...these indicators difficult to work with.  The Gottschalk lab has surveyed a range of microbial rhodopsin...previously reported for other organisms, and the Gottschalk group hypothesizes that this may be due to different...

... Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441...Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26;...

...monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed MitoTimer plasmids... can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer reporter ... to orange) protein. Image courtesy of Roberta Gottlieb. Laker et al., J Biol Chem 2014 Apr 25;289...

...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you...and may all your NHEJ mutations be out of frame! Scott Findlay is currently a Postdoctoral Fellow at the...

... maybe not all of them. We still haven’t gotten to the bottom of why hot dogs are sold in packages of ...

...Resources Images used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu...robertsonlab/assets/user-content/documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide...

...was recently deposited with Addgene by Dr. Mark Prescott. This system was developed for monitoring and ... Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott, M., & Devenish, R. J. (2008). Rosella: A fluorescent...

...other proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted...aqueous layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate...

...expression of hM4Di in plasma membranes in both species (bottom panel in Fig. 1). Our results indicate that the...reveal the hM4Di coupled to mCherry (top) or HA tag (bottom). The images show examples of dendrites (blue shades...

...might want to consult the Cheeky Scientist. Their motto is “We Turn Academics into Confident and Successful...English and Spanish. She is based in Spain. The bottom line – there are lots of people out there who want...

...resulting light emissions. In FACS, cells are then slotted in a specific direction based on the emission(s...

...Slaviero AN, Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender...

... used to confirm protein expression by western blotting in other experiments (Figure 2).   Complementation...students about restriction mapping and western blotting. Students who participate in the course show significant...

...between the identical loxP or lox511 sites (Figure 1, bottom). Since only one loxP and lox511 site will remain...Sabatini Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham...

... spillover look like and how do I fix it? When plotting two channels against each other, you should be...single-color APC control, with APC and APC-CY7 plotted against each other. Since it is a single-color ...

... R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor supports high density... Schwarz H, Eriksson PO, Eriksson K, Shokri A, Chotteau V. Antibody capture process based on magnetic ...

...meeting educational goals (Kowalski et al., 2016; Scott, 2021). In fact, completion of Provost’s CURE increased...Academy of Sciences, U.S.A. 117 (25):14077-14083. Scott, K. 2021. Classroom undergraduate research experiences...

...caterpillar once, vomit, and never do it again. Cichlids (Scott Juntti) Cichlids are 2,000 fish species that evolved...Animal husbandry of diverse species is a real bottleneck. New gif for #MolluscMonday: #Crepidula atrasolea...

...eating one before your chalk talk. Bring your own bottle of water. If possible, write down your short aims...did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health...

...quantitative DNA hybridization method (DNA dot blotting) had been used to titrate AAV, but this method...8610.4. Hindson CM, Chevillet JR, Briggs HA, Gallichotte EN, Ruf IK, Hindson BJ, Vessella RL, Tewari M...