequences, and ICE will do the rest. There...shows a green checked circle in front of the sample name. If there was a minor error during processing, the...following parameters: Sample - The unique label name that you provided for each sample. Guide Target ...

...Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-GFP ...

...bright Recent series of enhanced monomeric NIR FPs named miRFPs (monomeric infrared FPs) (Matlashov et al...recommend using miRFPs and their enhanced versions named emiRFPs in your applications.  There is a whole ...distinct (e)miRFPs available. The numbers in (e)miRFP names correspond to the maxima of their emission spectra....  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome... An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11...

... to control the cytoskeleton and cell membrane dynamics. First, a quick refresher.  The tools below use...microtubule-severing protein, Katanin is of course named for the samurai sword called a katana. Opto-katanin...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, and more, often...proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and many functions... the many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-...

...MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 🤗 We hope everyone... tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria...bacteria, fungi, viruses, and one microbe ornament even dyed with real microbial pigment. Check out the hashtag...hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6 — Dr...

...have different names, and the software can only extract gRNAs for genes using the name that is used by...condensed text file containing a full and a short name for each gRNA, as well as the suggested gRNA sequence...one solution is to identify all the alternative names for your gene of interest (that were not found by...Green Listed), and test if any of the alternative names works better. To achieve this you could e.g. copy...

...Visualization of DA release dynamics during locomotion in freely moving mice Record DA dynamics of optogenetically... single neuron in vivo in Drosophila Detect DA dynamics in response to a threatening looming stimulus ...et al. "Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science...

...within an organism.  So how did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the...and François Wollman and became the widely adopted name for these elements. At the time, the use of episome...resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected...store, QC, curate, and distribute them all in the name of making it a little bit easier for scientists ...

...Blackburn EH, Weissman JS, Qi LS, Huang B. 2013. Dynamic imaging of genomic loci in living human cells by...