We narrowed to 82 results for: Plin

1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ... in the depletion of essential genes. In the subsampling analysis of the sgRNAs, Brunello even with only...

2. A Career in Grants: How To Become a Grant Professional

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   ...such as research, collaboration across multiple disciplines, writing, project management, and program evaluation...

3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...in research across a vast array of biological disciplines and scientists employ GFP for a wide number of...

4. Transferable Skills Guide: External Collaborations

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   ...early-career scientists provided a crash course in this discipline. Getting companies to come to these types of ...

5. Plasmids 101: Broad Host Range Plasmids

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   ...processes, and can be used by scientists across disciplines.  What confers broad host range in plasmids? ...

6. The Challenges of Cell Culture

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   ...may certainly have been a valid concern for the discipline (or lack thereof) in times gone past but these...

7. Important Considerations in Optogenetics Behavioral Experiments

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   ...- find a lab within your own neuroscience sub-discipline that has published using the technique and ask...

8. Data Freedom: The Expansion of Data Sharing in Research Publications

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   ...essential for efficient science. Improvements in our discipline for annotation and archiving of data are likely...

9. Harnessing Bacterial Toxins for Allelic Exchange

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   ...PubMed PMID: 15109831. Pyne, Michael E., et al. "Coupling the CRISPR/Cas9 system with lambda red recombineering...

10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    ..., such as the tamoxifen-responsive CreERT, the coupling of Cre-lox with FLP-FRT recombination to generate...

11. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways...

12. Viral Vectors 101: Optogenetic Tools

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    ...signaling pathways with light. This is achieved by coupling endogenous signaling molecules with light-activated...

13. Is this the right place for me? 8 tactics for choosing a lab

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    ...regarding lack of effort, intimidating use of discipline or competence procedures Threats to personal ...

14. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ... silencing of APT is required for survival. By coupling silencing of APT and any gene target of interest...

15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ... changed for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar...

16. Which Fluorescence Microscopy Technique is Best for Me?

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    ...mouse brain with a 20x/1.0NA objective with proper sampling. Therefore, large, cleared tissues need to be ...

17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    ...antibodies. Example applications of this include coupling to an antibody, whereby excitation of blue light...

18. Special Delivery: Fluorophore Targeting for FRET Studies

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    ... acceptor. Binding affinity can be enhanced by coupling two NTA molecules to each Cy dye, as well as by...

19. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...2013. 31(1): p. 82-94. PubMed PMID: 23329641. 36. Jopling, C., S. Boue, and J.C. Izpisua Belmonte, Dedifferentiation...

20. CRISPR 101: Cytosine and Adenine Base Editors

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    ...Komor created the first cytosine base editor by coupling a cytidine deaminase with the inactive dCas9 (...