Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 290 results for: Ott;

Showing: 61 - 80 of 290 results

  4. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441...Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26;...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed MitoTimer plasmids... can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter ... to orange) protein. Image courtesy of Roberta Gottlieb. Laker et al., J Biol Chem 2014 Apr 25;289...

  6. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you...and may all your NHEJ mutations be out of frame! Scott Findlay is currently a Postdoctoral Fellow at the...

  7. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...Resources Images used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu...robertsonlab/assets/user-content/documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide...

  8. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are sold in packages of ...

  9. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...was recently deposited with Addgene by Dr. Mark Prescott. This system was developed for monitoring and ... Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott, M., & Devenish, R. J. (2008). Rosella: A fluorescent...

  11. RNA Extraction Without A Kit

    Type
    Blog Post
    ...other proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted...aqueous layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate...

  12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...might want to consult the Cheeky Scientist. Their motto is “We Turn Academics into Confident and Successful...English and Spanish. She is based in Spain. The bottom line – there are lots of people out there who want...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...between the identical loxP or lox511 sites (Figure 1, bottom). Since only one loxP and lox511 site will remain...Sabatini Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham...

  15. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ... spillover look like and how do I fix it? When plotting two channels against each other, you should be...single-color APC control, with APC and APC-CY7 plotted against each other. Since it is a single-color ...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor supports high density... Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau V. Antibody capture process based on magnetic ...

  17. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...meeting educational goals (Kowalski et al., 2016; Scott, 2021). In fact, completion of Provost’s CURE increased...Academy of Sciences, U.S.A. 117 (25):14077-14083. Scott, K. 2021. Classroom undergraduate research experiences...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Plasmid #141386-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein...or #211757 (middle panel). Samples were also immunoblotted against vinculin as a loading control (right...
Showing: 61 - 80 of 290 results