We narrowed to 221 results for: Rras
-
TypeCollection...developmental transcriptome information, prenatal LMD microarray data, ISH image data, and anatomic reference ..., 944–950. PMID: 21154482 Neul et al. 2019. The array of clinical phenotypes of males with mutations in...
-
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...the spatial and temporal arrangement of chromosomes and how these arrangements may affect nuclear processes... -
A Deep Dive into BioBuilder
TypeBlog Post... from the Caltech lab of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription... -
Hot Plasmids - February 2022
TypeBlog Post...guides require two arrays for Cas9, two guides can be streamlined into a single array for Cas12a. Image... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...neurotransmission. These receptors can be activated by an array of different ligands, including hormones, neurotransmitters... showing no constitutive activity. There are an array of DREADDs that activate different G proteins, work... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...successful outcome. How do I find a coach? Start with referrals. Are there scientists you know who have had a ...offices or professional organizations can provide referrals. There are bad coaches out there, so finding a... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post... the (1) binding of calcium, (2) allosteric rearrangements of the sensor, and (3) subsequent change in... with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous calmodulin... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A toolbox of..., Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...into two categories: probes and editors. A probe array may consist of at least two sensor modules targeting...via CRISPR techniques. This tool-kit includes an array of FPs as well as additional useful tags, including... -
Hot Plasmids February 2024
TypeBlog Post...bright and stable fluorescence of the original (Ivorra-Molla et al., 2023). They also named their variant...: https://doi.org/10.21203/rs.3.rs-2941917/v1. Ivorra-Molla, E., et al. (2023). A monomeric StayGold ... -
6 Tips for Grant Writing
TypeBlog Post...post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No matter ... Many thanks to our Guest Blogger, Sean Mac Fhearraigh! Seán was a PhD student at University College... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...-020-00361-z Grimm, D., Kern, A., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration...https://doi.org/10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J.,... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support...References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...later modified by others to produce Murray’s clear solution. Murray's clear (11) was mostly used to study... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post... that may have warranted their own chapters, and certainly chapters that could warrant their own books... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post...single plasmid containing a multi-spacer CRISPR array. Workflow and technical considerations Making...simple workflow: Clone spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon... -
Lentiviral Vector Uses and Overview
TypeBlog Post...contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer vectors..., Cavallesco R, Gillet-Legrand B, Caccavelli L, Sgarra R, Maouche-Chrétien L, Bernaudin F, Girot R, Dorazio... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...Cpf1 multiplexing approach using a single crRNA array to express up to 4 crRNAs. Zetsche et al. also suggest...Gene Editing by CRISPR–Cpf1 Using a Single crRNA Array." Nat Biotechnol. 35.1 (2016): 31-34. PubMed PMID... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post...makers have moved away from long written proposal narratives. Now they require grant seekers to use online...demand for stronger budgets with clear budget narratives, logic models, established partnerships and collaborations...