...variants. VKOR is an enzyme critical for activating clotting factors and is the target of the anticoagulant...develop an artificial leaf replica system. “This bottom-up approach will allow me to create a fully controllable...

...PubMed PMID: 15262411. 4. T. D. Southall, D. A. Elliott, A. H. Brand, The GAL4 System : A Versatile Toolkit...703–709 (2006). PubMed PMID: 16582903. 8. C. J. Potter, B. Tasic, E. V Russler, L. Liang, L. Luo, The ...

... Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441...Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26;...

...Slaviero AN, Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender...

...monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed MitoTimer plasmids... can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter ... to orange) protein. Image courtesy of Roberta Gottlieb. Laker et al., J Biol Chem 2014 Apr 25;289...

... maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are sold in packages of ...

...Resources Images used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu...robertsonlab/assets/user-content/documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide...

...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you...and may all your NHEJ mutations be out of frame! Scott Findlay is currently a Postdoctoral Fellow at the...

...expression of hM4Di in plasma membranes in both species (bottom panel in Fig. 1). Our results indicate that the...reveal the hM4Di coupled to mCherry (top) or HA tag (bottom). The images show examples of dendrites (blue shades...

...other proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted...aqueous layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate...

...was recently deposited with Addgene by Dr. Mark Prescott. This system was developed for monitoring and ... Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott, M., & Devenish, R. J. (2008). Rosella: A fluorescent...

...might want to consult the Cheeky Scientist. Their motto is “We Turn Academics into Confident and Successful...English and Spanish. She is based in Spain. The bottom line – there are lots of people out there who want...

... used to confirm protein expression by western blotting in other experiments (Figure 2).   Complementation...students about restriction mapping and western blotting. Students who participate in the course show significant...

...between the identical loxP or lox511 sites (Figure 1, bottom). Since only one loxP and lox511 site will remain...Sabatini Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham...

... spillover look like and how do I fix it? When plotting two channels against each other, you should be...single-color APC control, with APC and APC-CY7 plotted against each other. Since it is a single-color ...

... R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor supports high density... Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau V. Antibody capture process based on magnetic ...

...meeting educational goals (Kowalski et al., 2016; Scott, 2021). In fact, completion of Provost’s CURE increased...Academy of Sciences, U.S.A. 117 (25):14077-14083. Scott, K. 2021. Classroom undergraduate research experiences...

...Plasmid #141386-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein...or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading control (right...

...more about Western blotting check out our blog post The Basics of Western Immunoblotting.) For mass spectrometry...

...BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann...10.1016/j.cell.2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O,...